Ultra-rapid absorption of recombinant human insulin induced by zinc chelation and surface charge masking

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Date

2012-01-01

Author

POHL, Roderike
HAUSER, Robert
Lİ, Ming
De Souza, Errol
FELDSTEİN, Robert
SEİBERT, Richard
Özhan, Koray
Kashyap, Nandini
STEİNER, Solomon

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In order to enhance the absorption of insulin following subcutaneous injection, excipients were selected to hasten the dissociation rate of insulin hexamers and reduce their tendency to reassociate postinjection. A novel formulation of recombinant human insulin containing citrate and disodium ethylenediaminetetraacetic acid (EDTA) has been tested in clinic and has a very rapid onset of action in patients with diabetes. In order to understand the basis for the rapid insulin absorption, in vitro experiments using analytical ultracentrifugation, protein charge assessment, and light scattering have been performed with this novel human insulin formulation and compared with a commercially available insulin formulation [regular human insulin (RHI)].

Subject Keywords

Ultra-rapid absorption, Recombinant human insulin, Dynamic light scattering, Analytical ultracentrifugation

URI

https://hdl.handle.net/11511/30946

Journal

Journal of Diabetes Science and Technology

DOI

https://doi.org/10.1177/193229681200600404

Collections

Graduate School of Marine Sciences, Article

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Citation Formats

R. POHL et al., “Ultra-rapid absorption of recombinant human insulin induced by zinc chelation and surface charge masking,” Journal of Diabetes Science and Technology, pp. 755–763, 2012, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/30946.