Development of a novel biosensor based on a conducting polymer

Date

2014-01-15

Author

SÖYLEMEZ, SANİYE
Kanik, Fulya Ekiz
Ileri, Merve
Hacioglu, Serife O.
Toppare, Levent Kamil

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A new type of amperometric cholesterol biosensor was fabricated to improve the biosensor characteristics such as sensitivity and reliability. For this purpose, a novel immobilization matrix 2-(4-fluorophenyl)-4,7-di(thiophene-2-yl)-1H-benzo[d]imidazole (BIPF) was electrochemically deposited on a graphite electrode and used as a matrix for the immobilization of cholesterol oxidase (ChOx). Due to strong pi-pi stacking of aromatic groups in the structures of polymer backbone and enzyme molecule, one can easily achieve a sensitive and reliable biosensor without using any membrane or covalent bond formation between the enzyme molecules and polymer surface. Moreover, through pendant fluorine group of the polymer, H-bond formation between with enzyme molecules and polymer was generated. Cholesterol was used as the substrate and amperometric response was measured in correlation with cholesterol amount, at -0.7 V vs. Ag/AgCl in phosphate buffer (pH 7.0). Consequently, optimum conditions for this constructed biosensor were determined. K(M)app, I-max, LOD and sensitivity values were investigated and calculated as 4.0 nM, 2.27 mu A, 0.404 mu M and 1.47 mA/mM cm(2), respectively. A novel and accurate cholesterol biosensor was developed for the determination of total cholesterol in food samples.

Subject Keywords

Conducting polymer, Cholesterol biosensor, Amperometric biosensor, Cholesterol oxidase

URI

https://hdl.handle.net/11511/34791

Journal

TALANTA

DOI

https://doi.org/10.1016/j.talanta.2013.10.007

Collections

Department of Chemistry, Article

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Citation Formats

S. SÖYLEMEZ, F. E. Kanik, M. Ileri, S. O. Hacioglu, and L. K. Toppare, “Development of a novel biosensor based on a conducting polymer,” TALANTA, pp. 84–89, 2014, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/34791.