Date

2022-5-27

Author

Nosratinia, Ataollah

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Poly (ether ether ketone) (PEEK) has attracted the interest of bone tissue engineers due to its close range of mechanical properties to that of human bones, its biocompatibility, and excellent chemical resistance. However, PEEK is bioinert and has very low hydrophilicity. Sulfonation can compensate for this by introducing hydrophilic sulfonate groups. Furthermore, hydroxyapatite (HA) which is the main component of the bone, can introduce bioactivity to the scaffold. Compared to pure HA, Fluoridated hydroxyapatite (FHA) has higher stability and possesses higher osteogenic activity. In this context, FHA was prepared and combined with previously prepared Sulfonated PEEK (sPEEK). Wet-electrospinning method was used to make sPEEK scaffolds combined with HA and FHA in 1%, 2.5%, and 5% ratios for bone tissue engineering. SEM Analysis, FTIR Analysis, Mechanical Strength tests, Water uptake measurement, in vitro degradation assay, Simulated Body Fluid assay, and cell attachment and proliferation assay were performed. Even though toxic at high concentrations, HA and FHA at ratios that were combined with sPEEK to form electrospun scaffolds showed good cell viability and improved proliferation at the optimum concentrations. Scaffolds show mineralization in the SBF solution and tend to gradually degrade in PBS. In vitro experiments with Saos-2 cells show that FHA in optimum ratios is less cytotoxic than HA and incorporation of both HA and FHA in optimum ratios into the sPEEK scaffolds enhance cell proliferation. Among groups, considering all properties 1% FHA*sPEEK can be suggested as the most promising one. It is concluded that the scaffolds are cytocompatible and provide a desirable environment for cell growth and proliferation.

Subject Keywords

Bone tissue engineering, sPEEK, Fluorohydroxyapatite, Electrospinning

URI

https://hdl.handle.net/11511/98160

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Graduate School of Natural and Applied Sciences, Thesis