In vivo application of biodegradable controlled antibiotic release systems for the treatment of implant-related osteomyelitis

Gursel, I
Korkusuz, F
Turesin, F
Alaeddinoglu, NG
Hasırcı, Vasıf Nejat
In this study the construction and in vivo testing of antibiotic-loaded polyhydroxyalkanoate rods were planned for use in the treatment of implant-related osteomyelitis. The rods were constructed of poly(3-hydroxybutyrare-co-3-hydroxyvalerate) and poly(3hydroxybutyrate-co-4-hydroxybutyrate), carrying 50% (w/w) Sulperazone(R) or Duocid(R). They were implanted in rabbit tibia in which implant-related osteomyelitis (IRO) had been induced with Staphylococcus aureus. The effectiveness of the antibiotics in the treatment of IRO was determined. The establishment of IRO with bacterial inoculation was complete after 3 weeks with 100% infection rate in all groups. There was no contamination or super-infection. Both antibiotics were found to be highly effective against the bacteria. Following the application of Sulperazone-P(3-HB-co-4-HB) rods, no infective agents could be isolated from the infection site within the 6-week test period, indicating complete treatment of the infection. Macroscopical evaluation at follow-up revealed no drainage, minimal swelling and increase in local warmth, most probably due to the surgery rather than to a reaction towards the implant. The overall scores for radiological findings by the end of 6 weeks were 0.8/5 for the antibiotic-loaded rod implanted in the right limb, and 1.1/5 For the antibiotic-free rod implanted in the left limb. There was no statistical difference between the antibiotic-loaded and antibiotic-free polymeric rods. In vivo drug release was almost complete within the first week. One interesting observation, however, was that the therapy was still very effective even when the release rate was very high. In the SEM of in vitro tested rods, the polymeric component was unchanged in 2 weeks while the drug leached out, leaving voids behind. In vivo, however, the morphology of the implant was significantly modified within 6 weeks post-implantation. Since a substantial degree of the in vivo drug release was complete within 1 week, we believe that dissolution of the drug must be the predominant mechanism through which the drug release is controlled.


In vivo response to biodegradable controlled antibiotic release systems
Korkusuz, F; Korkusuz, P; Eksioglu, F; Gursel, I; Hasırcı, Vasıf Nejat (2001-05-01)
In this study, the major goal was to evaluate in vitro and in vivo findings by macroscopy, radiology, and histology to determine the effectiveness of therapy of experimental implant-related osteomyelitis with antibiotic carrier rods constructed of microbial polyesters. The polymers used were poly(3-hydroxybutyrate-co-4-hydroxyvalerate) [P(3HB-co-4-HB)] and poly(3-hydroxybutyrate-co-3-hydroxy-valerate) [P(3-HB-co-3-HV)]. Both the Sulperazone(R) and the Duocid(R)-P(3-HB-co-4-HB) rods with a drug to polymer ra...
Biodegradable polyhydroxyalkanoate implants for osteomyelitis therapy: in vitro antibiotic release
Turesin, F; Gursel, I; Hasırcı, Vasıf Nejat (2001-01-01)
Various random copolyesters of 3-hydroxybutyrate and 3-hydroxyvalerate (PHBV) and 3-hydroxybutyrate and l-hydroxybutyrate P(3HB-4HB) were used in the construction of biodegradable, implantable rods for the local delivery of antibiotics (Sulperazone(R) and Duocid(R)) in chronic osteomyelitis therapy. Drug loading, type of active agent, and additional coating of the implant surface all have significant contributions to the in vitro release profile. The rate and duration of Sulperaxone(R) release from P(3HB-4H...
In vitro and transdermal penetration of PHBV micro/nanoparticles
Eke, G.; Kuzmina, A. M.; Goreva, A. V.; Shishatskaya, E. I.; Hasırcı, Nesrin; Hasırcı, Vasıf Nejat (2014-06-01)
The purpose of this study was to develop micro and nano sized drug carriers from poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV), and study the cell and skin penetration of these particles. PHBV micro/nanospheres were prepared by o/w emulsion method and were stained with a fluorescent dye, Nile Red. The particles were fractionated by centrifugation to produce different sized populations. Topography was studied by SEM and average particle size and its distribution were determined with particle sizer. Cel...
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Kenar, Halime; Hasırcı, Vasıf Nejat; Toner, Mehmet; Department of Biotechnology (2003)
This study aimed at guiding osteoblast cells on biodegradable polymer carriers with well-defined surface microtopography and chemistry, and investigating the effect of cell alignment on osteoblast phenotype expression. A blend of two different polyesters, one being natural in origin (PHBV) and the other synthetic (P(L/DL)LA), was used to form a film with parallel macro- (250 um wide) or microgrooves (27 jam wide) on its surface, by solvent casting on patterned templates. The micropatterned Si template was p...
In vitro induction of growth and development of common juniper(Juniperus communis L.) from shoot and bud explants
Koçer, Zeynep Ahsen; Kaya, Zeki; Department of Biotechnology (2005)
The objective of the study was to investigate the optimum conditions for in vitro regeneration of common juniper (Juniperus communis L.) by using indirect organogenesis approach. Throughout the study; callus induction, organogenesis, improved organogenesis and root induction experiments were performed sequentially. It was found that explant position, genotype, gender, treatments and sampling time had significant effects on callus induction rate in common juniper. The results of treatments indicated that IBA...
Citation Formats
I. Gursel, F. Korkusuz, F. Turesin, N. Alaeddinoglu, and V. N. Hasırcı, “In vivo application of biodegradable controlled antibiotic release systems for the treatment of implant-related osteomyelitis,” BIOMATERIALS, pp. 73–80, 2001, Accessed: 00, 2020. [Online]. Available: