Show/Hide Menu
Hide/Show Apps
anonymousUser
Logout
Türkçe
Türkçe
Search
Search
Login
Login
OpenMETU
OpenMETU
About
About
Açık Bilim Politikası
Açık Bilim Politikası
Frequently Asked Questions
Frequently Asked Questions
Browse
Browse
By Issue Date
By Issue Date
Authors
Authors
Titles
Titles
Subjects
Subjects
Communities & Collections
Communities & Collections
Boron-doped Biphasic Hydroxyapatite/beta-Tricalcium Phosphate for Bone Tissue Engineering
Date
2020-06-10
Author
Pazarçeviren, Ahmet Engin
Tezcaner, Ayşen
Keskin, Dilek
Kolukisa, Serap Topsoy
Surdem, Sedat
Evis, Zafer
Metadata
Show full item record
This work is licensed under a
Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License
.
Item Usage Stats
11
views
0
downloads
Boron-doped hydroxyapatite/tricalcium phosphates (BHTs) were synthesized to study boron uptake and correlate structural alterations of incremental boron addition (0 to 10 mol%). BHTs with a Ca/P ratio of 1.6 were prepared by a wet precipitation/microwave reflux method, sieved (< 70 mu m) and characterized. XRD and FTIR analyses revealed that boron slightly distorted apatite crystal, increased crystallinity (95.78 +/- 2.08% for 5BHT) and crystallite size (103.39 +/- 23.47 nm for 5BHT) and still, boron addition did not show any further detrimental effects. Total surface area (4.05 +/- 0.82 m(2)/g for 10BHT) and mesoporosity (23.90 +/- 7.92 mu L/g for 10BHT) were expanded as boron content was increased. Moreover, boron addition made grains become smaller (0.21 +/- 0.06 mu m for 5BHT) and ordered while hardness (10.51 +/- 0.86 GPa for 10BHT) increased. Boron incorporation enhanced bioactivity with significantly highest calcium phosphate deposition and protein adsorption (135.29 +/- 29.58 mu g on 10BHT). In return, boron favored highest alkaline phosphatase activity (4.80 +/- 0.40 M-ALP/ng(DNA).min), intracellular calcium (23.61 +/- 0.68 g/g(DNA)), phosphate (31.84 +/- 4.68 g/g(DNA)), and protein (23.70 +/- 3.46 g/g(DNA)) storage in 5BHT without cytotoxicity (128 +/- 18% viability compared to pure HT). Compared to literature, it can be pointed out that we successfully employed an optimal procedure for production of BHTs and incorporated significantly higher boron content in HT (5.23 mol%). Additionally, results tended to conclude that 5BHT samples (5 mol% boron in HT) demonstrated a very high potential to be used in composite bone tissue constructs.
Subject Keywords
Inorganic Chemistry
,
Clinical Biochemistry
,
Biochemistry
,
Endocrinology, Diabetes and Metabolism
,
Biochemistry, medical
,
General Medicine
URI
https://hdl.handle.net/11511/40751
Journal
BIOLOGICAL TRACE ELEMENT RESEARCH
DOI
https://doi.org/10.1007/s12011-020-02230-8
Collections
Department of Engineering Sciences, Article