A sepiolite modified conducting polymer based biosensor

2013-11-01
SÖYLEMEZ, SANİYE
Kanik, Fulya Ekiz
Tarkuc, Simge
UDUM, YASEMİN
Toppare, Levent Kamil
A conducting polymer modified with sepiolite was utilized in the construction of a highly sensitive and fast amperometric cholesterol biosensor. In this study a monomer; (10,13-bis(2,3-dihydrothieno[3,4-b][1,4]dioxin-5-yl)dibenzo[a,c]phenazine (PHED)) was synthesized and then its polymer was coated on a graphite electrode by electropolymerization to obtain a matrix for enzyme immobilization. Cholesterol oxidase was immobilized onto polymer coated electrode by adsorption technique. Sepiolite was introduced for a successful immobilization of the cholesterol oxidase. Immobilized enzyme kinetic parameters (K-M(app), I-max) were evaluated by Michaelis-Menten kinetics and calculated as 0.031 mM and 6.06 mu A, respectively. LOD and sensitivity were estimated as 0.36 mu M and 1.64 mA/mMcm(2). Characterization of designed biosensor was done to examine the effect of various factors such as enzyme amount, optimum pH and shelf-life. A novel accurate and inexpensive cholesterol biosensor was developed for the determination of total cholesterol in food samples.
COLLOIDS AND SURFACES B-BIOINTERFACES

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Citation Formats
S. SÖYLEMEZ, F. E. Kanik, S. Tarkuc, Y. UDUM, and L. K. Toppare, “A sepiolite modified conducting polymer based biosensor,” COLLOIDS AND SURFACES B-BIOINTERFACES, pp. 549–555, 2013, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/40212.