Development of Silicalite/Glucose Oxidase-Based Biosensor and Its Application for Glucose Determination in Juices and Nectars

Download

10.1186:s11671-016-1275-2.pdf

Date

2016-02-03

Author

Dudchenko, Oleksandr Ye
Pyeshkova, Viktoriya M.
Soldatkin, Oleksandr O.
Akata Kurç, Burcu
Kasap, Berna O.
Soldatkin, Alexey P.
Dzyadevych, Sergei V.

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

174
views

93
downloads

The application of silicalite for improvement of enzyme adsorption on new stainless steel electrodes is reported. Glucose oxidase (GOx) was immobilized by two methods: cross-linking by glutaraldehyde (GOx-GA) and cross-linking by glutaraldehyde along with GOx adsorption on silicalite-modified electrode (SME) (GOx-SME-GA). The GOx-SME-GA biosensors were characterized by a four-to fivefold higher sensitivity than GOx-GA biosensor. It was concluded that silicalite together with GA sufficiently enhances enzyme adhesion on stainless steel electrodes. The developed GOx-SME-GA biosensors were characterized by good reproducibility of biosensor preparation (relative standard deviation (RSD)-18 %), improved signal reproducibility (RSD of glucose determination was 7 %), and good storage stability (29 % loss of activity after 18-day storage). A series of fruit juices and nectars was analyzed using GOx-SME-GA biosensor for determination of glucose concentration. The obtained results showed good correlation with the data of high-performance liquid chromatography (HPLC) (R = 0.99).

Subject Keywords

Enzyme immobilization, Silicalite, Glucose oxidase, Conductometric transducer, Biosensor

URI

https://hdl.handle.net/11511/31004

Journal

NANOSCALE RESEARCH LETTERS

DOI

https://doi.org/10.1186/s11671-016-1275-2

Collections

Graduate School of Natural and Applied Sciences, Article

Suggestions

OpenMETU
Core

Application of silicalite-modified electrode for the development of sucrose biosensor with improved characteristics Pyeshkova, Viktoriya M.; Dudchenko, Oleksandr Y.; Soldatkin, Oleksandr O.; Kasap, Berna Ozansoy; Lagarde, Florence; Akata Kurç, Burcu; Dzyadevych, Sergei V. (2015-03-27) The application of silicalite for improvement of working characteristics of conductometric enzyme biosensors for determination of sucrose was studied in this research. Biosensors based on different types of silicalite-modified electrodes were studied and compared according to their analytical characteristics. Polyethylenimine/glutaraldehyde/silicalite-modified biosensors showed higher sensitivity compared with others type of biosensors. Moreover, the polyethylenimine/glutaraldehyde/silicalite sucrose biosen...
Fabrication of a promising immobilization platform based on electrochemical synthesis of a conjugated polymer Buber, Ece; SÖYLEMEZ, SANİYE; UDUM, YASEMİN; Toppare, Levent Kamil (2018-07-01) Since conjugated polymers are an important class of materials with remarkable properties in biosensor applications, in this study, a novel glucose biosensor based on a conjugated polymer was fabricated via the electropolymerization of the monomer 10,13-bis(4-hexylthiophen-2-yl)dipyridol[3,2-a:2',3'-c]phenazine onto a graphite electrode surface. Glucose oxidase (GOx) was used as the model biological recognition element. As a result of the enzymatic reaction between GOx and glucose, the glucose amount was det...
Application of enzyme/zeolite sensor for urea analysis in serum Soldatkin, O. O.; Kucherenko, I. S.; Marchenko, S. V.; Kasap, B. Ozansoy; Akata Kurç, Burcu; Soldatkin, A. P.; Dzyadevych, S. V. (2014-09-01) Urea biosensor based on zeolite-adsorbed urease was applied for analysis of blood serum samples. It should be noted, that this biosensor has a number of advantages, such as simple and fast performance, the absence of toxic compounds during biosensor preparation, high reproducibility and repeatability (RSD = 9% and 4%, respectively). The linear range of urea determination by using the biosensor was 0.003-0.75 mM, and the limit of urea detection was 3 mu M. The method of standard addition was used for analysi...
A novel approach for the fabrication of a flexible glucose biosensor: The combination of vertically aligned CNTs and a conjugated polymer GOKOGLAN, Tugba Ceren; SOYLEMEZ, Saniye; KESİK, Melis; DOGRU, Itir Bakis; TUREL, Onur; YÜKSEL, Recep; Ünalan, Hüsnü Emrah; Toppare, Levent Kamil (2017-04-01) A novel flexible glucose biosensor using vertically aligned carbon nanotubes (VACNT) and a conjugated polymer (CP) was fabricated. A scaffold based on VACNT grown on aluminum foil (VACNT-Al foil) with poly (9,9-di-(2-ethylhexyl)-fluorenyl-2,7-diyl)-end capped with 2,5-diphenyl-1,2,4-oxadiazole (PFLO) was used as the immobilization matrix for the glucose biosensor. Glucose oxidase (GOx) was immobilized on a modified indium tin oxide (ITO) coated polyethylene terephthalate (PET) electrode surface. The biosens...
A Novel Amperometric Glutamate Biosensor Based on Glutamate Oxidase Adsorbed on Silicalite Soldatkina, O. V.; Soldatkin, O. O.; Kasap, B. Ozansoy; Kucherenko, D. Yu.; Kucherenko, I. S.; Akata Kurç, Burcu; Dzyadevych, S. V. (2017-04-07) In this work, we developed a new amperometric biosensor for glutamate detection using a typical method of glutamate oxidase (GlOx) immobilization via adsorption on silicalite particles. The disc platinum electrode (d = 0.4 mm) was used as the amperometric sensor. The procedure of biosensor preparation was optimized. The main parameters of modifying amperometric transducers with a silicalite layer were determined along with the procedure of GlOx adsorption on this layer. The biosensors based on GlOx adsorbed...

Citation Formats

O. Y. Dudchenko et al., “Development of Silicalite/Glucose Oxidase-Based Biosensor and Its Application for Glucose Determination in Juices and Nectars,” NANOSCALE RESEARCH LETTERS, pp. 0–0, 2016, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/31004.