A novel architecture based on a conducting polymer and calixarene derivative: its synthesis and biosensor construction

Date

2015-01-01

Author

GOKOGLAN, Tugba Ceren
SOYLEMEZ, Saniye
KESIK, Melis
UNAY, Hande
Sayin, Serkan
Yildiz, Huseyin Bekir
Çırpan, Ali
Toppare, Levent Kamil

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In this study, a novel amperometric glucose biosensor based on a selenium comprising conducting polymer and calixarene was developed. Firstly, poly(2-(2-octyldodecyl)-4,7-di(selenoph-2-yl)-2H-benzo[d][1,2,3]-triazole), poly((SBTz)) was electrodeposited onto a graphite electrode by an electropolymerization technique. Then, a newly synthesized calixarene and gold nanoparticle (AuNP) mixture was used for the improvement of biosensor characteristics. GOx, as a model enzyme was immobilized on the modified electrode surface. The constructed surface serves as a sufficient immobilization platform for the detection of glucose. Calixarenes and their derivatives may be a favouring agent for enzyme immobilization due to their specific configurations. Moreover, through the covalent binding between the carboxylic groups of the calixarenes and amino groups of the biomolecule, effective enzyme immobilization can be achieved while protecting the well-ordered structure of the enzyme molecule. Amperometric detection was carried out following oxygen consumption at -0.7 V vs. the Ag reference electrode in phosphate buffer (50 mM, pH 6.5). The proposed biosensor showed a linear amperometric response for glucose within a concentration range of 0.005 to 0.5 mM (LOD: 0.004 mM). K-m(app) and sensitivity were calculated as 0.025 mM and 102 mu A mM(-1) cm(-2), respectively. Scanning Electron Microscopy (SEM) was used to investigate the surface morphologies of successive modifications. Finally, the constructed biosensor was tested successfully to detect glucose in beverage samples.

Subject Keywords

Glucose-oxidase, Immobilization matrix, Enzyme immobilization, Gold nanoparticle, Biomolecule immobilization, Electrochemical biosensors, Amperometric biosensors, Redox, Films, Entrapment

URI

https://hdl.handle.net/11511/41570

Journal

RSC ADVANCES

DOI

https://doi.org/10.1039/c5ra03933a

Collections

Department of Chemistry, Article

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A conducting polymer and a calixarene derivative A novel surface design for glucose detection Gökoğlan, Ceren; Söylemez, Saniye; Kesik, Melis; Ünay, Hande; Sayın, Serkan; Çırpan, Ali; Yıldız, Hüseyin Bekir; Toppare, Levent Kamil (null; 2016-07-17) In this study, a novel amperometric glucose biosensor based on a conducting polymer and a calixarene was developed. Conducting polymer of (2‐(2‐oc‐tyldodecyl)‐4,7‐di(selenoph‐2‐yl)‐2H‐benzo[d][1,2,3]triazole)) (SBTz) was used as the immobilization matrix for biomolecule deposition to achieve an effective surface design to detect glucose. After successful deposition of SBTz on graphite electrode, a newly synthesized calixarene and gold nanoparticle (AuNP) mixture were used for improving biosensor character...
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An effective surface design based on a conjugated polymer and silver nanowires for the detection of paraoxon in tap water and milk Turan, Janset; Kesik, Melis; SÖYLEMEZ, SANİYE; Goker, Seza; Coskun, Sahin; Ünalan, Hüsnü Emrah; Toppare, Levent Kamil (2016-06-02) In this study, a novel approach for the fabrication of a biosensor utilizing a conducting polymer and silver nanowires is reported. To obtain immobilization platform for butyrylcholinesterase (BChE), a graphite electrode was modified with the poly(5,6-bis(octyloxy)-4,7-di(thieno[3][3,2-b]thiophen-2yl)benzo[c][1,2,5]oxoadiazole) (PTTBO) which has a hydrophobic alkyl chain as the pendant group providing hydrophobic nature to the matrix. Since biomolecules contain both hydrophobic and hydrophilic parts in thei...
Selenium containing conducting polymer based pyranose oxidase biosensor for glucose detection Gokoglan, Tugba Ceren; SÖYLEMEZ, SANİYE; Kesik, Melis; Toksabay, Sinem; Toppare, Levent Kamil (2015-04-01) A novel amperometric pyranose oxidase (PyOx) biosensor based on a selenium containing conducting polymer has been developed for the glucose detection. For this purpose, a conducting polymer; poly(4,7-bis(thieno[3,2-b]thiophen-2-yl)benzo[c][1,2,5] selenadiazole) (poly(BSeTT)) was synthesized via electropolymerisation on gold electrode to examine its matrix property for glucose detection. For this purpose, PyOx was used as the model enzyme and immobilised via physical adsorption technique. Amperometric detect...

Citation Formats

T. C. GOKOGLAN et al., “A novel architecture based on a conducting polymer and calixarene derivative: its synthesis and biosensor construction,” RSC ADVANCES, pp. 35940–35947, 2015, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/41570.