Immobilization of invertase and glucose oxidase in conducting copolymers of thiophene functionalized poly(vinyl alcohol) with pyrrole

Date

2006-03-01

Author

Sahmetlioglu, E
Yuruk, H
Toppare, Levent Kamil
Cianga, I
Yagci, Y

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In this study, immobilizations of invertase and glucose oxidase were achieved in conducting thiophene functionalized copolymers of vinyl alcohol with thiophene side groups and pyrrole (PVATh/PPy) via electrochemical polymerization. The kinetic parameters, V-max (maximum reaction rate) and K-m (substrate affinity), of both free and immobilized enzymes were determined. The effect of supporting electrolytes, p-toluene sulfonic acid and sodium dodecyl sulfate, on the enzyme activity and film morphologies was examined. The optimum temperature, operational and storage stabilities of immobilized enzymes were determined. PVATh/PPy copolymer was found to exhibit significantly enhanced properties compared to pristine polypyrrole.

Subject Keywords

Materials Chemistry, Biochemistry, General Chemistry, General Chemical Engineering, Polymers and Plastics, Environmental Chemistry

URI

https://hdl.handle.net/11511/48665

Journal

REACTIVE & FUNCTIONAL POLYMERS

DOI

https://doi.org/10.1016/j.reactfunctpolym.2005.08.009

Collections

Department of Chemistry, Article

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Citation Formats

E. Sahmetlioglu, H. Yuruk, L. K. Toppare, I. Cianga, and Y. Yagci, “Immobilization of invertase and glucose oxidase in conducting copolymers of thiophene functionalized poly(vinyl alcohol) with pyrrole,” REACTIVE & FUNCTIONAL POLYMERS, pp. 365–371, 2006, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/48665.