Xanthan-gelatin and xanthan-gelatin-keratin wound dressings for local delivery of vitamin c

Demir, Gizem Çiğdem
In recent years, functional dressings that can protect the wound area from dehydration, support healing (interactive), and protect the wound against infections have gained importance instead of dry and passive dressings. In this study, it was aimed to develop temporary xanthan gelatin (XGH) and keratin xanthan gelatin hydrogels (KXGHs) that have high exudate absorption capacity, oxygen permeability, and applicability as a wound dressing for medical applications. Firstly, xanthan gelatin hydrogels were produced using different glycerol concentrations. Then, different keratin concentrations were added into xanthan/gelatin hydrogels to determine optimum keratin concentrations. The produced XGH and KXGHs were immersed into different Vitamin C (VC) solutions for loading. Physical properties, mechanical strength, biodegradability, FTIR, DSC, TGA analysis, and biocompatibility of XGH, KXGH, and VC-loaded KXGH (VC-KXGHs) were analyzed. The addition of keratin to XGH improved the viability of L929 fibroblast cells, increased the protein release from the hydrogels. The addition of VC and keratin didn’t affect the water vapor transmission rate and oxygen permeability vi significantly. However, VC immersed hydrogels showed a higher water uptake percentage and higher weight loss at the end of the 5th day. VC was released in 100h. VC loaded hydrogels increased collagen synthesis by the fibroblast cells. Also, microbial penetration test proved that XGH, and KXGH, can be a good barrier against bacteria. Therefore, VC-XGH and VC-KXGH can be suggested to hold potential as candidate wound dressing material for skin wounds.


EGF containing gelatin-based wound dressings
Ulubayram, K; Cakar, AN; Korkusuz, P; Ertan, C; Hasırcı, Nesrin (2001-06-01)
In case of bulk loss of tissue or non-healing wounds such as burns, trauma, diabetic, decubitus and venous stasis ulcers, a proper wound dressing is needed to cover the wound area, protect the damaged tissue, and if possible to activate the cell proliferation and stimulate the healing process. In this study, synthesis of a novel polymeric bilayer wound dressing containing epidermal growth-factor (EGF)-loaded microspheres was aimed. For this purpose, a natural, nontoxic and biocompatible material, gelatin, w...
Polymeric materials in wound healing
Ulubayram, K; Hasırcı, Nesrin (1997-09-17)
There is a high need for rapid healing in the treatment of severe burns, trauma, diabetic, decubitus ulcers and other conditions where a great damage of the tissue is exist. In those cases, wound should be covered with a dressing which replaces the functions of the natural skin by protecting the loss of body fluid and proteins, preventing bacterial invasion, improving and stimulating the healing process by providing a support for the proliferating cells
Addition of Selenium Nanoparticles to Electrospun Silk Scaffold Improves the Mammalian Cell Activity While Reducing Bacterial Growth
Chung, Stanley; Ercan, Batur; Roy, Amit K.; Webster, Thomas J. (2016-07-14)
Silk possesses many beneficial wound healing properties, and electrospun scaffolds are especially applicable for skin applications, due to their smaller interstices and higher surface areas. However, purified silk promotes microbial growth. Selenium nanoparticles have shown excellent antibacterial properties and are a novel antimicrobial chemistry. Here, electrospun silk scaffolds were doped with selenium nanoparticles to impart antibacterial properties to the silk scaffolds. Results showed significantly im...
Corrosion Resistance and Cytocompatibility of Magnesium-Calcium Alloys Modified with Zinc- or Gallium-Doped Calcium Phosphate Coatings
TAMAY, DİLARA GÖKSU; Gokyer, Seyda; Schmidt, Jurgen; Vladescu, Alina; HURİ, PINAR; Hasırcı, Vasıf Nejat; Hasırcı, Nesrin (2022-01-12)
In orthopedic surgery, metals are preferred to support or treat damaged bones due to their high mechanical strength. However, the necessity for a second surgery for implant removal after healing creates problems. Therefore, biodegradable metals, especially magnesium (Mg), gained importance, although their extreme susceptibility to galvanic corrosion limits their applications. The focus of this study was to control the corrosion of Mg and enhance its biocompatibility. For this purpose, surfaces of magnesium-...
Optimizing Mesenchymal Stem Cell Wound Healing Properties on Electrospun Silk with Selenium Nanoparticles
Chung, Stanley; Stolzoff, Mıchelle; Tran, Phong; Ercan, Batur; Webster, Thomas (2015-12-31)
Statement of Purpose: Current treatments for skin wound healing provide limited healing and do not promote skin repair. Therefore, an alternative approach that actively participates in the endogenous wound healing process would significantly improve wound healing and expand the scope of skin treatment to more serious conditions, such as severe burns and epidermal replacement. In addition to their stemness properties, mesenchymal stem cells (MSCs) have a number of cell signaling properties that are very bene...
Citation Formats
G. Ç. Demir, “Xanthan-gelatin and xanthan-gelatin-keratin wound dressings for local delivery of vitamin c,” M.S. - Master of Science, Middle East Technical University, 2021.