Crosslinked pullulan/cellulose acetate fibrous scaffolds for bone tissue engineering

Date

2016-12-01

Author

Atila, Deniz
Keskin, Dilek
Tezcaner, Ayşen

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

39
views

0
downloads

Natural polymer based fibrous scaffolds have been explored for bone tissue engineering applications; however, their inadequate 3-dimensionality and poor mechanical properties are among the concerns for their use as bone substitutes. In this study, pullulan (P) and cellulose acetate (CA), two polysaccharides, were electrospun at various P/CA ratios (P-80/CA(20), P-50/CA(50), and F-20/CA(80)%) to develop 3D fibrous network. The scaffolds were then crosslinked with trisodium trimetaphosphate (STMP) to improve the mechanical properties and to delay fast weight loss. The lowest weight loss was observed for the groups that were crosslinked with P/STMP 2/1 for 10 min. Fiber morphologies of P-50/CA(50), were more uniform without phase separation and this group was crosslinked most efficiently among groups. It was found that mechanical properties of P-20/CA(80) and P-50/CA(50) were higher than that of P-80/CA(20). After crosslinking strain values of P-50/CA(50) scaffolds were improved and these scaffolds became more stable. Unlike P-80/CA(20), uncrosslinked P-50/CA(50) and P-20/CA(80) were not lost in PBS. Among all groups, crosslinked P-50/CA(50) scaffolds had more uniform pores; therefore this group was used for bioactivity and cell culture studies. Apatite-like structures were observed on fibers after SBF incubation. Human Osteogenic Sarcoma Cell Line (Saos-2) seeded onto crosslinked P-50/CA(50) scaffolds adhered and proliferated. The functionality of cells was tested by measuring ALP activity of the cells and the results indicated their osteoblastic differentiation. In vitro tests showed that scaffolds were cytocompatible. To sum up, crosslinked P-50/CA(50) scaffolds were proposed as candidate cell carriers for bone tissue engineering applications.

Subject Keywords

Pullulan, Cellulose acetate, Wet electrospinning, Bone tissue engineering

URI

https://hdl.handle.net/11511/36521

Journal

MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS

DOI

https://doi.org/10.1016/j.msec.2016.08.015

Collections

Department of Engineering Sciences, Article

Suggestions

OpenMETU
Core

Synthesis, characterization and osteoblastic activity of polycaprolactone nanofibers coated with biomimetic calcium phosphate MAVİŞ, BORA; Demirtas, Tolga T.; GÜMÜŞDERELİOĞLU, MENEMŞE; Gündüz, Güngör; Colak, Uner (2009-10-01) Immersion of electrospun polycaprolactone (PCL) nanofiber mats in calcium phosphate solutions similar to simulated body fluid resulted in deposition of biomimetic calcium phosphate layer on the nanofibers and thus a highly bioactive novel scaffold has been developed for bone tissue engineering. Coatings with adequate integrity, favorable chemistry and morphology were achieved in less than 6 h of immersion. In the coating solutions, use of lower concentrations of phosphate sources with respect to the literat...
Preparation and characterization of poly(epsilon-caprolactone) scaffolds modified with cell-loaded fibrin gel Malikmammadov, Elbay; Endoğan Tanır, Tuğba; Kızıltay, Aysel; Hasırcı, Nesrin (2019-03-15) Poly(epsilon-caprolactone) (PCL) is one of the most commonly used polymers in the production of tissue engineered scaffolds for hard tissue treatments. Incorporation of cells into these scaffolds significantly enhances the healing rate of the tissue. In this study, PCL scaffolds were prepared by wet spinning technique and modified by addition of fibrinogen in order to form a fibrin network between the PCL fibers. By this way, scaffolds would have micro and nanofibers in their structures. Drying of the wet s...
Cellulose acetate-gelatin-coated boron-bioactive glass biocomposite scaffolds for bone tissue engineering Rad, Reza Moonesi; ALSHEMARY, AMMAR ZEIDAN GHAILAN; Evis, Zafer; Keskin, Dilek; Tezcaner, Ayşen (IOP Publishing, 2020-11-01) In this study, we aimed to prepare and characterize porous scaffolds composed of pure and boron oxide (B2O3)-doped bioactive glass (BG) that were infiltrated by cellulose acetate-gelatin (CA-GE) polymer solution for bone tissue engineering applications. Composite scaffolds were cross-linked with glutaraldehyde after polymer coating to protect the structural integrity of the polymeric-coated scaffolds. The impact of B(2)O(3)incorporation into BG-polymer porous scaffolds on the cross-sectional morphology, por...
Silicate-doped nano-hydroxyapatite/graphene oxide composite reinforced fibrous scaffolds for bone tissue engineering Dalgıç, Ali Deniz; Tezcaner, Ayşen; Keskin, Dilek; Evis, Zafer (SAGE Publications, 2018-3-15) In this study, novel graphene oxide-incorporated silicate-doped nano-hydroxyapatite composites were prepared and their potential use for bone tissue engineering was investigated by developing an electrospun poly(epsilon-caprolactone) scaffold. Nanocomposite groups were synthesized to have two different ratios of graphene oxide (2 and 4 wt%) to evaluate the effect of graphene oxide incorporation and groups with different silicate-doped nano-hydroxyapatite content was prepared to investigate optimum concentra...
Fabrication and characterization of bilayered tissue scaffolds incorporating bioactive agents for skin tissue engineering applications Aktürk, Ömer; Keskin, Dilek; Bilici, Temel; Department of Engineering Sciences (2015) In this study, it was aimed to fabricate tissue scaffolds from different biological polymers (collagen, silk fibroin and sericin) for skin tissue engineering applications. For this purpose, bilayered scaffolds composed of epidermal (collagen/sericin films) and dermal (collagen sponges, collagen matrices or silk fibroin matrices) layers were produced with different biomaterial fabrication methods. Casting and solvent evaporation (film), lyophilization/freeze-drying (sponge) and dry/wet electro-spinning (micr...

Citation Formats

D. Atila, D. Keskin, and A. Tezcaner, “Crosslinked pullulan/cellulose acetate fibrous scaffolds for bone tissue engineering,” MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS, pp. 1103–1115, 2016, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/36521.