Osteogenic differentiation of adipose derived stem cells on high and low aspect ratio micropatterns

Date

2015-12-12

Author

Sayin, Esen
Baran, Erkan Turker
Hasırcı, Vasıf Nejat

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Adipose derived stem cells (ADSCs) were cultured on collagen-silk fibroin films with microchannel and micropillar patterns to investigate the effects of cell morphology changes on osteogenic differentiation. Channel and pillar micropatterned films were prepared from collagen type I and silk fibroin. While higher ADSC proliferation profiles were obtained on micropillar blend film, microchannel blend films, however, caused twice higher aspect ratio and effective orientation of cells. Alkaline phosphatase activity of ADSCs was several times higher on microchannel surface when the measured activities were normalized to cell number. Effective deposition of collagen type I and mineral by the cells were observed for patterned and unpatterned films, and these extracellular matrix components were oriented along the axis of the microchannels. In conclusion, the use of collagen-fibroin blend film with microchannel topography increased the aspect ratio and alignment of cells significantly, and was also effective in the differentiation of ADSCs into osteogenic lineage

Subject Keywords

Osteogenesis, Silk fibroin, Collagen, Micropattern, Adipose derived stem cells

URI

https://hdl.handle.net/11511/31950

Journal

JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION

DOI

https://doi.org/10.1080/09205063.2015.1100494

Collections

Graduate School of Natural and Applied Sciences, Article

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Citation Formats

E. Sayin, E. T. Baran, and V. N. Hasırcı, “Osteogenic differentiation of adipose derived stem cells on high and low aspect ratio micropatterns,” JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION, pp. 1402–1424, 2015, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/31950.