Feasibility of a porcine oral mucosa equivalent: A preclinical study

Date

2012-08-01

Author

Kinikoglu, Beste
Hemar, Julie
Hasırcı, Vasıf Nejat
Breton, Pierre
Damour, Odile

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

184
views

0
downloads

Oral tissue engineering aims to treat and fill tissue deficits caused by congenital defects, facial trauma, or malignant lesion surgery, as well as to study the biology of oral mucosa. The Food and Drug Administration (FDA) and the European Medicines Agency (EMA) require a large animal model to evaluate cell-based devices, including tissue-engineered oral mucosa, prior to initiating human clinical studies. Porcine oral mucosa is non-keratinized and resembles that of humans more closely than any other animal in terms of structure and composition; however, there have not been any reports on the reconstruction of a porcine oral mucosa equivalent, probably due to the difficulty to culture porcine fibroblasts. In this study, we demonstrate the feasibility of a 3D porcine oral mucosa equivalent based on a collagen-GAG-chitosan scaffold, as well as reconstructed porcine epithelium by using an amniotic membrane as support, or without any support in form of epithelial cell sheets by using thermoresponsive culture plates. Explants technique was used for the isolation of the porcine fibroblasts and a modified fibroblast medium containing 20% fetal calf serum was used for their culture. The histological and transmission electron microscopic analyses of the resulting porcine oral mucosa models showed the presence of non-keratinized epithelia expressing keratin 13, the major differentiation marker of non-keratinized oral mucosa, in all models, and the presence of newly synthesized collagen fibers in the lamina propria equivalent of the full-thickness model, indicating the functionality of porcine fibroblasts.

Subject Keywords

Porcine oral mucosa equivalent, Oral tissue engineering, 3D oral mucosa model, Reconstructed epithelium, Epithelial cell sheet, Fibroblasts, Epithelial cells

URI

https://hdl.handle.net/11511/30456

Journal

ARTIFICIAL CELLS BLOOD SUBSTITUTES AND BIOTECHNOLOGY

DOI

https://doi.org/10.3109/10731199.2011.644293

Collections

Graduate School of Natural and Applied Sciences, Article

Suggestions

OpenMETU
Core

RECONSTRUCTION OF A FULL-THICKNESS COLLAGEN-BASED HUMAN ORAL MUCOSAL EQUIVALENT Kinikoglu, B.; Auxenfans, C.; Pierrillas, P.; Burillon, C.; Hasırcı, Vasıf Nejat; Damour, O. (2009-07-01) Tissue engineered human oral mucosa has the potential to be applied to the closure of surgical wounds after tissue deficits due to facial trauma, malignant lesion surgery or preposthetic procedure. It can also be used to elucidate the biology and pathology of oral mucosa and as a model alternative to animals for safety testing of oral care products. Using the technology previously developed in our laboratory for the production of a skin equivalent, we were able to reconstruct a nonkeratinized full-thickness...
Tissue engineering of full-thickness human oral mucosa Kınıkoğlu, Beste; Hasırcı, Vasıf Nejat; Damour, Odile; Department of Biotechnology (2010) Tissue engineered human oral mucosa has the potential to fill tissue deficits caused by facial trauma or malignant lesion surgery. It can also help elucidate the biology of oral mucosa and serve as an alternative to in vivo testing of oral care products. The aim of this thesis was to construct a tissue engineered full-thickness human oral mucosa closely mimicking the native tissue. To this end, the feasibility of the concept was tested by co-culturing fibroblasts and epithelial cells isolated from normal hu...
Tissue engineering of oral mucosa: a shared concept with skin Kinikoglu, Beste; Damour, Odile; Hasırcı, Vasıf Nejat (2015-03-01) Tissue-engineered oral mucosa, in the form of epithelial cell sheets or full-thickness oral mucosa equivalents, is a potential solution for many patients with congenital defects or with tissue loss due to diseases or tumor excision following a craniofacial cancer diagnosis. In the laboratory, it further serves as an in vitro model, alternative to in vivo testing of oral care products, and provides insight into the behavior of the oral mucosal cells in healthy and pathological tissues. This review covers the...
Investigation of surface structure and biocompatibility of chitosan-coated zirconia and alumina dental abutments Kalyoncuoglu, Ulku Tugba; Yilmaz, Bengi; Koc, Serap Gungor; Evis, Zafer; ARPACI, PEMBEGÜL UYAR; Kansu, Gulay (Wiley, 2018-12-01) Background: For long-term success of dental implants, it is essential to maintain the health of the surrounding soft tissue barrier, which protects the bone-implant interface from the microorganisms. Although implants based on titanium and its alloys still dominate the dental implant market, alumina (Al2O3) and zirconia (ZrO2) implant systems are widely used in the area. However, they provide smooth and bioinert surfaces in the transmucosal region, which poorly integrate with the surrounding tissues.
Characterization and identification of human mesenchymal stem cells at molecular level Aksoy, Ceren; Severcan, Feride; Çetinkaya, Duygu Uçkan; Department of Biotechnology (2012) Bone marrow mesenchymal stem cells (BM-MSCs) are pluripotent cells that can differentiate into a variety of non-hematopoietic tissues. They also maintain healthy heamatopoiesis by providing supportive cellular microenvironment into BM. In this thesis, MSCs were characterized in terms of their morphological, immunophenotypical and differentiation properties. Then, they were examined by attenuated total reflection-Fourier transform infrared (ATR-FTIR) spectroscopy together with hierarchical clustering, and FT...

Citation Formats

B. Kinikoglu, J. Hemar, V. N. Hasırcı, P. Breton, and O. Damour, “Feasibility of a porcine oral mucosa equivalent: A preclinical study,” ARTIFICIAL CELLS BLOOD SUBSTITUTES AND BIOTECHNOLOGY, pp. 271–274, 2012, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/30456.