Polyinosinic: polycytidylic acid loading onto different generations of PAMAM dendrimer-coated magnetic nanoparticles

Date

2013-08-01

Author

Khodadust, Rouhollah
Mutlu, Pelin
Yalcin, Serap
Unsoy, Gozde
Gündüz, Ufuk

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Poly (I:C), which is a synthetic double-stranded RNA, have significant toxicity on tumor cells. The immobilization of Poly (I:C) onto nanoparticles is important for the fabrication of targeted delivery systems. In this study, different generations of newly synthesized PAMAM dendron-coated magnetic nanoparticles (DcMNP) which can be targeted to the tumor site under magnetic field were efficiently loaded for the first time with Poly (I:C). Different generations of DcMNPs (G(2), G(3), G(4), G(5), G(6), and G(7)) were synthesized. Poly (I:C) activation was achieved in the presence of EDC and 1-methylimidazole. Loading of Poly (I:C) onto DcMNPs was followed by agarose gel electrophoresis. Acidic reaction conditions were found as superior to basic and neutral for binding of Poly (I:C). In addition, having more functional groups at the surface, higher generations (G(7), G(6), and G(5)) of PAMAM DcMNPs were found more suitable as a delivery system for Poly (I:C). Further in vitro and in vivo analyses of Poly (I:C)/PAMAM magnetic nanoparticles may provide new opportunities for the selective targeting and killing of tumor cells.

Subject Keywords

Poly (I:C), Magnetic nanoparticles, Targeted delivery system, Cancer therapy

URI

https://hdl.handle.net/11511/31052

Journal

JOURNAL OF NANOPARTICLE RESEARCH

DOI

https://doi.org/10.1007/s11051-013-1860-6

Collections

Graduate School of Natural and Applied Sciences, Article

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Citation Formats

R. Khodadust, P. Mutlu, S. Yalcin, G. Unsoy, and U. Gündüz, “Polyinosinic: polycytidylic acid loading onto different generations of PAMAM dendrimer-coated magnetic nanoparticles,” JOURNAL OF NANOPARTICLE RESEARCH, pp. 0–0, 2013, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/31052.