Bioactive surface design based on conducting polymers and applications to biosensors

Ekiz, Fulya
An underlying idea of joining the recognition features of biological macromolecules to the sensitivity of electrochemical devices has brought the concept of biosensors as remarkable analytical tools for monitoring desired analytes in different technological areas. Over other methods, biosensors have some advantages including high selectivity, sensitivity, simplicity and this leads to solutions for some problems met in the measurement of some analytes. In this context, conducting polymers are excellent alternatives with their biocompatibility and ease of applicability for an efficient immobilization of biomolecules in preparing biosensors. Using several materials and arranging the surface properties of the electrodes, more efficient and seminal designs can be achieved. In this thesis, it is aimed to create new direct biosensors systems for the detection of several analytes such as glucose and pesticides thought to be harmful to the environment. Recently synthesized conducting polymers (polyTBT); (poly(2-dodecyl-4,7-di(thiophen-2-yl)-2H-benzo[ d][1,2,3]triazole) and (poly(TBT 6 -NH2 ); poly(6-(4,7-di(thiophen-2-yl)-2H-benzo[d][1,2,3]triazol-2-yl)hexan-1-amine) were utilized as a matrices for biomolecule immobilization. After successful electrochemical deposition the polymers on the graphite electrode surfaces, immobilization of glucose oxidase (GOx) and choline oxidase (ChO) were carried out. Amperometric measurements were recorded by monitoring oxygen consumption in the presence of substrates at -0.7 V. The optimized biosensors showed a very good linearity with rapid response times and low detection limits (LOD) to glucose and choline. Also, kinetic parameters, operational and storage stabilities were determined. Finally, designed biosensor systems were applied for glucose and pesticide detection in different media.


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Söylemez, Saniye; Toppare, Levent Kamil; Department of Chemistry (2018)
In this thesis, two types of sensors: Conjugated polymer based amperometric biosensors and a chemiresistive sensor (polymer/SWCNT) for clinical and food applications were designed, fabricated and characterized. Three chapters in this thesis focused on ethanol, glucose and cholesterol biosensors based on functional polymer/MWCNT composition, different peptide sequence containing polymer, and also copolymer of two different functional monomers, respectively. After successful electrochemical deposition of the ...
Bioactive Surface Design Based on Functional Composite Electrospun Nanofibers for Biomolecule Immobilization and Biosensor Applications
Uzun, Sema Demirci; Kayaci, Fatma; UYAR, Tamer; TİMUR, SUNA; Toppare, Levent Kamil (2014-04-09)
The combination of nanomaterials and conducting polymers attracted remarkable attention for development of new immobilization matrices for enzymes. Hereby, an efficient surface design was investigated by modifying the graphite rod electrode surfaces with one-step electrospun nylon 6,6 nanofibers or 4% (w/w) multiwalled carbon nanotubes (MWCNTs) incorporating nylon 6,6 nanofibers (nylon 6,6/4MWCNT). High-resolution transmission electron microscopy study confirmed the successful incorporation of the MWCNTs in...
Impact damage sensing of multiscale composites through epoxy matrix containing carbon nanotubes
Arronche, Luciana; La Saponara, Valeria; Yesil, Sertan; Bayram, Göknur (2013-06-05)
Carbon nanotubes are used to provide increased electrical conductivity for polymer matrix materials, thus offering a method to monitor the structure's health. This work investigates the effect of impact damage on the electrical properties of multiscale composite samples, prepared with woven fiberglass reinforcement and epoxy resin modified with as-received multi-walled carbon nanotubes (MWCNTs). Moreover, this study addresses potential bias from manufacturing, and investigates the effectiveness of resistanc...
Biosensing Devices: Conjugated Polymer Based Scaffolds
Soylemez, Saniye; Kesika, Melis; Toppare, Levent Kamil (2019-01-01)
Conjugated polymer (CP)-based biosensors are recognized to be a next generation building architecture for highly sensitive and fast biosensing systems. This entry highlights an overview on the recent expansion of research in the field of CP-based biosensors. We start by first introducing some of the important knowledge about the CPs and biosensors. This is followed by emphasizing the importance of CPs in biosensor construction. Lastly, an overall survey of CP-based biosensor studies reported between 2010 an...
Biosensor applications of chiral metamaterials for marrowbone temperature sensing
Sabah, Cumali; Dincer, Furkan; KARAASLAN, MUHARREM; Bakir, Mehmet; ÜNAL, EMİN; Akgol, Oguzhan (2015-11-22)
In this paper, we have designed, simulated, and analyzed a microwave biosensor based on chiral metamaterials (MTMs) to detect pig biological tissues which are widely used as a trial for medical explorations and researches. There are various types of biosensor structures based on MTMs in the literature. However, only a limited number of study is conducted on biosensors based on chiral MTMs; therefore, our study is one of the pioneer works for this type of research. The proposed diamond-shaped resonator-based...
Citation Formats
F. Ekiz, “Bioactive surface design based on conducting polymers and applications to biosensors,” M.S. - Master of Science, Middle East Technical University, 2012.