Osteogenic differentiation of MC3T3-E1 cells on different titanium surfaces

Bozkurt, S. Buket
Koc, Nursen
Timucin, Muharrem
Korkusuz, Feza
mRNA expressions related to osteogenic differentiation of MC3T3-E1 cells on electro-polished smooth (S), sandblasted small-grit (SSG) and sandblasted large-grit (SLG) surfaces of titanium alloys were investigated in vitro. Gene expression profiles of cells were evaluated using the RT2 Profiler PCR microarray on day 7. Mineralizing tissue-associated proteins, differentiation factors and extracellular matrix enzymes mRNA expressions were measured using Q-PCR. SLG surface upregulated 23 genes over twofolds and downregulated 3 genes when compared to the S surface. In comparison to the SSG surface, at least a twofold increase in 25 genes was observed in the SLG surface. BSP, OCN, OPN, COL I and ALP mRNA expressions increased in the SLG group when compared to the S and the SSG groups. BMP-2, BMP-6 and TGF-beta mRNA expressions increased in both the SSG and the SLG surfaces. MMP-2 and MMP-9 mRNA expressions increased as the surface roughness increased. This study demonstrated that surface roughness of titanium implants has a significant effect on cellular behavior and SLG surface apparently increased gene expressions related to osteogenesis when compared to the S and the SSG surfaces.


Cellulose acetate-gelatin-coated boron-bioactive glass biocomposite scaffolds for bone tissue engineering
Rad, Reza Moonesi; ALSHEMARY, AMMAR ZEIDAN GHAILAN; Evis, Zafer; Keskin, Dilek; Tezcaner, Ayşen (IOP Publishing, 2020-11-01)
In this study, we aimed to prepare and characterize porous scaffolds composed of pure and boron oxide (B2O3)-doped bioactive glass (BG) that were infiltrated by cellulose acetate-gelatin (CA-GE) polymer solution for bone tissue engineering applications. Composite scaffolds were cross-linked with glutaraldehyde after polymer coating to protect the structural integrity of the polymeric-coated scaffolds. The impact of B(2)O(3)incorporation into BG-polymer porous scaffolds on the cross-sectional morphology, por...
Covalent immobilization of chloroperoxidase onto magnetic beads: Catalytic properties and stability
Bayramoglu, Guelay; Kiralp, Senem; Yilmaz, Meltem; Toppare, Levent Kamil; Arica, M. Yakup (Elsevier BV, 2008-02-15)
Amino groups containing magnetic beads were used in covalent immobilization of the enzyme "chloroperoxidase (CPO)" which is one of a few enzymes that can catalyse the peroxide dependent oxidation of a wide spectrum of organic and inorganic compounds. The magnetic poly(glycidylmethacrylate-methylmethacrylate-etbyleneglycol dimethacrylate), magnetic p(GMA-MMA-EGDMA) beads were prepared via suspension polymerization in the presence of ferric ions. The magnetic beads were characterized with scanning electron mi...
Immobilization of glucose oxidase onto gelatin for biosensor construction
Emregul, E; Sungur, S; Akbulut, Ural (Informa UK Limited, 2005-01-01)
The properties of a glucose biosensor made by immobilization of glucose oxidase onto gelatin in a layer of electrochemically deposited polyaniline have been investigated. Glucose oxidase was immobilized within gelatin cross-links with chromium(III) acetate. The glucose oxidase biosensor was developed by forming a polyaniline-deposited electrode surface as support for the immobilized enzyme gel, in order to increase its durability. The polyaniline/gelatin/glucose oxidase biosensor has been characterized usin...
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Onat, Bora; Tuncer, Sinem; Ulusan, Sinem; Banerjee, Sreeparna; Erel Göktepe, İrem (IOP Publishing, 2019-07-01)
Biodegradable polymers have been broadly used as agents that can complex with and deliver osteoinductive agents, but osteoinductivity of the polymers themselves has been rarely studied. Here we report the osteoinductivity of poly(4-hydroxy-L-proline ester) (PHPE), a biodegradable cationic polymer with cell penetrating properties. Under physiological conditions, PHPE degrades into trans-4-hydroxy-L-prohne (trans-Hyp), a non-coded amino acid with essential functions in collagen fibril formation and fibril sta...
Biodegradation of 4-CP and 2,4-DCP mixture in a rotating biological contactor (RBC)
şahinkaya, Erkan; Dilek, Filiz Bengü (Elsevier BV, 2006-09-01)
In this study, the performance of a two stage rotating biological contactor (RBC) was evaluated for the treatment of synthetic wastewater containing peptone, 4-chlorophenol (4-CP) and 2,4-dichlorophenol (2,4-DCP) at 5 rpm. Also, the effect of biogenic substrate (peptone) concentration on the reactor performance was investigated. High chlorophenols (> 98%) and COD (> 94%) removals were achieved throughout the reactor operation in the first stage and the second stage behaved as a polishing step. The observed ...
Citation Formats
S. HAKKI et al., “Osteogenic differentiation of MC3T3-E1 cells on different titanium surfaces,” BIOMEDICAL MATERIALS, pp. 0–0, 2012, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/57778.