Osteogenic differentiation of MC3T3-E1 cells on different titanium surfaces

Date

2012-08-01

Author

HAKKI, SEMA
Bozkurt, S. Buket
HAKKI, ERDOĞAN EŞREF
KORKUSUZ, PETEK
PURALI, NUHAN
Koc, Nursen
Timucin, Muharrem
ÖZTÜRK, ADNAN
Korkusuz, Feza

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mRNA expressions related to osteogenic differentiation of MC3T3-E1 cells on electro-polished smooth (S), sandblasted small-grit (SSG) and sandblasted large-grit (SLG) surfaces of titanium alloys were investigated in vitro. Gene expression profiles of cells were evaluated using the RT2 Profiler PCR microarray on day 7. Mineralizing tissue-associated proteins, differentiation factors and extracellular matrix enzymes mRNA expressions were measured using Q-PCR. SLG surface upregulated 23 genes over twofolds and downregulated 3 genes when compared to the S surface. In comparison to the SSG surface, at least a twofold increase in 25 genes was observed in the SLG surface. BSP, OCN, OPN, COL I and ALP mRNA expressions increased in the SLG group when compared to the S and the SSG groups. BMP-2, BMP-6 and TGF-beta mRNA expressions increased in both the SSG and the SLG surfaces. MMP-2 and MMP-9 mRNA expressions increased as the surface roughness increased. This study demonstrated that surface roughness of titanium implants has a significant effect on cellular behavior and SLG surface apparently increased gene expressions related to osteogenesis when compared to the S and the SSG surfaces.

Subject Keywords

Bioengineering, Biomaterials, Biomedical Engineering

URI

https://hdl.handle.net/11511/57778

Collections

Department of Physical Education and Sports, Article