Effect of inactivation of genes involved in ammonium regulation on the biohydrogen production of Rhodobacter capsulatus

Date

2011-10-01

Author

Pekgoz, Gulsah
Gündüz, Ufuk
Eroglu, Inci
Yucel, Meral
Kovacs, Kornel
Rakhely, Gabor

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

88
views

0
downloads

Hydrogen production by nitrogenase is an energetically expensive process for the cell, hence strictly controlled at different levels. Ammonium is one of the substances regulating nitrogenase activity. The key proteins in the regulation of nitrogenase by ammonium are two regulatory proteins; GlnB and GlnK. In order to increase hydrogen production of Rhodobacter capsulatus DSM1710 (wild type strain) grown on agricultural materials/wastes, ammonium inhibition of nitrogenase enzyme has to be eliminated. In this study, GlnB and GlnK were targeted to be inactivated by in frame site-directed mutagenesis. The glnB mutant R. capsulatus (GP1 strain) was obtained at the end of mutagenesis studies. In the case of glnK, the suicide vector was constructed and delivered into the cells. However, glnK mutant could not be obtained.

Subject Keywords

Rhodobacter capsulatus, Biohydrogen, GlnB/GlnK proteins, Ammonium inhibition, Site-directed mutagenesis, Kinetic modelling

URI

https://hdl.handle.net/11511/29909

Journal

INTERNATIONAL JOURNAL OF HYDROGEN ENERGY

DOI

https://doi.org/10.1016/j.ijhydene.2011.07.123

Collections

Graduate School of Natural and Applied Sciences, Article

Suggestions

OpenMETU
Core

EFFECT OF TANNIC ACID ON GLUTATHIONE S-TRANSFERASE AND NAD(P)H: QUINONE OXIDOREDUCTASE 1 ENZYMES IN RABBIT LIVER AND KIDNEY KARAKURT, SERDAR; Adalı, Orhan (2011-01-01) Glutathione S-transferase and NAD(P)H: quinone oxidoreductase 1 enzymes have important roles in detoxification and also activation of a wide variety of chemicals. Moreover, resistance of tumor cells against chemotherapeutic agents has been implicated in GST activities. The aim of this study was to investigate the effect of polyphenolic compound tannic acid on rabbit liver and kidney glutathione S-transferase and NAD(P)H: quinone oxidoreductase 1 enzyme activities. Tannic acid was found to be a potent inhibi...
Significance of carbon to nitrogen ratio on the long-term stability of continuous photofermentative hydrogen production Androga, Dominic Deo; Ozgur, Ebru; Eroglu, Inci; Gündüz, Ufuk; Yucel, Meral (2011-12-01) The stable and optimized operation of photobioreactors (PBRs) is the most challenging task in photofermentative biological hydrogen production. The carbon to nitrogen ratio (C/N) in the feed is a critical parameter that significantly influences microbial growth and hydrogen production. In this study, the effects of changing the C/N ratio to achieve stable biomass and continuous hydrogen production using fed-batch cultures of Rhodobacter capsulatus YO3 (uptake hydrogenase deleted, hup-) were investigated. Th...
Potential use of thermophilic dark fermentation effluents in photofermentative hydrogen production by Rhodobacter capsulatus Ozgur, Ebru; Afsar, Nilufer; de Vrije, Truus; Yucel, Meral; Gündüz, Ufuk; Claassen, Pieternel A. M.; Eroglu, Inci (2010-01-01) Biological hydrogen production by a sequential operation of dark and photofermentation is a promising route to produce hydrogen. The possibility of using renewable resources, like biomass and agro-industrial wastes, provides a dual effect of sustainability in biohydrogen production and simultaneous waste removal. In this study, photofermentative hydrogen production on effluents of thermophilic dark fermentations on glucose, potato steam peels (PSP) hydrolysate and molasses was investigated in indoor, batch ...
Effect of Different Homogenization Techniques on the Particle Size of Liposomes and Storage Characterization by NMR Relaxometry Güner, Selen; Öztop, Halil Mecit (null; 2015-08-12) Liposomes are double-layered spherical vesicles made up of polar lipids and could be used in pharmaceutical, personal care, chemical and food industries to encapsulate both hydrophobic and hydrophilic compounds. Being biodegradable, biocompatible not having any toxic effects and having the ability to release the active agents when desired make these systems advantageous for many applications. The main natural sources used for liposomes are egg and soy. In this study, egg and soy lecithin with higher purity ...
Effect of pectin methyl esterase (PME) and CaCl2 infusion on the cell integrity of fresh-cut and frozen-thawed mangoes: An NMR relaxometry study Kırtıl, Emrah; Öztop, Halil Mecit; Barrett, Diane M.; McCarthy, Michael J. (Elsevier BV, 2014-12) This study describes the use of Nuclear Magnetic Resonance (NMR) relaxometry to understand the effect of pectin methyl esterase (PME) + CaCl2 infusion under different conditions on cell integrity of fresh-cut and frozen-thawed mangoes. Infusion experiments were performed at: atmospheric pressure, vacuum conditions of 50 kPa and 10 kPa. For NMR relaxometry experiments T-2 (spin-spin relaxation time), T-1 (spin-lattice-relaxation times) and 2D T-1-T-2 experiments were performed. Results showed that, as the se...

Citation Formats

G. Pekgoz, U. Gündüz, I. Eroglu, M. Yucel, K. Kovacs, and G. Rakhely, “Effect of inactivation of genes involved in ammonium regulation on the biohydrogen production of Rhodobacter capsulatus,” INTERNATIONAL JOURNAL OF HYDROGEN ENERGY, pp. 13536–13546, 2011, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/29909.