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Production of xylooligosaccharides from cotton and tobacco stalk

Akpinar, Ozlem
Erdogan, Kader
Ak, Ozlem
Bakir, Ufuk
Yılmaz, Levent
Xylooligosaccharides (XOs) are non digestible oligosaccharides which have many beneficial health effects. XOs are produced from xylan rich materials. For production of XOs, among the xylan rich materials, the residual origin such as forestal, agricultural or industrial wastes of lignocellulosic nature were special interest since utilization agricultural biomass as raw material provides economic and ecological benefits. The driving force of this study was to test the suitability of cotton and tobacco stalks for production of XO. These are the residue remaining after the harvest. After cotton and tobacco stalks were dried and grounded, their chemical structure were characterized. After xylan was extracted from cotton and tobacco stalk with alkali, they were converted into XOs with enzymatic process. The hydrolysis condition was optimized by using different temperatures, substrate and enzyme concentrations. The results showed that XOs concentration increased as the reaction times increased. XOs concentration was characterized by a rapid increase in the reducing sugar depending on the substrate concentration. The time XOs production was shortened depending on enzyme concentrations. The xylan was hydrolyzed to a variety of oligosaccharides, xylopentaose and xylohexose being the major products followed by xlobiose and xylotriose. This study demostrated that cotton and tobacco stalk, which has no economical value, could be easily converted to more valuable product, XOs, by enzyme and these oligosaccharides could be used for further application.