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Application of enzyme/zeolite sensor for urea analysis in serum
Date
2014-09-01
Author
Soldatkin, O. O.
Kucherenko, I. S.
Marchenko, S. V.
Kasap, B. Ozansoy
Akata Kurç, Burcu
Soldatkin, A. P.
Dzyadevych, S. V.
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Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License
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Urea biosensor based on zeolite-adsorbed urease was applied for analysis of blood serum samples. It should be noted, that this biosensor has a number of advantages, such as simple and fast performance, the absence of toxic compounds during biosensor preparation, high reproducibility and repeatability (RSD = 9% and 4%, respectively). The linear range of urea determination by using the biosensor was 0.003-0.75 mM, and the limit of urea detection was 3 mu M. The method of standard addition was used for analysis of serum samples with 500-fold dilution. Total time of analysis was 10 min. Good reproducibility of urea determination in real samples was demonstrated (RSD = 10%). Biosensor results were verified by using a common method of urea determination (diacetyl monoxime reaction). It was shown that by using this biosensor distinguishing healthy people from people with renal dysfunction becomes easier.
Subject Keywords
Silicalite
,
Enzyme adsorption
,
Urease
,
Conductometric transducer
,
Biosensor
URI
https://hdl.handle.net/11511/32027
Journal
MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
DOI
https://doi.org/10.1016/j.msec.2014.05.028
Collections
Graduate School of Natural and Applied Sciences, Article
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O. O. Soldatkin et al., “Application of enzyme/zeolite sensor for urea analysis in serum,”
MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
, pp. 155–160, 2014, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/32027.