Target-specific delivery of doxorubicin to human glioblastoma cell line via ssDNA aptamer

Akca, Oya Ercan
Eyidogan, Fusun Inci
Öktem, Hüseyin Avni
Targeted drug delivery approaches have been implementing significant therapeutic gain for cancer treatment since last decades. Aptamers are one of the mostly used and highly selective targeting agents for cancer cells. Herein, we address a nano-sized targeted drug delivery approach adorned with A-172 glioblastoma cell-line-specific single stranded DNA (ssDNA) aptamer in which the chemotherapeutic agent Doxorubicin (DOX) had been conjugated. DNA aptamer, GMT-3, was previously selected for specific recognition of glioblastoma and represented many advantageous characteristics for drug targeting purposes. Flow cytometry analysis proved the binding efficiency of the specific aptamer to tumour cell lines. Cell-type-specific toxicity of GMT-3:DOX complex was showed by XTT assay and terminated cytotoxic effects were screened for both target cell and a control breast cancer cell line. The result of this contribution demonstrated the potential utility of GMT-3 aptamer-mediated therapeutic drug transportation in the treatment of gliomas specifically. It was concluded that aptamer-mediated drug delivery can be applied successfully for clinical use.


Exploiting evolutionary steering to induce collateral drug sensitivity in cancer
Acar, Ahmet; Nichol, Daniel; Fernandez-Mateos, Javier; Cresswell, George D.; Barozzi, Iros; Hong, Sung Pil; Trahearn, Nicholas; Spiteri, Inmaculada; Stubbs, Mark; Burke, Rosemary; Stewart, Adam; Caravagna, Giulio; Werner, Benjamin; Vlachogiannis, Georgios; Maley, Carlo C.; Magnani, Luca; Valeri, Nicola; Banerji, Udai; Sottoriva, Andrea (Springer Science and Business Media LLC, 2020-4-21)
Drug resistance mediated by clonal evolution is arguably the biggest problem in cancer therapy today. However, evolving resistance to one drug may come at a cost of decreased fecundity or increased sensitivity to another drug. These evolutionary trade-offs can be exploited using 'evolutionary steering' to control the tumour population and delay resistance. However, recapitulating cancer evolutionary dynamics experimentally remains challenging. Here, we present an approach for evolutionary steering based on ...
Impaired toll like receptor-7 and 9 induced immune activation in chronic spinal cord injured patients contributes to immune dysfunction
Gucluler, Gozde; Adiguzel, Emre; Gungor, Bilgi; Kahraman, Tamer; Gürsel, Mayda; Yilmaz, Bilge; GÜRSEL, İHSAN (Public Library of Science (PLoS), 2017-02-07)
Reduced immune activation or immunosuppression is seen in patients withneurological diseases. Urinary and respiratory infections mainly manifested as septicemia and pneumonia are the most frequent complications following spinal cord injuries and they account for the majority of deaths. The underlying reason of these losses is believed to arise due to impaired immune responses to pathogens. Here, we hypothesized that susceptibility to infections of chronic spinal cord injured (SCI) patients might be due to i...
Improvement of a liposomal formulation with a native molecule: calcitriol
Dalgıç, Ali Deniz; Tezcaner, Ayşen; ARPACI, Fikret; AVCU, Ferit; Keskin, Dilek (Royal Society of Chemistry (RSC), 2016-01-01)
Many studies have been conducted to improve liposome's potency towards cancer treatment. Recently, modifications of liposomal formulations turned towards either dual loading of anticancer agents to minimize the dose of each agent or the use of targeting moieties to target them to or near cancer cells for minimizing side effects with maximum treatment. In this study, a natural molecule, calcitriol, was used to improve the effectiveness of doxorubicin loaded liposomes by co-loading approach. Calcitriol treatm...
DNA Walker-Regulated Cancer Cell Growth Inhibition
Li, Feiran; Cha, Tae-Gon; Pan, Jing; Özçelikkale, Altuğ; Han, Bumsoo; Choi, Jong Hyun (Wiley, 2016-06-01)
We demonstrate a DNAzyme-based walker system as a controlled oligonucleotide drug AS1411 release platform for breast cancer treatment. In this system, AS1411 strands are released from fuel strands as a walker moves along its carbon nanotube track. The release rate and amount of anticancer oligonucleotides are controlled by the walker operation. With a walker system embedded within the collagen extracellular matrix, we show that this drug release system can be used for in situ cancer cell growth inhibition.
In vivo examination of the effects of hydroxycinnamic acid on xenobiotic metabolizing and antioxidant enzymes
SEMİZ, ASLI; Celik-Turgut, Gurbet; KARAKURT, SERDAR; AKÇA, HAKAN; ARSLAN, ŞEVKİ; Adalı, Orhan; ŞEN, ALAATTİN (National Library of Serbia, 2017-01-01)
In the last decade, hydroxycinnamic acids (HCA) have gained increasing attention from researchers due to their antioxidant potential. The aim of this study was to examine in detail the impact of dietary HCA on particular types of P450 and also selected phase II and antioxidant enzymes in Wistar rat. HCA (10 mu M/kg/day, i.p.) was administered for ten continuous days. Examination of the activities and mRNA and protein levels revealed that CYP2B, 2C6 and 3A enzyme activities were not altered significantly, wi...
Citation Formats
T. BAYRAÇ, O. E. Akca, F. I. Eyidogan, and H. A. Öktem, “Target-specific delivery of doxorubicin to human glioblastoma cell line via ssDNA aptamer,” JOURNAL OF BIOSCIENCES, pp. 97–104, 2018, Accessed: 00, 2020. [Online]. Available: