Cloning and expression of a plasmid-linked pediocin determinant trait of Pediococcus acidilactici F

2000-01-01
Osmanagaoglu, O
Beyatli, Y
Gündüz, Ufuk
Show full item record
Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
143
views
0
downloads
Plasmid DNA from Pediococcus acidilactici F was prepared by lysozyme-mutanolysin method and purified by cesium chloride-ethidium bromide (CsCl-EtBr) density gradient ultracentrifugation. Agarose gel electrophoresis of plasmid DNA and plasmid-curing experiments suggested that bacteriocin activity was harboured on a small plasmid of approximately 9.1 kb (kilobasepair) in Pediococcus acidilactici F. Plasmid encoding bacteriocin production in P. acidilactici F was examined for restriction enzyme cleavage patterns and its map has been constructed. An Escherichia coli strain transformed with the recombinant plasmid, pQE322, produced and, most probably, secreted pediocin F.
Bacteriocin, Ach, Pentosaceus, Inhibition, Nisin
https://hdl.handle.net/11511/31545
Graduate School of Natural and Applied Sciences, Article

Suggestions

Cloning and expression of the Clostridium thermocellum L-lactate dehydrogenase gene in Escherichia coli and enzyme characterization
Ozkan, M; Yilmaz, EI; Lynd, LR; Özcengiz, Gülay (Canadian Science Publishing, 2004-10-01)
The structural gene for L-lactate dehydrogenase (LDH) (EC.1.1.1.27) from Clostridium thermocellum 27405 was cloned in Escherichia coli by screening the Lambda Zap 11 phage library of C. thermocellum genomic DNA. In one positive clone, an open reading frame of 948 base pairs corresponded to C. thermocellum ldh gene encoding for the predicted 315-residue protein. The ldh gene was successfully expressed in E. coli FMJ39 (ldh mutant) under the lac promoter. The recombinant enzyme was partially purified from E. ...
Synthesis of ferrocenyl quinones and ferrocenyl based burning rate catalysts
Açıkalın, Serdar; Zora, Metin; Department of Chemistry (2003)
Recently, considerable interest has been devoted to the synthesis of new ferrocene derivatives since properly functionalized ferrocene derivatives could be potential antitumor substances. For this purpose, we have investigated the synthesis of ferrocenyl quinones starting from squaric acid. Thermolysis of ferrocenylsubstituted cyclobutenones, which have been prepared from ferrocenyl cyclobutenediones and alkenyllithiums, affords hydroquinones, which furnish, upon oxidation, ferrocenyl quinones. Ferrocenyl c...
Ceria-supported ruthenium nanoparticles as highly active and long-lived catalysts in hydrogen generation from the hydrolysis of ammonia borane
Akbayrak, Serdar; Tonbul, Yalcin; Özkar, Saim (2016-01-01)
Ruthenium(0) nanoparticles supported on ceria (Ru-0/CeO2) were in situ generated from the reduction of ruthenium(III) ions impregnated on ceria during the hydrolysis of ammonia borane. Ru-0/CeO2 was isolated from the reaction solution by centrifugation and characterized by ICP-OES, BET, XRD, TEM, SEM-EDS and XPS techniques. All the results reveal that ruthenium(0) nanoparticles were successfully supported on ceria and the resulting Ru-0/CeO2 is a highly active, reusable and long-lived catalyst for hydrogen ...
Enantioselective direct aldol reactions promoted by phosphine oxide aziridinyl phosphonate organocatalysts
Doğan, Özdemir (2015-12-15)
A series of phosphine oxide based chiral Lewis bases were screened as organocatalysts for silicon tetrachloride mediated direct asymmetric aldol reactions between cyclohexanone and various aromatic aldehydes. One of the phosphine oxide-aziridinyl phosphonate POAP-A Lewis bases formed the aldol products in up to 75% yield and with 65% ee.
Cloning and heterologous expression of chlorophyll a synthase in Rhodobacter sphaeroides
Ipekoglu, Emre M.; Gocmen, Koray; Oz, Mehmet T.; Gurgan, Muazzez; Yucel, Meral (2017-03-01)
Rhodobacter sphaeroides is a purple non-sulfur bacterium which photoheterotrophically produces hydrogen from organic acids under anaerobic conditions. A gene coding for putative chlorophyll a synthase (chlG) from cyanobacterium Prochlorococcus marinus was amplified by nested polymerase chain reaction and cloned into an inducible-expression plasmid which was subsequently transferred to R. sphaeroides for heterologous expression. Induced expression of chlG in R. sphaeroides led to changes in light absorption ...
Citation Formats
O. Osmanagaoglu, Y. Beyatli, and U. Gündüz, “Cloning and expression of a plasmid-linked pediocin determinant trait of Pediococcus acidilactici F,” JOURNAL OF BASIC MICROBIOLOGY, pp. 41–49, 2000, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/31545.
METU IIS - Integrated Information Service open@metu.edu.tr