3D Printed Hydrogel Multiassay Platforms for Robust Generation of Engineered Contractile Tissues

Christensen, Rie Kjaer
Laier, Christoffer von Halling
Kızıltay, Aysel
Wilson, Sandra
Larsen, Niels Bent
We present a method for reproducible manufacture of multiassay platforms with tunable mechanical properties for muscle tissue strip analysis. The platforms result from stereolithographic 3D printing of low protein-binding poly(ethylene glycol) diacrylate (PEGDA) hydrogels. Contractile microtissues have previously been engineered by immobilizing suspended cells in a confined hydrogel matrix with embedded anchoring cantilevers to facilitate muscle tissue strip formation. The 3D shape and mechanical properties of the confinement and the embedded cantilevers are critical for the tissue robustness. High-resolution 3D printing of PEGDA hydrogels offers full design freedom to engineer cantilever stiffness, while minimizing unwanted cell attachment. We demonstrate the applicability by generating suspended muscle tissue strips from C2C12 mouse myoblasts in a compliant fibrin-based hydrogel matrix. The full design freedom allows for new platform geometries that reduce local stress in the matrix and tissue, thus, reducing the risk of tissue fracture.


Thermal and dynamic mechanical properties of microwave and heat-cured poly(methyl methacrylate) used as dental base material
Muhtarogullari, IY; Dogan, A; Muhtarogullari, M; Usanmaz, Ali (Wiley, 1999-12-13)
In this study, the particle size distribution, molecular weight, thermal analysis (TGA) differential scanning calorimetry (DSC) and thermogravimetric analysis, and dynamic mechanical analysis (DMA) of poly(methyl methacrylate) used as dental base material were investigated. The commercial raw material used were prepared for microwave curing, and they were cured by microwave and conventional heat methods. The average particle size of the powder studied (103.1 mu m) were much larger than that of the commercia...
Multifunctional layer-by-layer modified chitosan/poly(ethylene glycol) hydrogels
Onat, Bora; Ulusan, Sinem; Banerjee, Sreeparna; Erel Göktepe, İrem (Elsevier BV, 2019-03-01)
We report the surface modification of chitosan/poly(ethylene glycol) (chitosan/PEG) hydrogel materials via layer-by-layer (LbL) technique using stimuli-responsive polymers. Water-soluble complexes of Tannic Acid (TA) and a broad-spectrum antibiotic, Ciprofloxacin (Cipro) were prepared and co-assembled at the surface of Chitosan/PEG hydrogels with poly(N-vinyl caprolactam) (PVCL). Compared to the bare hydrogels, the surface spreading and proliferation of human fibroblasts were significantly enhanced on preca...
Dynamic mechanical properties of dental base material reinforced with glass fiber
Karacaer, O; Dogan, A; Dogan, OM; Usanmaz, Ali (Wiley, 2002-08-22)
Dynamic mechanical properties of poly(methyl methacrylate) (PMMA) reinforced with glass fibers were investigated. The composite used as dental base material contained other additives for different purposes to give the same appearance as natural dentals. The fiber-polymer bonding and orientation of fibers in polymer matrix are very important in the application of the product. To see that, the fibers were treated with silane and compared with untreated fibers. The commercial polymer and monomer used were the ...
Poly(methyl methacrylate) hybrid syntactic foams with hollow glass microspheres and polyhedral oligomeric silsesquioxanes
Ozkutlu, Merve; Dilek Hacıhabiboğlu, Çerağ; Bayram, Göknur (Wiley, 2019-08-10)
The study aims to produce poly(methyl methacrylate) (PMMA)-based lower density syntactic foams with hollow glass microspheres (HGMs) and to improve their mechanical properties by the addition of polyhedral oligomeric silsesquioxanes (POSSs) while maintaining the thermal properties of the neat polymer. First to understand the effect of POSS addition, PMMA-POSS composites with octaisobutyl and octaphenyl POSS were produced through extrusion. Higher relative flexural and impact strengths were obtained with POS...
Studies on the modification of interphase/interfaces by use of plasma in certain polymer composite systems
Akovali, G; Dilsiz, N (Wiley, 1996-04-01)
Calcium carbonate and carbon fiber surfaces were modified by use of a series of plasma polymers at different selected plasma conditions, and the effect of surface modification, mainly on the mechanical properties of composite systems prepared, was investigated. The matrices for the composite systems employed were polypropylene and epoxy, for the chalk and C fiber, respectively. Mechanical and thermal studies and scanning electron microscopy (SEM) pictures revealed that inclusion surfaces, being independent ...
Citation Formats
R. K. Christensen, C. v. H. Laier, A. Kızıltay, S. Wilson, and N. B. Larsen, “3D Printed Hydrogel Multiassay Platforms for Robust Generation of Engineered Contractile Tissues,” BIOMACROMOLECULES, pp. 356–365, 2020, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/41020.