Surveillance of prion protein (PrP9 gene polymorphisms in Turkish native sheep breeds

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2012

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Uzun, Begüm

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Scrapie is an infectious fatal disease of sheep and goats which affects the central nervous system. In the present study, samples of 14 native Turkish sheep breeds (n=655) were analyzed with respect to their polymorphisms of PrP gene (at codons: 136, 141, 154 and 171) and their classical and atypical scrapie risk levels were identified. Turkish sheep are found to have the highest PrP genetic variability with 13 classical scrapie alleles and 14 atypical scrapie alleles compared to all previous studies. Classical scrapie-susceptible and wild-type ARQ allele was found as the most frequent allele in Turkish sheep examined. The most classical scrapie-susceptible allele, VRQ was detected at low frequencies in 5 of the breeds (Çine Çaparı, Dağlıç, Kıvırcık, Karayaka and Gökçeada). One novel allele (TL141HQ) was observed in Sakız breed for the first time in this study. It was found that most of the classical scrapie genotypes belong to R3 risk group, whereas atypical scrapie genotypes belonging to zero (0) and one (1) risk groups were frequently seen in sheep analyzed. In other words, Turkish sheep is found to have intermediate risk of classical scrapie and low atypical scrapie risk, in general. The data from the current study may help to establish a breeding program for classical scrapie control in Turkey and will be beneficial for both the animal and public health in the country. In addition, the outcomes of the study will fill the gap which is present in the geographic distribution data of PrP gene polymorphisms in Eurasia.

Subject Keywords

Sheep breeds., Genetic polymorphisms., Prions.

URI

http://etd.lib.metu.edu.tr/upload/12614353/index.pdf
https://hdl.handle.net/11511/21594

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Graduate School of Natural and Applied Sciences, Thesis

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Citation Formats

B. Uzun, “Surveillance of prion protein (PrP9 gene polymorphisms in Turkish native sheep breeds,” M.S. - Master of Science, Middle East Technical University, 2012.