Controlled doxorubicin delivery from photoresponsive liposomes carrying vitamin A derivatives /

Download

index.pdf

Date

2014

Author

Heper, Senem

Metadata

Show full item record

Item Usage Stats

199
views

71
downloads

Drug delivery systems (DDS) have been an attractive approach to eliminate the drawbacks of conventional drug administration. Controlled and photoresponsive drug delivery systems have a special advantage; they deliver drugs more effectively. Liposomes are mostly preferred as drug carriers due to their ability to carry both hydrophilic and hydrophobic drugs, their being non-toxic and non-immunogenic. In this study, photoresponsive liposomes were prepared by incorporating vitamin A derivatives into the lipid bilayer of the liposomes for use in treatment of eye diseases that require frequent and continuous drug administration. Proliferative vitreoretinopathy was selected as a model disease and doxorubicin as the therapeutic agent. Doxorubicin loaded phosphatidyl choline (PC): cholesterol (CHOL): all-trans retinal (ATR) (7:1:3) liposomes were tested on RPE/D407 cells to determine the effect of UVA exposure (365 nm) on the release of doxorubicin, and therefore, cell viability. Among the different liposome compositions studied by exposing to UVA it was found that PC:CHOL:ATR (7:1:3) formulation responded the best. Antiproliferative effects of all-trans retinal (ATR) and doxorubicin were observedwhen tested on RPE/D407 cell line. Also, the interaction of the cells with doxorubicin and liposomes were studied with flow cytometry and confocal laser scanning microscopy (CLSM). It was found that liposomes or ATR can penetrate the cells while doxorubicin penetrates the nucleus.

Subject Keywords

Liposomes., Doxorubicin., Drug delivery systems.

URI

http://etd.lib.metu.edu.tr/upload/12617733/index.pdf
https://hdl.handle.net/11511/23848

Collections

Graduate School of Natural and Applied Sciences, Thesis

Suggestions

OpenMETU
Core

Biopolymer based micro/nanoparticles as drug carriers for the treatment of skin diseases Eke, Gözde; Hasırcı, Vasıf Nejat; Hasırcı, Nesrin; Department of Micro and Nanotechnology (2011) Controlled drug delivery systems are becoming increasingly interesting with the contribution of nanotechnology. In the case of transdermal applications the greatest limitation is the highly impermeable outermost layer of the skin, the stratum corneum. One promising method of controlled transdermal drug delivery of the skin therapeutics is the use of nanoparticles as carriers. Encapsulation of the drug, as opposed to classical topical application of creams or emulsions, allows the drug to diffuse into hair f...
Peptide-based drug systems / Parlak, Melek; Özçubukçu, Salih; Department of Chemistry (2017) The increasing appeal of safe, cheap and effective treatments against various type of diseases has paved the way for the discovery and development of innovative peptide-based drug and drug delivery systems. The relative ease with which peptide based-materials can be synthesized and the wide range of synthetic techniques available have ensured that these materials can be tuned to adopt specific conformation or modified to contain specific functional groups. Our major focus in this thesis is developing peptid...
UV responsive drug delivery from suprofen incorporated liposomes Demirbağ, Birsen; Hasırcı, Vasıf Nejat; Hasırcı, Nesrin; Department of Biotechnology (2011) Drug delivery systems are designed to achieve low, local doses at the target site. Delivery systems can provide the drug in a continuous manner or in response to environmental stimuli such as temperature, pH or UV. This study aimed to develop photosensitive liposomes that achieve UV-responsive release of their content. The main mechanism was to incorporate a light sensitive molecule into the liposomal bilayer then achieve destabilization of the membrane by exposure to UV. This would result in an on demand r...
Surface functionalization of SBA - 15 particles for amoxicillin delivery Sevimli, Filiz F; Yılmaz, Ayşen; Department of Chemistry (2011) There are several studies in order to control drug delivery, decrease the toxicity of drugs and also for novel biomedical applications. It is necessary to be able to control the release of the drug within the body by using drug delivery systems. Mesoporous silica compounds have only been discovered twenty years ago and they have already attracted many researchers to study these materials for several applications. SBA-15 particles have a highly ordered regular structure and are a good matrix for guest-host a...
Anti-parkinsonian drug delivery across the blood-brain barrier Barçin, Zeynep; Keskin, Dilek; Korkmaz, Seval; Department of Biotechnology (2014) Localized and controlled delivery of drugs at their site of action is necessary to increase its efficiency. Current study was designed on the development of a brain targeted liposomal Levodopa delivery system for the treatment of the Parkinson’s disease. Size and surface charge of the liposomes were modified and optimized to increase the bioavailability and effectiveness of the liposomes. The liposomes were produced in sizes to be administrated intravenously (D: 100-150 nm). In initial optimization studies,...

Citation Formats

S. Heper, “Controlled doxorubicin delivery from photoresponsive liposomes carrying vitamin A derivatives /,” M.S. - Master of Science, Middle East Technical University, 2014.