Molecular characterization of salmonella isolates from animals and various foods /

Durul, Bora
Salmonella, which is a Gram-negative human and animal pathogen, poses a significant threat to public health with its existence and survival in the farm to fork chain. Of the greater importance, Salmonella enterica subsp. enterica, which covers 60 % of nearly 2500 Salmonella serotypes, causes diseases in mammals with varying host affiliations and pathogenity levels. In order that consumer safety can be assured, elimination of this harmful pathogen from the farm to fork chain is a must, thereby bringing the importance of the comprehensive investigations of the diversity of Salmonella pathogen. The objective of this research is to use genotypic and phenotypic methods to have a better understanding on the Salmonella diversity by analyzing samples from animals and various foods. Thus, for this aim, firstly, samples were collected from street food and also animal clinical cases. The final goal was to gain 50 food Salmonella isolates and 50 Salmonella animal isolates, at least, during four seasons of a year in the pilot region, Şanlıurfa. Presence of Salmonella suspected colonies was investigated through conventional methods. Then, suspected colonies were confirmed as Salmonella by using polymerase chain reaction (PCR) of Salmonella specific gene, invA. Following this, confirmed colonies were analyzed by phenotypic and genotypic methods. For the phenotypic analysis, a classical method, serotyping, was used. Moreover, for the genotypic analysis part, multi locus sequence typing (MLST), one of the most preferred genotype-based methods, providing both a high discriminatory power among subtypes and an extensive phylogenetic analysis, was utilized according to seven gene MLST (aroC, dnaN, hemD, hisD, purE, sucA and thrA) scheme designed by University College Cork. Then, phylogenetic trees of the collected isolates were constructed by using bioinformatics software. As a result of the study, 59 food-origin and 53 animal-origin isolates were recovered from 192 food samples and 355 animal fecal samples, which mean that 30.1 % of food samples and 14.9 % animal samples were Salmonella-positive. Furthermore, while MLST and serotyping had congruent discriminatory power in food isolates, MLST provided intra-serotype discrimination within several serotypes in animal isolates. On the other hand, results of both subtyping methods indicate a possible affiliation between several subtypes and their source. Moreover, in addition to 4 previously unidentified sequence types that were discovered by MLST in this study, subtype Telaviv (ST 1068) is noteworthy owing to its high prevalence in food and animal samples in this study and its large absence in the scientific literature and other surveillance studies. In conclusion, combining the power of genotype-based MLST and phenotype-based serotyping, this study provided us a clue about the unique Salmonella diversity in the pilot region. In addition to being a pioneer surveillance study in Turkey where genotypic and phenotypic approaches are utilized together, results of this study are useful in the maintenance of a new publicly available pathogen database as well as in further outbreak investigations.


Structural Insights into Alternate Aggregated Prion Protein Forms
POLANO, maurizio; Bek, Alpan; BENETTİ, federico; lazzarino, marco; LEGNAME, giuseppe (Elsevier BV, 2009-11-13)
The conversion of the cellular form of the prion protein (PrPC) to an abnormal, alternatively folded isoform (PrPSc) is the central event in prion diseases or transmissible spongiform encephalopathies. Recent studies have demonstrated de novo generation of murine prions from recombinant prion protein (recPrP) after inoculation into transgenic and wild-type mice. These so-called synthetic prions lead to novel prion diseases with unique neuropathological and biochemical features. Moreover, the use of recPrP i...
Molecular evaluation and antimicrobial susceptibility testing of Escherichia coli isolates from food products in Turkey
Kyere, Emmanuel Owusu; Bulut, Ece; AVŞAROĞLU ERKAN, MÜRŞİDE DİLEK; Soyer, Yeşim (Springer Science and Business Media LLC, 2015-06-01)
Some strains of Escherichia coli can be important food borne pathogens. Characterization and antimicrobial resistance testing of 28 E. coli isolates from random food samples obtained in Van, Turkey were performed. Primers for 6 indicator genes (fliC, stx1, stx2, eae, hlyA, and rfbE) for shiga toxin-producing E. coli and 5 indicator genes for each pathogroup (bfpA, aggR, ipaH, daaD, st, and lt) were used. E. coli isolates were also typed using pulsed field gel electrophoresis with the XbaI restriction enzyme...
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Çam, Dilek; Öktem, Hüseyin Avni; Department of Biology (2016)
Salmonella and E.coli, food pathogens, are among the very important pathogens threating the health. Rapid and easy detection of these pathogens is crucial. In this context, lateral flow assay (LFA) platform prepared by gold nanoparticles (GNPs) based on antibody for Salmonella and E.coli O157:H7 was developed in the first part of this study. In second part, single chain variable fragments (scFv) and SNAP-Tagged full IgG (fusion protein) of se155-4 antibody specific to Salmonella were genetically produced. T...
Development of rapid dipstick assay for food pathogens, Salmonella, by optimized parameters
Çam, Dilek; Öktem, Hüseyin Avni (Springer Science and Business Media LLC, 2018-10-25)
Salmonella is among the very important pathogens threating the human and animal health. Rapid and easy detection of these pathogens is crucial. In this context, antibody (Ab) based lateral flow assays (LFAs) which are simple immunochromatographic point of care test kits were developed by gold nanoparticles (GNPs) as labelling agent for Salmonella detection. For that purpose some critical parameters such as reagent concentrations on the capture zones, conjugate concentrations and ideal membrane type needed f...
Molecular evaluation and antimicrobial susceptibility testing of escherichia coli isolates collected from various food products in Turkey/
Kyere, Emmanuel Owusu; Soyer, Yeşim; Avşaroğlu, Dilek; Department of Biotechnology (2014)
Even though Escherichia coli (E. coli) is part of the intestinal microflora of healthy human beings and warm blooded animals, some strains of E. coli can be important food borne pathogens that can cause a wide spectrum of diseases, ranging from self-limiting to life threatening intestinal and extra-intestinal illnesses. Pathogenic E. coli that affect the intestines of humans have been grouped into six main pathotypes: Shiga-toxin-producing E. coli (STEC); of which enterohaemorrhagic E. coli (EHEC) is a path...
Citation Formats
B. Durul, “Molecular characterization of salmonella isolates from animals and various foods /,” M.S. - Master of Science, Middle East Technical University, 2014.