A THERMO-STABLE DEHALOGENASE IN THE EXTRACTS FROM PSEUDOMONAS SP (STRAIN-19S)

Date

1989-06-01

Author

Kocabıyık, Semra

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A thermo-stable dehalogenase was demonstrated in the crude extracts from Pseudomonas sp (19S). The ability of the enzyme to catalyze the dehalogenation of various halogen-substituted organic acids was investigated and the highest activity was found with monochloroacetate. The enzyme followed Michael is-Menten kinetics, and the Km for monochloroacetate was O.2mM. Maximum activity was found at pH 10.5 and 60~ The enzyme activity in the cell-free extract was unaffected by EDTA or by Mn, Zn, or Cu ions, but was dramatically reduced by HgClz (70 %) and Pb (N03)~ (80 %).

Subject Keywords

Biotechnology, General Medicine

URI

https://hdl.handle.net/11511/37210

Journal

BIOTECHNOLOGY LETTERS

DOI

https://doi.org/10.1007/bf01089471

Collections

Department of Biology, Article

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Citation Formats

S. Kocabıyık, “A THERMO-STABLE DEHALOGENASE IN THE EXTRACTS FROM PSEUDOMONAS SP (STRAIN-19S),” BIOTECHNOLOGY LETTERS, pp. 397–400, 1989, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/37210.