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A THERMO-STABLE DEHALOGENASE IN THE EXTRACTS FROM PSEUDOMONAS SP (STRAIN-19S)
Date
1989-06-01
Author
Kocabıyık, Semra
Turkoglu, S
Metadata
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This work is licensed under a
Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License
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A thermo-stable dehalogenase was demonstrated in the crude extracts from Pseudomonas sp (19S). The ability of the enzyme to catalyze the dehalogenation of various halogen-substituted organic acids was investigated and the highest activity was found with monochloroacetate. The enzyme followed Michael is-Menten kinetics, and the Km for monochloroacetate was O.2mM. Maximum activity was found at pH 10.5 and 60~ The enzyme activity in the cell-free extract was unaffected by EDTA or by Mn, Zn, or Cu ions, but was dramatically reduced by HgClz (70 %) and Pb (N03)~ (80 %).
Subject Keywords
Biotechnology
,
General Medicine
URI
https://hdl.handle.net/11511/37210
Journal
BIOTECHNOLOGY LETTERS
DOI
https://doi.org/10.1007/bf01089471
Collections
Department of Biology, Article