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Polyinosinic: polycytidylic acid loading onto different generations of PAMAM dendrimer-coated magnetic nanoparticles
Date
2013-08-01
Author
Khodadust, Rouhollah
Mutlu, Pelin
Yalcin, Serap
Unsoy, Gozde
Gündüz, Ufuk
Metadata
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Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License
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Poly (I:C), which is a synthetic double-stranded RNA, have significant toxicity on tumor cells. The immobilization of Poly (I:C) onto nanoparticles is important for the fabrication of targeted delivery systems. In this study, different generations of newly synthesized PAMAM dendron-coated magnetic nanoparticles (DcMNP) which can be targeted to the tumor site under magnetic field were efficiently loaded for the first time with Poly (I:C). Different generations of DcMNPs (G(2), G(3), G(4), G(5), G(6), and G(7)) were synthesized. Poly (I:C) activation was achieved in the presence of EDC and 1-methylimidazole. Loading of Poly (I:C) onto DcMNPs was followed by agarose gel electrophoresis. Acidic reaction conditions were found as superior to basic and neutral for binding of Poly (I:C). In addition, having more functional groups at the surface, higher generations (G(7), G(6), and G(5)) of PAMAM DcMNPs were found more suitable as a delivery system for Poly (I:C). Further in vitro and in vivo analyses of Poly (I:C)/PAMAM magnetic nanoparticles may provide new opportunities for the selective targeting and killing of tumor cells.
Subject Keywords
Poly (I:C)
,
Magnetic nanoparticles
,
Targeted delivery system
,
Cancer therapy
URI
https://hdl.handle.net/11511/31052
Journal
JOURNAL OF NANOPARTICLE RESEARCH
DOI
https://doi.org/10.1007/s11051-013-1860-6
Collections
Graduate School of Natural and Applied Sciences, Article
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R. Khodadust, P. Mutlu, S. Yalcin, G. Unsoy, and U. Gündüz, “Polyinosinic: polycytidylic acid loading onto different generations of PAMAM dendrimer-coated magnetic nanoparticles,”
JOURNAL OF NANOPARTICLE RESEARCH
, pp. 0–0, 2013, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/31052.