Ferritin nanocage as a magnetic carrier /

Download

index.pdf

Date

2015

Author

Aslan, Tuğba Nur

Metadata

Show full item record

Item Usage Stats

86
views

24
downloads

There is a large focus on ferritin protein cages due to its biological importance, its behaviour as a useful platform for material synthesis and also providing chemical modification of the exterior surface to create functionality. In this study, iron oxide nanoparticles were synthesized within the cavity of the ferritin (magnetoferritin). Non-radioactive rhenium was incorporated on the protein exterior via conjugation of histidine molecules by using glutaraldehyde crosslinker. 185Re was also bound to magnetoferritin. The rhenium was bound to histidine modified magnetoferritin surface ~ 6 fold more than those bound directly to non-modified magnetoferritin surface and it has the stability of 88% ± 1.8 after 3 days in human blood serum. The toxicity and cellular uptake experiments of prepared biomaterial were tested in MCF-10 (healthy) and MDA-MB-231 (cancerous) cell lines. The biocompatibility is still protected for apoferritin after treatment with cells for 3 days. Thus, utilizing apoferritin holds promise for cancer therapy with a reduced toxicity. The uptake of nanoparticles by the cells increased in 4 hours and cancerous cells exhibited the highest uptake than healthy cells. The effect of iron loadings on relaxivities for MRI application and peroxidase-like activity of magnetoferritin were also investigated. r2 relaxivity and peroxidase-like properties of magnetoferritin showed an increase as the iron loadings increase. The high r2 relaxivity shows that magnetoferritin may serve as T2 contrast agent with high efficiency.

Subject Keywords

Iron oxides., Rhenium., Nanoparticles., Cell-mediated cytotoxicity., Magnetic resonance imaging., Iron oxides

URI

http://etd.lib.metu.edu.tr/upload/12618431/index.pdf
https://hdl.handle.net/11511/24469

Collections

Graduate School of Natural and Applied Sciences, Thesis

Suggestions

OpenMETU
Core

Preparation and characterization of hydrogen-bonded layer-by-layer polymer films containing magnetic nanoparticles Khan, Muhammad Alyaan Ahmed; Erel Göktepe, İrem; Department of Polymer Science and Technology (2014) Stimuli responsive layer-by-layer (LbL) polymer films are promising materials as platforms for controlled release of functional biological molecules such as drugs, proteins, growth hormones, etc. from surfaces. Recently, there has been a growing interest for preparation of LbL polymer platforms containing superparamagnetic iron oxide nanoparticles for dual functionality, i.e. bioimaging and controlled delivery of biological molecules. Moreover, if superparamagnetic iron oxide nanoparticles are embedded into...
Carbon encapsulation of elemental nanoparticles Livan, Pelin; Öztürk, Tayfur; Department of Metallurgical and Materials Engineering (2018) There is a considerable interest in the encapsulation of nanoparticles into the carbon cages for a variety of purposes e.g. providing stability in detrimental environments, preventing agglomeration, controlling surface modifications and improving in electrical conductivity etc. The current study deals with the encapsulation of elemental particles and is made up of two parts. In the first part, the encapsulation of elemental particles was studied using a spark discharge generator where an elemental rod used ...
Applications of the multifunctional magnetic nanoparticles for development of molecular therapies for breast cancer Aşık, Elif; Güray, Tülin; Volkan, Mürvet; Department of Biotechnology (2015) The understanding of how magnetic nanoparticles (MNPs) interact with living system is one of the prerequisite pieces of information needed to be obtained before any further development for desired biomedical applications. In this study, Cobalt Ferrite magnetic nanoparticles (CoFe-MNPs) in their naked and silica-coated forms were characterized. In vitro cell culture for their likely cytotoxicity and genotoxicity potential were examined. The apoptosis, lipid peroxidation, ROS formation and oxidative stress re...
Kinetic approach for the purification of nucleotides with magnetic separation Tural, Servet; Tural, Bilsen; Ece, Mehmet Sakir; Yetkin, Evren; Özkan, Necati (2014-11-01) The isolation of beta-nicotinamide adenine dinucleotide is of great importance since it is widely used in different scientific and technologic fields such as biofuel cells, sensor technology, and hydrogen production. In order to isolate beta-nicotinamide adenine dinucleotide, first 3-aminophenyboronic acid functionalized magnetic nanoparticles were prepared to serve as a magnetic solid support and subsequently they were used for reversible adsorption/desorption of beta-nicotinamide adenine dinucleotide in a...
Polymer Fundamentals: Polymer Synthesis Hasırcı, Vasıf Nejat; Endoğan Tanır, Tuğba; Hasırcı, Nesrin (null, 2017-01-01) Polymers can be of biological and synthetic origin, and are very important in our daily lives. They are used in all the major industries including textile, automotive, household goods, medical devices and products, etc. The significant difference between the requirements of these applications necessitates the availability of a large variety of polymers to choose from. Synthetic polymers can be prepared by addition and condensation polymerization using a variety of polymerization processes to yield polymers ...

Citation Formats

T. N. Aslan, “Ferritin nanocage as a magnetic carrier /,” Ph.D. - Doctoral Program, Middle East Technical University, 2015.