Anticarcinogenic effects of the ethanolic extract from salix aegyptiaca l. in colon cancer, involvement of akt pathway

Download
2013
Enayat, Shabnam
Salix aegyptiaca has been used for centuries as a medicinal plant for various health disorders. Our previous study demonstrated high contents of phenolics and antioxidants in its bark many of those are famed for their anti-inflammatory properties. Since inflammation frequently accompanies the progress of colorectal cancer, in the current study we showed that the ethanolic extract of bark (EEB) from S.aegyptiaca exerted strong anti-proliferative effects on several cancer cells but not on non-cancerous CCD-18Co cells. Ethyl acetate and water fractions, obtained by solvent-solvent fractionation of EEB showed strong antiproliferative effects comparable to the effect from EEB. Both fractions were applied to tandom mass spectrometry. Catechin, salicin and catechol in Ethyl acetate and salicin in aqueous fractions were the most abundant compounds. Additionally, treatment with the extract induced apoptosis and cell cycle arrest at G1/S, not by DNA damage, but by decreasing phosphorylation and activity of Akt/PKB, stabilizing p53 and upregulating p21. The extract strongly reduced the superoxide anion production in cancer cells but not normal cells. In addition, EEB strongly inhibited anchorage independent growth of colon cancer cell lines HCT-116 and HT-29. Moreover, EEB could inhibit the motility, determined by scratch wound healing and migration through Transwells and adhesion of cancer cells to fibronectin. These in vitro functional changes were accompanied by the downregulation of the EMT markers SNAI1, SNAI2, Twist1, MMP9 and MMP2 and a restoration of E-cadherin expression. We propose that EEB from S.aegyptiaca, with its myriad cancer chemopreventive effects, can be consumed for its health promoting effects.

Suggestions

Antioxidant and cytotoxic properties of Salvia fruticosa M. and its effects on gene expressions of some CYP450 and antioxidant enzymes in HT-29 cell line
Altay, Ahmet; Bozoğlu, Faruk; Tokuşoğlu, Özlem; Department of Biochemistry (2015)
Salvia genus is a widely cultivated as a perennial herb and used in medicine for various purposes due to its antimicrobial, antioxidant, anticarcinogen and anti-inflammatory properties. In this study, the phenolic composition of water extract of Salvia fruticosa and possible effects of those constituents in cancer related drug metabolizing enzymes are investigated. S. fruticosa extract displayed high Radical Scavenging Activity against DPPH and ABTS radicals and also showed high Fe2+ Chelating Activity . To...
Anticarcinogenic Effects of the Ethanolic Extract of Salix aegyptiaca in Colon Cancer Cells: Involvement of Akt/PKB and MAPK Pathways
Enayat, Shabnam; Ceyhan, Muserref Seyma; BAŞARAN, ARİF AHMET; Gürsel, Mayda; Banerjee, Sreeparna (Informa UK Limited, 2013-10-01)
The bark from Salix species of plants has been traditionally consumed for its antiinflammatory properties. Because inflammation frequently accompanies the progress of colorectal cancer (CRC), we have evaluated the anticancer properties of the ethanolic extract from the bark (EEB) of S. aegyptiaca, a Salix species endogenous to the Middle East, using HCT-116 and HT29 CRC cell lines. Fresh bark from S. aegyptiaca was extracted with ethanol, fractionated by solvent-solvent partitioning and the fractions were a...
Antioxidant and cytotoxic properties of Salvia Absconditiflora and effects on CYP1A1, CYP1B1 gene expressions in breast cancer cell lines
Yılmaz, Selis; İşcan, Mesude; Güray, Tülin; Department of Biochemistry (2013)
Salvia genus is a widely cultivated genus and used in medicine for various purposes as having antimicrobial, antioxidant, anticarcinogen and anti-inflammatory features. In this study the aim was to investigate phenolic composition of Salvia absconditiflora and understand the possible effects of those constituents in cancer related drug metabolizing enzymes. Salvia absconditiflora showed 80,43 % Radical Scavenging Activity against DPPH radical. Total flavonoid content was found as one third of total phenolic...
Comparative effects of emodin on biological activities of MCF-7 and MDA-231 cell lines
Sakallı, Elif; İşcan, Mesude; Department of Biochemistry (2010)
Emodin (1,3,8-trihydroxy-6-methylanthraquinone) is a phytoestrogenic component of Rheum plant extracts which has been used for medical treatment since ancient times. It has been shown to have anti-inflammatory and anti-cancer effects. In our research, we aimed to study the biological effects of emodin on MCF-7 and MDA-231 cell lines. Cytotoxicity assays showed that emodin treatment for 48hours caused a concentration dependent decrease in viable cell numbers of both cell lines. As determined by cell counting...
Antioxidant capacities of Gundelia tournefortii L. extracts and inhibition on glutathione-S-transferase activity
Çoruh, Nursen; Ozgokce, F.; Iscan, M. (Elsevier BV, 2007-01-01)
Gundelia tournefortii L. is an important food source and a well-known medicinal plant in Eastern Anatolia. Therapeutic effects of medicinal plants are known to be closely related to their antioxidant capacities. Antioxidant activities of G. tournefortii, both for the aerial parts and seeds, were investigated by using both 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging and lipid peroxidation inhibition methods. The seeds were found to have higher antioxidant potential than the aerial, with IC50 valu...
Citation Formats
S. Enayat, “Anticarcinogenic effects of the ethanolic extract from salix aegyptiaca l. in colon cancer, involvement of akt pathway,” Ph.D. - Doctoral Program, Middle East Technical University, 2013.