Xanthan-gelatin and xanthan-gelatin-keratin wound dressings for local delivery of vitamin c

Demir, Gizem Çiğdem
In recent years, functional dressings that can protect the wound area from dehydration, support healing (interactive), and protect the wound against infections have gained importance instead of dry and passive dressings. In this study, it was aimed to develop temporary xanthan gelatin (XGH) and keratin xanthan gelatin hydrogels (KXGHs) that have high exudate absorption capacity, oxygen permeability, and applicability as a wound dressing for medical applications. Firstly, xanthan gelatin hydrogels were produced using different glycerol concentrations. Then, different keratin concentrations were added into xanthan/gelatin hydrogels to determine optimum keratin concentrations. The produced XGH and KXGHs were immersed into different Vitamin C (VC) solutions for loading. Physical properties, mechanical strength, biodegradability, FTIR, DSC, TGA analysis, and biocompatibility of XGH, KXGH, and VC-loaded KXGH (VC-KXGHs) were analyzed. The addition of keratin to XGH improved the viability of L929 fibroblast cells, increased the protein release from the hydrogels. The addition of VC and keratin didn’t affect the water vapor transmission rate and oxygen permeability vi significantly. However, VC immersed hydrogels showed a higher water uptake percentage and higher weight loss at the end of the 5th day. VC was released in 100h. VC loaded hydrogels increased collagen synthesis by the fibroblast cells. Also, microbial penetration test proved that XGH, and KXGH, can be a good barrier against bacteria. Therefore, VC-XGH and VC-KXGH can be suggested to hold potential as candidate wound dressing material for skin wounds.
Citation Formats
G. Ç. Demir, “Xanthan-gelatin and xanthan-gelatin-keratin wound dressings for local delivery of vitamin c,” M.S. - Master of Science, Middle East Technical University, 2021.