Effect of inactivation of genes involved in ammonium regulation on the biohydrogen production of Rhodobacter capsulatus

Date

2011-10-01

Author

Pekgoz, Gulsah
Gündüz, Ufuk
Eroglu, Inci
Yucel, Meral
Kovacs, Kornel
Rakhely, Gabor

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Hydrogen production by nitrogenase is an energetically expensive process for the cell, hence strictly controlled at different levels. Ammonium is one of the substances regulating nitrogenase activity. The key proteins in the regulation of nitrogenase by ammonium are two regulatory proteins; GlnB and GlnK. In order to increase hydrogen production of Rhodobacter capsulatus DSM1710 (wild type strain) grown on agricultural materials/wastes, ammonium inhibition of nitrogenase enzyme has to be eliminated. In this study, GlnB and GlnK were targeted to be inactivated by in frame site-directed mutagenesis. The glnB mutant R. capsulatus (GP1 strain) was obtained at the end of mutagenesis studies. In the case of glnK, the suicide vector was constructed and delivered into the cells. However, glnK mutant could not be obtained.

Subject Keywords

Rhodobacter capsulatus, Biohydrogen, GlnB/GlnK proteins, Ammonium inhibition, Site-directed mutagenesis, Kinetic modelling

URI

https://hdl.handle.net/11511/29909

Journal

INTERNATIONAL JOURNAL OF HYDROGEN ENERGY

DOI

https://doi.org/10.1016/j.ijhydene.2011.07.123

Collections

Graduate School of Natural and Applied Sciences, Article

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Citation Formats

G. Pekgoz, U. Gündüz, I. Eroglu, M. Yucel, K. Kovacs, and G. Rakhely, “Effect of inactivation of genes involved in ammonium regulation on the biohydrogen production of Rhodobacter capsulatus,” INTERNATIONAL JOURNAL OF HYDROGEN ENERGY, pp. 13536–13546, 2011, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/29909.