A novel functional conducting polymer: synthesis and application to biomolecule immobilization

Kanik, Fulya Ekiz
Rende, Eda
Toppare, Levent Kamil
A recently synthesized conducting polymer poly(TBT6-NH2); poly(6-(4,7-di(thiophen-2-yl)-2H-benzo [d][1,2,3]triazol-2-yl)hexan-1-amine) was utilized as a matrix for biomolecule immobilization. After successful electrochemical deposition the polymer poly(TBT6-NH2) on the graphite electrodes, immobilization of choline oxidase (ChO) was carried out. Due to the free amino functional groups of the polymeric structure, ChO molecules were successfully immobilized onto the polymer surface via covalent binding. For this, glutaraldehyde (GA) was used as crosslinker and bifunctional agent. Hence, a robust binding between the support and the protein molecules was achieved. Scanning electron microscopy (SEM) and X-ray photoelectron spectroscopy (XPS) were used to monitor the surface morphologies of both the polymer and the bioactive layer and to confirm the binding of the protein. Amperometric measurements were recorded by monitoring oxygen consumption in the presence of choline as the substrate at -0.7 V. The optimized biosensor showed a very good linearity between 0.1 and 10 mM with a 7 s response time and a detection limit (LOD) of 16.8 mu M to choline. Also, kinetic parameters, operational and storage stabilities were determined. Finally, designed system was applied for pesticide detection.


A novel approach for the fabrication of a flexible glucose biosensor: The combination of vertically aligned CNTs and a conjugated polymer
GOKOGLAN, Tugba Ceren; SOYLEMEZ, Saniye; KESİK, Melis; DOGRU, Itir Bakis; TUREL, Onur; YÜKSEL, Recep; Ünalan, Hüsnü Emrah; Toppare, Levent Kamil (2017-04-01)
A novel flexible glucose biosensor using vertically aligned carbon nanotubes (VACNT) and a conjugated polymer (CP) was fabricated. A scaffold based on VACNT grown on aluminum foil (VACNT-Al foil) with poly (9,9-di-(2-ethylhexyl)-fluorenyl-2,7-diyl)-end capped with 2,5-diphenyl-1,2,4-oxadiazole (PFLO) was used as the immobilization matrix for the glucose biosensor. Glucose oxidase (GOx) was immobilized on a modified indium tin oxide (ITO) coated polyethylene terephthalate (PET) electrode surface. The biosens...
An acetylcholinesterase biosensor based on a conducting polymer using multiwalled carbon nanotubes for amperometric detection of organophosphorous pesticides
Kesik, Melis; Kanik, Fulya Ekiz; Turan, Janset; Kolb, Marit; TİMUR, SUNA; Bahadir, Muefit; Toppare, Levent Kamil (2014-12-15)
A novel amperometric biosensor based on a conducting polymer using multi walled carbon nanotube modified electrode was developed for detection of organophosphorus pesticides. Acetylcholinesterase (AChE) was successfully immobilized by covalent linkage on the modified graphite electrode. Carbon nanotubes were functionalized by electrochemical treatment. A conducting polymer; poly(4-( 2,5-di(thiophen-2-yl)-1H-pyrrol-1-yl)benzenamine) (poly( SNS-NH2)) was synthesized via electropolymerization to examine its ma...
An amperometric acetylcholine biosensor based on a conducting polymer
Kanik, Fulya Ekiz; Kolb, Marit; TİMUR, SUNA; Bahadir, Muefit; Toppare, Levent Kamil (Elsevier BV, 2013-08-01)
An amperometric acetylcholine biosensor was prepared by the generation of the conducting polymer poly(4-(2,5-di(thiophen-2-yl)-1H-pyrrol-1-yl)benzenamine) (poly(SNS-NH2)) on graphite electrodes. For pesticide detection, the enzymes acetylcholinesterase (AChE) and choline oxidase (ChO) were co-immobilized onto the conducting polymer poly(SNS-NH2) films using covalent binding technique. Electrochemical polymerization was carried out using a three-electrode cell configuration via cyclic voltammetry. Characteri...
A conducting polymer and a calixarene derivative A novel surface design for glucose detection
Gökoğlan, Ceren; Söylemez, Saniye; Kesik, Melis; Ünay, Hande; Sayın, Serkan; Çırpan, Ali; Yıldız, Hüseyin Bekir; Toppare, Levent Kamil (null; 2016-07-17)
In this study, a novel amperometric glucose biosensor based on a conducting polymer and a calixarene was developed. Conducting polymer of (2‐(2‐oc‐tyldodecyl)‐4,7‐di(selenoph‐2‐yl)‐2H‐benzo[d][1,2,3]triazole)) (SBTz) was used as the immobilization matrix for biomolecule deposition to achieve an effective surface design to detect glucose. After successful deposition of SBTz on graphite electrode, a newly synthesized calixarene and gold nanoparticle (AuNP) mixture were used for improving biosensor character...
A novel electrochromic and fluorescent polythienylpyrrole bearing 1,1 '-bipyrrole
CİHANER, ATİLLA; Mert, Olcay; Demir, Ayhan Sıtkı (Elsevier BV, 2009-01-30)
A novel conducting polymer was successfully synthesized via electropolymerization of 1-(1H-pyrrol-1-yl)-2,5-di(thiophen-2-yl)-1H-pyrrole. The electrochemical and electro-optical properties of the corresponding polymer, which was the first example of polymer containing 1,1'-bipyrrole units, were elaborated using electroanalytical and spectroscopic techniques. Cyclic voltammograms and electro-optical studies showed that the polymer has a stable and well-defined reversible redox process as well as electrochrom...
Citation Formats
F. E. Kanik, E. Rende, S. TİMUR, and L. K. Toppare, “A novel functional conducting polymer: synthesis and application to biomolecule immobilization,” JOURNAL OF MATERIALS CHEMISTRY, pp. 22517–22525, 2012, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/41234.