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Immobilization of invertase and glucose oxidase in conducting copolymers of thiophene functionalized poly(vinyl alcohol) with pyrrole
Date
2006-03-01
Author
Sahmetlioglu, E
Yuruk, H
Toppare, Levent Kamil
Cianga, I
Yagci, Y
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Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License
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In this study, immobilizations of invertase and glucose oxidase were achieved in conducting thiophene functionalized copolymers of vinyl alcohol with thiophene side groups and pyrrole (PVATh/PPy) via electrochemical polymerization. The kinetic parameters, V-max (maximum reaction rate) and K-m (substrate affinity), of both free and immobilized enzymes were determined. The effect of supporting electrolytes, p-toluene sulfonic acid and sodium dodecyl sulfate, on the enzyme activity and film morphologies was examined. The optimum temperature, operational and storage stabilities of immobilized enzymes were determined. PVATh/PPy copolymer was found to exhibit significantly enhanced properties compared to pristine polypyrrole.
Subject Keywords
Materials Chemistry
,
Biochemistry
,
General Chemistry
,
General Chemical Engineering
,
Polymers and Plastics
,
Environmental Chemistry
URI
https://hdl.handle.net/11511/48665
Journal
REACTIVE & FUNCTIONAL POLYMERS
DOI
https://doi.org/10.1016/j.reactfunctpolym.2005.08.009
Collections
Department of Chemistry, Article
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E. Sahmetlioglu, H. Yuruk, L. K. Toppare, I. Cianga, and Y. Yagci, “Immobilization of invertase and glucose oxidase in conducting copolymers of thiophene functionalized poly(vinyl alcohol) with pyrrole,”
REACTIVE & FUNCTIONAL POLYMERS
, pp. 365–371, 2006, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/48665.