A sepiolite modified conducting polymer based biosensor

Kanik, Fulya Ekiz
Tarkuc, Simge
Toppare, Levent Kamil
A conducting polymer modified with sepiolite was utilized in the construction of a highly sensitive and fast amperometric cholesterol biosensor. In this study a monomer; (10,13-bis(2,3-dihydrothieno[3,4-b][1,4]dioxin-5-yl)dibenzo[a,c]phenazine (PHED)) was synthesized and then its polymer was coated on a graphite electrode by electropolymerization to obtain a matrix for enzyme immobilization. Cholesterol oxidase was immobilized onto polymer coated electrode by adsorption technique. Sepiolite was introduced for a successful immobilization of the cholesterol oxidase. Immobilized enzyme kinetic parameters (K-M(app), I-max) were evaluated by Michaelis-Menten kinetics and calculated as 0.031 mM and 6.06 mu A, respectively. LOD and sensitivity were estimated as 0.36 mu M and 1.64 mA/mMcm(2). Characterization of designed biosensor was done to examine the effect of various factors such as enzyme amount, optimum pH and shelf-life. A novel accurate and inexpensive cholesterol biosensor was developed for the determination of total cholesterol in food samples.


A biosensor platform based on amine functionalized conjugated benzenediamine-benzodithiophene polymer for testosterone analysis
BULUT, Umut; ŞANLI, SERDAR; Cevher, Sevki Can; Çırpan, Ali; Donmez, Sude; TİMUR, SUNA (Wiley, 2020-11-01)
A novel benzenediamine‐benzodithiophene polymer is synthesized for use in biosensor fabrication for the detection of testosterone. The sensory platform is constructed via drop coating on a screen‐printed carbon electrode, using poly(benzenediamine‐Bis[(2‐ethylhexyl)oxy]benzodithiophene) (pBDBT) as the polymer layer. Testosterone antibodies are immobilized on the polymer‐coated electrode surface via glutaraldehyde, which binds to the surface through the amino functional groups on the polymer backbone. The ch...
Kocabıyık, Semra (Springer Science and Business Media LLC, 1989-06-01)
A thermo-stable dehalogenase was demonstrated in the crude extracts from Pseudomonas sp (19S). The ability of the enzyme to catalyze the dehalogenation of various halogen-substituted organic acids was investigated and the highest activity was found with monochloroacetate. The enzyme followed Michael is-Menten kinetics, and the Km for monochloroacetate was O.2mM. Maximum activity was found at pH 10.5 and 60~ The enzyme activity in the cell-free extract was unaffected by EDTA or by Mn, Zn, or Cu ions, but was...
Addressing challenges in the ultrafiltration of biomolecules from complex aqueous environments
Büküşoğlu, Emre; Koku, Harun; Çulfaz Emecen, Pınar Zeynep (Elsevier BV, 2020-04-01)
Substantial progress has been made in ultrafiltration processes toward the separation of colloidal species from aqueous environments. Studies, especially in the past decade, have advanced the separations using functional and nano-structured membrane materials and a thorough understanding of colloid-membrane and colloid-colloid interactions in aqueous media. In this context, this review describes the recent advances in ultrafiltration of biomolecular species, most specifically proteins, from complex environm...
A newly designed anthracene and isoindigo based polymer: synthesis, electrochemical characterization and biosensor applications
Soylemez, Saniye; Goker, Seza; Toppare, Levent Kamil (Royal Society of Chemistry (RSC), 2019-09-21)
A glucose biosensor based on a newly designed and synthesized polymer, poly[(E)-6-methyl-6 '-(10-methylanthracen-9-yl)-1,1 '-diundecyl-[3,3 '-biindolinylidene]-2,2 '-dione] (namely PIIDAnth), and single walled carbon nanotubes (SWCNTs) was fabricated via an easy one pot electrode preparation and explored as a glucose electrochemical sensor. The key aim of this paper is to emphasize creation of the new surface design of sensing platforms based on a novel polymer and nanomaterial for glucose detection. With t...
A bio-sensing platform utilizing a conjugated polymer, carbon nanotubes and PAMAM combination
Buber, Ece; Kesik, Melis; Soylemez, Saniye; Toppare, Levent Kamil (Elsevier BV, 2017-08-15)
In this study, amperometric biosensing performance of a novel platform utilizing a conducting polymer (CP), multi-walled carbon nanotubes (MWCNTs) and poly(amidoamine) (PAMAM) dendrimer was constructed. In order to fabricate the proposed biosensor, a graphite electrode was modified with poly[9,9-di-(2-ethylhexyl)-fluorenyl-2,7-diyl] end capped with N,N-bis(4-methylphenyl)-4-aniline (PFLA), MWCNTs and PAMAM, respectively. Then, GOx was immobilized onto the modified surface with the help of glutaraldehyde (GA...
Citation Formats
S. SÖYLEMEZ, F. E. Kanik, S. Tarkuc, Y. UDUM, and L. K. Toppare, “A sepiolite modified conducting polymer based biosensor,” COLLOIDS AND SURFACES B-BIOINTERFACES, pp. 549–555, 2013, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/40212.