A thermo-stable dehalogenase was demonstrated in the crude extracts from Pseudomonas sp (19S). The ability of the enzyme to catalyze the dehalogenation of various halogen-substituted organic acids was investigated and the highest activity was found with monochloroacetate. The enzyme followed Michael is-Menten kinetics, and the Km for monochloroacetate was O.2mM. Maximum activity was found at pH 10.5 and 60~ The enzyme activity in the cell-free extract was unaffected by EDTA or by Mn, Zn, or Cu ions, but was dramatically reduced by HgClz (70 %) and Pb (N03)~ (80 %).


Production of neutral and alkaline extracellular proteases by the thermophilic fungus, Scytalidium thermophilum, grown on microcrystalline cellulose
Ifrij, IH; Ögel, Zümrüt Begüm (Springer Science and Business Media LLC, 2002-07-01)
Extracellular proteases produced by Scytalidium thermophilum, grown on microcrystalline cellulose, were most active at pH 6.5-8 and 37-45 degreesC when incubated for 60 min. Highest protease activity was at day 3 where endoglucanase activity was low. Protease activity measurements with and without the protease inhibitors, p-chloromercuribenzoate, PMSF, antipain, E-64, EDTA and pepstatin A, suggest production of thiol-containing serine protease and serine proteases. Endoglucanase and Avicel-adsorbable endogl...
AFKHAMI, P; Özcengiz, Gülay; ALAEDDINOGLU, NG (Springer Science and Business Media LLC, 1993-12-01)
A local isolate of Bacillus thuringiensis (B.t.81) produced crystal protein was identified as a cry I gene of Class I. The synthesis and assembly of crystal complements were investigated at intervals throughout the growth cycle. Incubation temperature had a marked effect on toxin synthesis; production being the highest at 25-degrees-C and the lowest at 42-degrees-C. The mutants of B. t. 81, unable to synthesize crystal protein complements, have also been described.
Effects of pulse feeding of beet molasses on recombinant benzaldehyde lyase production by Escherichia coli BL21(DE3)
Çalık, Pınar (Springer Science and Business Media LLC, 2009-11-01)
The effect of fed-batch operation (FBO) strategy was investigated using pretreated-beet molasses, containing galactose that induces the lac promoter, on benzaldehyde lyase (BAL) production by recombinant Escherichia coli BL21(DE3)pLySs. After batch cultivation with 30 g l(-1) pretreated-beet molasses consisting of 7.5 g l(-1) glucose and 7.5 g l(-1) fructose, three FBO strategies were applied at dissolved oxygen (=40%) cascade to air-flow rate. In FBO1 when air-flow rate decreased considerably, feed was giv...
Selective oxidation and reduction reactions with cofactor regeneration mediated by galactitol-, lactate-, and formate dehydrogenases immobilized on magnetic nanoparticles
Demir, Ayhan Sıtkı; Talpur, Farah N.; Sopaci, S. Betul; Kohring, Gert-W; ÇELİK, AYHAN (Elsevier BV, 2011-04-10)
Rapid immobilization with the one-pot purification of galactitol dehydrogenase (GatDH) and formate dehydrogenase (FDH) is achieved by using iminodiacetic acid (IDA) with chelated Co2+ modified magnetic nanoparticles as a carrier. Lactate dehydrogenase (LDH) from recombinant Escherichia coli and FDH commencing Candida methylica were used as an auxiliary enzyme for the regeneration of NADH/NAD(+) with a representative synthesis of (S)-1,2-propanediol and L-tagatose starting from hydroxyacetone and galactitol....
Isolation of antimicrobial molecules from agricultural biomass and utilization in xylan-based biodegradable films
Çekmez, Umut; Bakır, Ufuk; Department of Biotechnology (2010)
Cotton stalk lignin extractions were performed via alkaline methods at different conditions. Crude and post treated cotton stalk lignins, olive mill wastewater and garlic stalk juice were examined in terms of antimicrobial activity. Antimicrobial lignin was isolated depending on alkaline extraction conditions. Lignin extracted at 60°C exhibited significant antimicrobial effect towards both Escherichia coli and Bacillus pumilus. However different post treatments such as ultrasonication and TiO2-assisted phot...
Citation Formats
S. Kocabıyık, “A THERMO-STABLE DEHALOGENASE IN THE EXTRACTS FROM PSEUDOMONAS SP (STRAIN-19S),” BIOTECHNOLOGY LETTERS, pp. 397–400, 1989, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/37210.