A novel architecture based on a conducting polymer and calixarene derivative: its synthesis and biosensor construction

GOKOGLAN, Tugba Ceren
KESIK, Melis
UNAY, Hande
Sayin, Serkan
Yildiz, Huseyin Bekir
Çırpan, Ali
Toppare, Levent Kamil
In this study, a novel amperometric glucose biosensor based on a selenium comprising conducting polymer and calixarene was developed. Firstly, poly(2-(2-octyldodecyl)-4,7-di(selenoph-2-yl)-2H-benzo[d][1,2,3]-triazole), poly((SBTz)) was electrodeposited onto a graphite electrode by an electropolymerization technique. Then, a newly synthesized calixarene and gold nanoparticle (AuNP) mixture was used for the improvement of biosensor characteristics. GOx, as a model enzyme was immobilized on the modified electrode surface. The constructed surface serves as a sufficient immobilization platform for the detection of glucose. Calixarenes and their derivatives may be a favouring agent for enzyme immobilization due to their specific configurations. Moreover, through the covalent binding between the carboxylic groups of the calixarenes and amino groups of the biomolecule, effective enzyme immobilization can be achieved while protecting the well-ordered structure of the enzyme molecule. Amperometric detection was carried out following oxygen consumption at -0.7 V vs. the Ag reference electrode in phosphate buffer (50 mM, pH 6.5). The proposed biosensor showed a linear amperometric response for glucose within a concentration range of 0.005 to 0.5 mM (LOD: 0.004 mM). K-m(app) and sensitivity were calculated as 0.025 mM and 102 mu A mM(-1) cm(-2), respectively. Scanning Electron Microscopy (SEM) was used to investigate the surface morphologies of successive modifications. Finally, the constructed biosensor was tested successfully to detect glucose in beverage samples.


A conducting polymer and a calixarene derivative A novel surface design for glucose detection
Gökoğlan, Ceren; Söylemez, Saniye; Kesik, Melis; Ünay, Hande; Sayın, Serkan; Çırpan, Ali; Yıldız, Hüseyin Bekir; Toppare, Levent Kamil (null; 2016-07-17)
In this study, a novel amperometric glucose biosensor based on a conducting polymer and a calixarene was developed. Conducting polymer of (2‐(2‐oc‐tyldodecyl)‐4,7‐di(selenoph‐2‐yl)‐2H‐benzo[d][1,2,3]triazole)) (SBTz) was used as the immobilization matrix for biomolecule deposition to achieve an effective surface design to detect glucose. After successful deposition of SBTz on graphite electrode, a newly synthesized calixarene and gold nanoparticle (AuNP) mixture were used for improving biosensor character...
A Novel Amperometric Glutamate Biosensor Based on Glutamate Oxidase Adsorbed on Silicalite
Soldatkina, O. V.; Soldatkin, O. O.; Kasap, B. Ozansoy; Kucherenko, D. Yu.; Kucherenko, I. S.; Akata Kurç, Burcu; Dzyadevych, S. V. (2017-04-07)
In this work, we developed a new amperometric biosensor for glutamate detection using a typical method of glutamate oxidase (GlOx) immobilization via adsorption on silicalite particles. The disc platinum electrode (d = 0.4 mm) was used as the amperometric sensor. The procedure of biosensor preparation was optimized. The main parameters of modifying amperometric transducers with a silicalite layer were determined along with the procedure of GlOx adsorption on this layer. The biosensors based on GlOx adsorbed...
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In this study, immobilization of laccase (L) enzyme on magnetite (Fe(3)O(4)) nanoparticles was achieved, so that the immobilized enzyme could be used repeatedly. For this purpose, Fe(3)O(4) nanoparticles were coated and functionalized with chitosan (CS) and laccase from Trametes versicolor was immobilized onto chitosan-coated magnetic nanoparticles (Fe(3)O(4)-CS) by adsorption or covalent binding after activating the hydroxyl groups of chitosan with carbodiimide (EDAC) or cyanuric chloride (CC). For chitosa...
A novel approach for the fabrication of a flexible glucose biosensor: The combination of vertically aligned CNTs and a conjugated polymer
GOKOGLAN, Tugba Ceren; SOYLEMEZ, Saniye; KESİK, Melis; DOGRU, Itir Bakis; TUREL, Onur; YÜKSEL, Recep; Ünalan, Hüsnü Emrah; Toppare, Levent Kamil (2017-04-01)
A novel flexible glucose biosensor using vertically aligned carbon nanotubes (VACNT) and a conjugated polymer (CP) was fabricated. A scaffold based on VACNT grown on aluminum foil (VACNT-Al foil) with poly (9,9-di-(2-ethylhexyl)-fluorenyl-2,7-diyl)-end capped with 2,5-diphenyl-1,2,4-oxadiazole (PFLO) was used as the immobilization matrix for the glucose biosensor. Glucose oxidase (GOx) was immobilized on a modified indium tin oxide (ITO) coated polyethylene terephthalate (PET) electrode surface. The biosens...
A New Amperometric Biosensor for Diamine: Use of a Conducting Polymer Layer
Al Layla, Alaa M. T.; Turkarslan, Ozlem; KURBANOĞLU, SEVİNÇ; Sulaiman, Saddalah T.; Al-Flayeh, K. A.; Toppare, Levent Kamil (2013-01-01)
Diamine oxidase (DAO) from Porcine kidney E.C. is used for the amperometric detection of biogenic amines. Immobilization of enzyme is performed via entrapment in conducting poly (3,4-ethylenedioxythiophene) during electrochemical polymerization. The responses of the enzyme electrodes are measured via monitoring current at +0.7 V. In order to minimize the common interferences, an over oxidized film of polypyrrole is introduced to the electrode. Thickness of the film, kinetic parameters, substrate spe...
Citation Formats
T. C. GOKOGLAN et al., “A novel architecture based on a conducting polymer and calixarene derivative: its synthesis and biosensor construction,” RSC ADVANCES, pp. 35940–35947, 2015, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/41570.