Development of Silicalite/Glucose Oxidase-Based Biosensor and Its Application for Glucose Determination in Juices and Nectars

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10.1186:s11671-016-1275-2.pdf

Date

2016-02-03

Author

Dudchenko, Oleksandr Ye
Pyeshkova, Viktoriya M.
Soldatkin, Oleksandr O.
Akata Kurç, Burcu
Kasap, Berna O.
Soldatkin, Alexey P.
Dzyadevych, Sergei V.

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The application of silicalite for improvement of enzyme adsorption on new stainless steel electrodes is reported. Glucose oxidase (GOx) was immobilized by two methods: cross-linking by glutaraldehyde (GOx-GA) and cross-linking by glutaraldehyde along with GOx adsorption on silicalite-modified electrode (SME) (GOx-SME-GA). The GOx-SME-GA biosensors were characterized by a four-to fivefold higher sensitivity than GOx-GA biosensor. It was concluded that silicalite together with GA sufficiently enhances enzyme adhesion on stainless steel electrodes. The developed GOx-SME-GA biosensors were characterized by good reproducibility of biosensor preparation (relative standard deviation (RSD)-18 %), improved signal reproducibility (RSD of glucose determination was 7 %), and good storage stability (29 % loss of activity after 18-day storage). A series of fruit juices and nectars was analyzed using GOx-SME-GA biosensor for determination of glucose concentration. The obtained results showed good correlation with the data of high-performance liquid chromatography (HPLC) (R = 0.99).

Subject Keywords

Enzyme immobilization, Silicalite, Glucose oxidase, Conductometric transducer, Biosensor

URI

https://hdl.handle.net/11511/31004

Journal

NANOSCALE RESEARCH LETTERS

DOI

https://doi.org/10.1186/s11671-016-1275-2

Collections

Graduate School of Natural and Applied Sciences, Article

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Citation Formats

O. Y. Dudchenko et al., “Development of Silicalite/Glucose Oxidase-Based Biosensor and Its Application for Glucose Determination in Juices and Nectars,” NANOSCALE RESEARCH LETTERS, pp. 0–0, 2016, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/31004.