Xanthan-gelatin and xanthan-gelatin-keratin wound dressings for local delivery of vitamin c

Demir, Gizem Çiğdem
In recent years, functional dressings that can protect the wound area from dehydration, support healing (interactive), and protect the wound against infections have gained importance instead of dry and passive dressings. In this study, it was aimed to develop temporary xanthan gelatin (XGH) and keratin xanthan gelatin hydrogels (KXGHs) that have high exudate absorption capacity, oxygen permeability, and applicability as a wound dressing for medical applications. Firstly, xanthan gelatin hydrogels were produced using different glycerol concentrations. Then, different keratin concentrations were added into xanthan/gelatin hydrogels to determine optimum keratin concentrations. The produced XGH and KXGHs were immersed into different Vitamin C (VC) solutions for loading. Physical properties, mechanical strength, biodegradability, FTIR, DSC, TGA analysis, and biocompatibility of XGH, KXGH, and VC-loaded KXGH (VC-KXGHs) were analyzed. The addition of keratin to XGH improved the viability of L929 fibroblast cells, increased the protein release from the hydrogels. The addition of VC and keratin didn’t affect the water vapor transmission rate and oxygen permeability vi significantly. However, VC immersed hydrogels showed a higher water uptake percentage and higher weight loss at the end of the 5th day. VC was released in 100h. VC loaded hydrogels increased collagen synthesis by the fibroblast cells. Also, microbial penetration test proved that XGH, and KXGH, can be a good barrier against bacteria. Therefore, VC-XGH and VC-KXGH can be suggested to hold potential as candidate wound dressing material for skin wounds.


Xanthan-gelatin and xanthan-gelatin-keratin wound dressings for local delivery of Vitamin C
Demir, Gizem Cigdem; Erdemli, Özge; Keskin, Dilek; Tezcaner, Ayşen (2022-02-01)
Recently, functional dressings that can protect the wound area from dehydration and bacterial infection and support healing have gained importance in place of passive dressings. This study aimed to develop temporary and regenerative xanthan/gelatin (XGH) and keratin/xanthan/gelatin hydrogels (KXGHs) that have high absorption capacity and applicability as a wound dressing that can provide local delivery of Vitamin C (VC). Firstly, xanthan/gelatin hydrogels were produced by crosslinking with different glycero...
EGF containing gelatin-based wound dressings
Ulubayram, K; Cakar, AN; Korkusuz, P; Ertan, C; Hasırcı, Nesrin (2001-06-01)
In case of bulk loss of tissue or non-healing wounds such as burns, trauma, diabetic, decubitus and venous stasis ulcers, a proper wound dressing is needed to cover the wound area, protect the damaged tissue, and if possible to activate the cell proliferation and stimulate the healing process. In this study, synthesis of a novel polymeric bilayer wound dressing containing epidermal growth-factor (EGF)-loaded microspheres was aimed. For this purpose, a natural, nontoxic and biocompatible material, gelatin, w...
Nakipoğlu, Mustafa; Tezcaner, Ayşen; Özen, Can; Department of Biotechnology (2023-1-18)
Despite the advancement of modern technology, wound care is still based on traditional methods such as suture, staple and gauze around the world. Although, these methods provide affordable treatment for various types of wounds, they fall short in easy and effective treatment to facilitate healing process. Enabling a quicker and more convenient wound healing necessitates a comprehensive approach to wound dressing development to include simple yet multifunctional and versatile designs. In this work, we focuse...
Polymeric materials in wound healing
Ulubayram, K; Hasırcı, Nesrin (1997-09-17)
There is a high need for rapid healing in the treatment of severe burns, trauma, diabetic, decubitus ulcers and other conditions where a great damage of the tissue is exist. In those cases, wound should be covered with a dressing which replaces the functions of the natural skin by protecting the loss of body fluid and proteins, preventing bacterial invasion, improving and stimulating the healing process by providing a support for the proliferating cells
Addition of Selenium Nanoparticles to Electrospun Silk Scaffold Improves the Mammalian Cell Activity While Reducing Bacterial Growth
Chung, Stanley; Ercan, Batur; Roy, Amit K.; Webster, Thomas J. (2016-07-14)
Silk possesses many beneficial wound healing properties, and electrospun scaffolds are especially applicable for skin applications, due to their smaller interstices and higher surface areas. However, purified silk promotes microbial growth. Selenium nanoparticles have shown excellent antibacterial properties and are a novel antimicrobial chemistry. Here, electrospun silk scaffolds were doped with selenium nanoparticles to impart antibacterial properties to the silk scaffolds. Results showed significantly im...
Citation Formats
G. Ç. Demir, “Xanthan-gelatin and xanthan-gelatin-keratin wound dressings for local delivery of vitamin c,” M.S. - Master of Science, Middle East Technical University, 2021.