RECONSTRUCTION OF A FULL-THICKNESS COLLAGEN-BASED HUMAN ORAL MUCOSAL EQUIVALENT

2009-07-01
Kinikoglu, B.
Auxenfans, C.
Pierrillas, P.
Burillon, C.
Hasırcı, Vasıf Nejat
Damour, O.
Tissue engineered human oral mucosa has the potential to be applied to the closure of surgical wounds after tissue deficits due to facial trauma, malignant lesion surgery or preposthetic procedure. It can also be used to elucidate the biology and pathology of oral mucosa and as a model alternative to animals for safety testing of oral care products. Using the technology previously developed in our laboratory for the production of a skin equivalent, we were able to reconstruct a nonkeratinized full-thickness human oral mucosal equivalent closely mimicking human native oral mucosa. The successive coculture of human lamina propria fibroblasts and human oral epithelial cells isolated from the nonkeratinized region of oral cavity in a porous collagen–glycosaminoglycan (GAG)–chitosan scaffold gave rise to a lamina propria equivalent (LPE) and then to an oral mucosa equivalent (OME). The results of the histology, immunohistology and transmission electron microscopy of this OME demonstrated the presence of a nonkeratinized pluristratified and differentiated epithelium as in native nonkeratinized human oral mucosa expressing both K13 and K3/76. This epithelium was firmly anchored to the LPE by a continuous and ultrastructurally well-organized basement membrane. In the LPE, fibroblasts synthesized new extracellular matrix where the average collagen fibre diameter was 28.4 nm, close to that of native oral mucosa. The proliferative capacity of the basal cells was demonstrated by the expression of Ki67
INTERNATIONAL JOURNAL OF ARTIFICIAL ORGANS

Suggestions

Tissue engineering of full-thickness human oral mucosa
Kınıkoğlu, Beste; Hasırcı, Vasıf Nejat; Damour, Odile; Department of Biotechnology (2010)
Tissue engineered human oral mucosa has the potential to fill tissue deficits caused by facial trauma or malignant lesion surgery. It can also help elucidate the biology of oral mucosa and serve as an alternative to in vivo testing of oral care products. The aim of this thesis was to construct a tissue engineered full-thickness human oral mucosa closely mimicking the native tissue. To this end, the feasibility of the concept was tested by co-culturing fibroblasts and epithelial cells isolated from normal hu...
Feasibility of a porcine oral mucosa equivalent: A preclinical study
Kinikoglu, Beste; Hemar, Julie; Hasırcı, Vasıf Nejat; Breton, Pierre; Damour, Odile (2012-08-01)
Oral tissue engineering aims to treat and fill tissue deficits caused by congenital defects, facial trauma, or malignant lesion surgery, as well as to study the biology of oral mucosa. The Food and Drug Administration (FDA) and the European Medicines Agency (EMA) require a large animal model to evaluate cell-based devices, including tissue-engineered oral mucosa, prior to initiating human clinical studies. Porcine oral mucosa is non-keratinized and resembles that of humans more closely than any other animal...
Investigation of surface structure and biocompatibility of chitosan-coated zirconia and alumina dental abutments
Kalyoncuoglu, Ulku Tugba; Yilmaz, Bengi; Koc, Serap Gungor; Evis, Zafer; ARPACI, PEMBEGÜL UYAR; Kansu, Gulay (Wiley, 2018-12-01)
Background: For long-term success of dental implants, it is essential to maintain the health of the surrounding soft tissue barrier, which protects the bone-implant interface from the microorganisms. Although implants based on titanium and its alloys still dominate the dental implant market, alumina (Al2O3) and zirconia (ZrO2) implant systems are widely used in the area. However, they provide smooth and bioinert surfaces in the transmucosal region, which poorly integrate with the surrounding tissues.
Tissue engineering of oral mucosa: a shared concept with skin
Kinikoglu, Beste; Damour, Odile; Hasırcı, Vasıf Nejat (2015-03-01)
Tissue-engineered oral mucosa, in the form of epithelial cell sheets or full-thickness oral mucosa equivalents, is a potential solution for many patients with congenital defects or with tissue loss due to diseases or tumor excision following a craniofacial cancer diagnosis. In the laboratory, it further serves as an in vitro model, alternative to in vivo testing of oral care products, and provides insight into the behavior of the oral mucosal cells in healthy and pathological tissues. This review covers the...
Construction of a collagen-based, split-thickness cornea substitute
Acun, A.; Hasırcı, Vasıf Nejat (2014-08-01)
Tissue-engineered corneas may become a promising alternative to allografts in the treatment of serious cornea defects because of the tunable characteristics of the biomaterials, biomimetic designs, and incorporation of patient's own cells. In this study, collagen foam was coated with a fibrous mat to mimic the stromal layer and the Bowman's layer. The stromal layer substitute was made of N-ethyl-N-(3-dimethyl aminopropyl)carbodiimide/N-hydroxysuccinimide-cross-linked collagen-chondroitin sulfate foam and se...
Citation Formats
B. Kinikoglu, C. Auxenfans, P. Pierrillas, C. Burillon, V. N. Hasırcı, and O. Damour, “RECONSTRUCTION OF A FULL-THICKNESS COLLAGEN-BASED HUMAN ORAL MUCOSAL EQUIVALENT,” INTERNATIONAL JOURNAL OF ARTIFICIAL ORGANS, pp. 452–453, 2009, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/55839.