Genetic composition of four marginally located Anatolian black pine (Pinus nigra subsp. pallasiana) populations determined by SSR markers

Aygün, Sıla
Anatolian black pine (Pinus nigra Arnold subsp. pallasiana (Lamb.) Holmboe) is one of the most economically and ecologically important coniferous tree species of Turkey. Global climate change is obviously going to affect distribution and development of forest tree species and the marginal populations will be the most vulnerable ones. Since these populations are located in outer borders of their natural distribution area, they are expected to have original genetic makeup shaped by unsuitable living conditions. To predict the future of forest ecosystems, it is important to determine the genetic composition and adaptation processes on lifecycle of these populations. In this thesis, genetic makeup of 4 Anatolian black pine marginal populations were studied in 3 different life stages (seed, seedling and mature stages). Totally 720 genotypes were investigated by means of 10 microsatellite DNA (SSR: single sequence repeats) markers. It was found that, heterozygosity values were low (Ho: 0.22±0.01) and level of genetic differentiation was high (FST: 0.13±0.03) among populations. In addition, it was determined how natural selection and fitness affect these populations’ genetic diversity in different life stages. Obtained data showed that genetic diversity gradually diminished after natural selection from seed to mature stages. Among populations in different life stages, seed stage of Beynam population possessed the highest diversity level (Ho: 0.26±.0.04). Hence, it is advised that when afforestation or conservation activities dealing with marginal black pine populations , genetic diversity patterns at the three stages of marginal populations should be considered to improve adaptation of plantations to changing adverse environmental conditions


Marjinal Karaçam (Pinus nigra Subsp. Pallasiana) Populasyonlarında Genetik Çeşitlilik ve Akrabalık Düzeyi: Bu Tür Populasyonlarla Yapılacak Ağaçlandırmaların Genetik Uyum Potansiyeli
Kaya, Zeki; Çengel, Burcu; Aygün, Sıla(2018-04-15)
Anadolu karaçamı (Pinus nigra Arnold subsp. pallasiana (Lamb.) Holmboe) geniş yayılışı ile ekonomik ve ekolojik açıdan ülkemizin en önemli ibreli orman ağacı türlerinden birisidir. İç Anadolu steplerinde kalıntı orman olarak kalan marjinak karaçam populasyonları değişen iklim koşullarından en fazla etkilenecek populasyonlar olduğu düşünülmektedir. Çünkü bu populasyonlar, türün yetişme alanının uç sınırlarında yer alır ve orijinal bir genetik yapıya sahip oldukları varsayılır. Orman ekosistemlerinin geleceği...
The evolutionary divergence of Pinus nigra subsp pallasiana and its varieties based on noncoding trn regions of chloroplast genome
Gulsoy, Aysun Demet; Gulsoy, Ali Murat; Cengel, Burcu; Kaya, Zeki (2014-01-01)
The Anatolian black pine [Pinus nigra Arnold subsp. pallasiana (Lamb.) Holmboe var. pallasiana] is one of the subspecies of European black pine, growing naturally as a widespread mid-elevation species in the southern, western, and northern Anatolian Mountains of Turkey. Three well-recognized varieties [var. pallasiana, var. fastigiata Businsky with pyramidal form, and var. seneriana (Saatcioglu) Yalt. with globular-shaped crown with multiple stems] occur naturally, but the studies on them are very limited. ...
Genetic characterization pinus nigra subspecies pallasiana varieties, natural populations (seed stands), seed orchards and plantations
Çengel (Nazlıer), Burcu; Kaya, Zeki; Department of Biology (2005)
Pinus nigra subsp. pallasiana is one of the most widespread and economically important forest tree species in Turkey. Primary objective of the present study was to to reveal the effects of forestry practices by determining genetic diversity of natural and managed seed sources by means of RAPD markers. Secondly, two varieties were also investigated to reveal their pattern of genetic variation. Seed stands, seed orchards and plantations were screened against 11 RAPD primers and generated 152 polymorphic DNA l...
Genetic relationships among perennial and annual Cicer species growing in Turkey as revealed by allozymes
Sudupak, MA; Kence, Aykut (Springer Science and Business Media LLC, 2004-05-01)
Allozyme polymorphisms were used to assess genetic variation and relationships among ten Cicer species ( annuals and perennials) growing in Turkey. Using seven enzyme systems, 12 putative scorable loci were detected and surveyed for polymorphism in an accession collection including wild and cultivated forms. Variation was generally low within accessions and species, but common between species. Cluster analysis based on the pairwise genetic distance coefficients (Nei 1978) among accessions and species using ...
Genetic variation in Turkish red pine (Pinus brutia Ten.) seed stands as determined by RAPD markers
Kandedmir, GE; Kandemir, I; Kaya, Zeki (2004-01-01)
Turkish red pine (Pinus brutia Ten.) is one of the important tree species in Turkey. The species has been heavily disturbed by anthropogenic factors and fire, making it important to understand the magnitude of the impact on genetic diversity of areas reserved for seed production. To explore the extent of genetic variation in Turkish red pine seed stands, a random amplified polymorphic DNA (RAPD) marker system was used in the study. The estimated genic diversity for seed stands was found to range from H=0.17...
Citation Formats
S. Aygün, “Genetic composition of four marginally located Anatolian black pine (Pinus nigra subsp. pallasiana) populations determined by SSR markers,” M.S. - Master of Science, Middle East Technical University, 2018.