Indigenous hydrocarbon degraders further evaluated for their kerosene degradation and biosurfactant production potentials

Aydın, Dilan Camille
Kerosene, known as jet fuel, is one of the most spilled petroleum product causing serious environmental problems due to recalcitrant compounds found in its structure. The only eco-friendly solution for this problem is bioremediation, in which bacteria are used for the degradation and transformation into non or less toxic forms. The efficiency of this process depends not only on biodegradation ability of the bacterial isolates used but also on their biosurfactant production abilities. Therefore, in this study, 22 previously identified bacterial hydrocarbon degraders were further analyzed for their kerosene degradation and biosurfactant production potentials. Out of 22, 19 bacterial isolates were found to utilize kerosene after pre-selection. The degradation abilities of the pre-selected isolates were determined chromatographically and 7 isolates namely; Pseudomonas plecoglossicida Ag10, Staphylococcus aureus Ba01, Stenetrophomonas rhizophila Ba11, Delftia acidovorans Cd11, Acinetobacter calcoaceticus Fe10, Pseudomonas koreensis Hg11 and Acinetobacter johnsonii Sb01 were stood out as efficient kerosene degraders with degradation abilities in between 69-84%. All the efficient degraders were showed to harbor the alkB gene responsible for kerosene degradation through the polymerase chain reaction (PCR) analyses. Biosurfactant production abilities of 19 kerosene degraders were also tested and Pseudomonas plecoglossicida Ag10, Raoultella planticola Ag11, Staphylococcus aureus Ba01, Enterococcus faecalis Cr07, Acinetobacter johnsonii Sb01 and Pantoea agglomerans Sn11 were determined as biosurfactant producers through oil spreading activity, emulsification index and microbial adhesion to hydrocarbon tests. Blue agar plate method, thin layer chromatography and fourier transform infrared spectroscopy analysis were used to characterize the biosurfactants. The results revealed that, glycolipid type rhamnolipids were majoring in kerosene degraders. The gene responsible for rhamnolipid biosynthesis, rhlAB, was also shown in all the rhamnolipid producers by PCR analysis.


Effect of chromium on the oxidation of steels used in the construction of petroleum refinery heaters
Sultan, Abdelrahman Saleh; Karakaya, İshak; Department of Metallurgical and Materials Engineering (2013)
Low chromium steels used in the construction of petroleum refinery heaters work in flue gas environments containing water vapor. On the other hand, the higher oxidation rates of chromium containing steels in moist atmospheres have been reported. P-5, P-22, P-11(containing 5, 2.25 and 1.25 %Cr) and C-5 steels, used in petroleum refinery construction, were subjected to isothermal oxidation tests. These steels were tested in two different environments; air and flue gas of natural gas combustion -CO2+2H2O+7.52N...
Screening of biosurfactant producing and diesel oil degrading bacteria from petroleum hydrocarbon contaminated surface waters
Onur, Gözde; İçgen, Bülent; Department of Environmental Engineering (2015)
Hydrocarbon contamination may happen in various ways such as accidents during fuel transportation by trucks and ships, leakage of oil from underground storage tanks, or during extraction and processing of oil. These contaminations can be treated by several methods including physical, chemical and biological treatment. During biological cleaning up, hydrocarbon-degrading bacteria emulsifying hydrocarbons by producing biosurfactants are used. Therefore, isolation and identification of biosurfactant producing ...
Cimino, S.; Allouis, Christophe Gerard; Mancino, G.; Nigro, R. (2014-05-01)
Hybrid catalytic combustion technology, which is a staged process comprising a preliminary catalytic partial oxidation (CPO) step followed by a homogeneous flame combustion with interstage heat removal, was tested for the first time with mixtures of methane and hydrogen. Short contact time CPO experiments were run to elucidate the effect of the progressive substitution of methane with H-2 in the fuel feed to the structured catalytic reactor, which was operated under self-sustained conditions at high tempera...
Investigation on indigenous bacteria for individual BTEX degradation potentials and relative pathways used
Yavaş, Alper; İçgen, Bülent; Parlaktuna, Mahmut; Department of Biotechnology (2018)
Monoaromatic hydrocarbons including benzene, toluene, ethylbenzene and xylene collectively called as BTEX are found in the composition of crude oil and gasoline as an additive and thought to be the most serious contaminants of soil and groundwater. It is expected that indigenous bacteria isolated from petroleum hydrocarbon contaminated sites probably have degradation potential for the BTEX compounds. In this study, out of 22, 19 bacterial strains were selected as potential degraders for at least one of the ...
Air and oxy-fuel combustion behaviour of petcoke/lignite blends
Yuzbasi, Nur Sena; Selçuk, Nevin (2012-02-01)
The pyrolysis and combustion behaviour of a petroleum coke (petcoke), an indigenous lignite and their 70/30 wt.% blend in air and oxy-fuel conditions were investigated by using non-isothermal thermogravimetric method (TGA) coupled with Fourier transform infrared (FTIR) spectrometer. Blend samples were prepared by mixing lignite, which has low calorific value, high ash and moisture contents with petcoke that has high calorific value, low ash and moisture content, in the proportion of 70:30. Pyrolysis tests w...
Citation Formats
D. C. Aydın, “Indigenous hydrocarbon degraders further evaluated for their kerosene degradation and biosurfactant production potentials,” M.S. - Master of Science, Middle East Technical University, 2018.