Effect of Different Homogenization Techniques on the Particle Size of Liposomes and Storage Characterization by NMR Relaxometry

Date

2015-08-12

Author

Güner, Selen
Öztop, Halil Mecit

Metadata

Show full item record

Item Usage Stats

188
views

0
downloads

Liposomes are double-layered spherical vesicles made up of polar lipids and could be used in pharmaceutical, personal care, chemical and food industries to encapsulate both hydrophobic and hydrophilic compounds. Being biodegradable, biocompatible not having any toxic effects and having the ability to release the active agents when desired make these systems advantageous for many applications. The main natural sources used for liposomes are egg and soy. In this study, egg and soy lecithin with higher purity (>70%) and a commercially sold soy lecithin was used to determine the effect of different homogenization methods on the particle size of liposomes. Microfluidization, ultrasonic probe and ultrasonic bath methods were used. Microfluidization gave the smallest particle size results. Prehomogenization speed after 20, 000 rpm is found to have no significant effect on final particle size (p<0.05). Effects of microfluidization pressure and solution type on particle size were also investigated. Trials were conducted between 300- 1300 bars and either distilled water (pH≈7) or acetate buffer (pH≈3.8). Effect of solution type on particle size was found significant. For the long term stability, the samples were stored at refrigerator and room temperatures (4oC & 25oC) and NMR T2 Relaxometry experiments were conducted during storage. The T2 values decreased during storage. Liposomes prepared from commercial lecithin had the lowest T2, supporting the idea that it was not pure and had higher concentration of lipids.

URI

https://hdl.handle.net/11511/81308
https://www.longdom.org/proceedings/effect-of-different-homogenization-techniques-on-the-particle-size-of-liposomes-and-storage-characterization-by-nmr-relaxometry-28580.html

Conference Name

Effect of Different Homogenization Techniques on the Particle Size of Liposomes and Storage Characterization by NMR Relaxometry", 4th International Conference and Exhibition on Food Processing Technology, Londra, İngiltere, 10 - 12 Ağustos 2015

Collections

Department of Food Engineering, Conference / Seminar

Suggestions

OpenMETU
Core

Formation and characterization of food grade liposome systems Güner, Selen; Öztop, Halil Mecit; Department of Food Engineering (2015) Liposomes are double-layered spherical vesicles made up of polar lipids and could be used in pharmaceutical, personal care, chemical and food industries to encapsulate both hydrophobic and hydrophilic compounds. Being biodegradable, biocompatible not having any toxic effects and having the ability to release the active agents when desired make these systems advantageous for many applications. The main natural sources used for liposome formation are egg and soy. In this study, egg and soy lecithin with highe...
Effect of nutrients on culture dynamics of marine phytoplankton Eker-Develi, E; Kıdeyş, Ahmet Erkan; TUĞRUL, SÜLEYMAN (2006-01-01) The effects of nitrate, ammonium and phosphate on the abundance, chlorophyll a content (chl a), in vivo fluorescence, particulate organic carbon, nitrogen, phosphorus and cell morphology of the diatom Skeletonema costatum, the dinoflagellate Prorocentrum micans and the coccolithophore Emiliania huxleyi were investigated in the laboratory. The carbon:chlorophyll a ratio (as weight), a parameter often used in productivity estimates, differed substantially among the three species as well as at different nutrie...
Effect of film deposition conditions on the properties of multilayers of poly[2-(dimethylamino)ethyl methacrylate-b-poly(2(diethylamino)ethyl methacrylate)] micelles and poly(sodium 4-styrenesulfonate) Gündoğdu, Dilara; Erel Göktepe, İrem; Department of Chemistry (2017) Block copolymer micelles (BCMs) are ideal drug carriers due to their hydrophobic core regions which can encapsulate water insoluble drug molecules. BCMs with stimuli-responsive cores are of specific interest due to release of the cargo by simply changing the environmental conditions, e.g. pH, temperature, ionic strength. Incorporation of BCMs into layer-by-layer (LbL) films is important to keep the BCMs stable in bloodstream and prevent the release of the cargo at an early stage. In addition, BCMs increase ...
Effect of Alkyl Derivatization on Several Properties of N-Halamine Antimicrobial Siloxane Coatings Kocer, Hasan B.; Akdağ, Akın; Ren, Xuehong; Broughton, R. M.; Worley, S. D.; Huang, T. S. (American Chemical Society (ACS), 2008-10-01) Variation of alkyl substitution at position 5 on the hydantoin ring of a series of N-halamine siloxane derivatives has been employed to better understand the biocidal activities of these compounds for use in preparing antimicrobial coatings. The alkyl derivatization of the hydantoin ring at its 5 position, while an essentially constant chlorine loading is maintained at the I position, has shown that there is little dependence of the antimicrobial efficacy against Escherichia coli O157:H7 on the alkyl chain ...
Effect of protein aggregation in the aqueous phase on the binding of membrane proteins to membranes Doebler, R; Basaran, N; Goldston, H; Holloway, PW (Elsevier BV, 1999-02-01) Analysis of the binding of hydrophobic peptides or proteins to membranes generally assumes that the solute is monomeric in both the aqueous phase and the membrane. Simulations were performed to examine the effect of solute self-association in the aqueous phase on the binding of monomeric solute to lipid vesicles. Aggregation lowered the initial concentration of monomeric solute, which was then maintained at a relatively constant value at the expense of the aggregated solute, as the lipid concentration was i...

Citation Formats

S. Güner and H. M. Öztop, “Effect of Different Homogenization Techniques on the Particle Size of Liposomes and Storage Characterization by NMR Relaxometry,” presented at the Effect of Different Homogenization Techniques on the Particle Size of Liposomes and Storage Characterization by NMR Relaxometry”, 4th International Conference and Exhibition on Food Processing Technology, Londra, İngiltere, 10 - 12 Ağustos 2015, Londra, İngiltere, 2015, Accessed: 00, 2021. [Online]. Available: https://hdl.handle.net/11511/81308.