A sepiolite modified conducting polymer based biosensor

Date

2013-11-01

Author

SÖYLEMEZ, SANİYE
Kanik, Fulya Ekiz
Tarkuc, Simge
UDUM, YASEMİN
Toppare, Levent Kamil

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A conducting polymer modified with sepiolite was utilized in the construction of a highly sensitive and fast amperometric cholesterol biosensor. In this study a monomer; (10,13-bis(2,3-dihydrothieno[3,4-b][1,4]dioxin-5-yl)dibenzo[a,c]phenazine (PHED)) was synthesized and then its polymer was coated on a graphite electrode by electropolymerization to obtain a matrix for enzyme immobilization. Cholesterol oxidase was immobilized onto polymer coated electrode by adsorption technique. Sepiolite was introduced for a successful immobilization of the cholesterol oxidase. Immobilized enzyme kinetic parameters (K-M(app), I-max) were evaluated by Michaelis-Menten kinetics and calculated as 0.031 mM and 6.06 mu A, respectively. LOD and sensitivity were estimated as 0.36 mu M and 1.64 mA/mMcm(2). Characterization of designed biosensor was done to examine the effect of various factors such as enzyme amount, optimum pH and shelf-life. A novel accurate and inexpensive cholesterol biosensor was developed for the determination of total cholesterol in food samples.

Subject Keywords

Biotechnology, Physical and Theoretical Chemistry, Colloid and Surface Chemistry, Surfaces and Interfaces, General Medicine

URI

https://hdl.handle.net/11511/40212

Collections

Department of Chemistry, Article