The effects of high cholesterol/high fat diet on endoplasmic reticulum stress and neuronal dysfunction in the hippocampus and cerebral cortex of APOE-/- MICE

Download
2019
Mengi, Naz
Hyperlipidemia is an obesity-associated lipid metabolism disorder with high serum total cholesterol (TC) levels and is known to be a risk factor for neurodegenerative diseases. High-fat diet (HFD) induced elevated inflammation levels accompanied by increased levels of apoptosis markers and decreased levels of synaptic proteins in the hippocampus points out a possible neuronal loss. Protein kinase RNA-like endoplasmic reticulum kinase (PERK) pathway is activated by endoplasmic reticulum (ER) stress. The activation of PERK pathway results in reduced protein synthesis and increased expression of transcription factors involved in the apoptotic and inflammatory response. A possible impact of PERK pathway activation on neuronal failure has been questioned. In this study, the impacts of high cholesterol/high-fat diet (HC/HFD) on the PERK pathway, inflammation, postsynaptic integrity, and neurogenesis were investigated in the hippocampus and the cerebral cortex of ApoE-/- mice by performing Western blot analysis. In the hippocampus, no changes in the PERK pathway was detected, inflammation levels were decreased with Western diet. In the cerebral cortex, the levels of ER stress, inflammation, and postsynaptic integrity proteins were significantly increased in ApoE-/- mice independently of the diet. Neurogenesis was significantly higher in the hippocampus and the cerebral cortex of ApoE-/- mice. Whereas there was no effect of HC/HFD on ER stress in the hippocampus, genotype effect activated the PERK pathway in the cerebral cortex.

Suggestions

Assessment of the impacts of hyperlipidemia on brain and modulation of perk pathway against hyperlipidemia- induced synaptic impairment on hippocampus
Aşkın, Bilge; Yanık, Tülay.; Department of Molecular Biology and Genetics (2019)
Hyperlipidemia is characterized by elevated total cholesterol levels in the circulation, and it is often associated with co-occurrence of neuronal dysfunction and subsequent neurodegeneration. Hyperlipidemia-induced inflammation, synaptic damage and neuronal loss in hippocampus as well as in other regions of the brain consequently leads to cognitive decline. Due to its role in protein translation and cell fate determination, PKR-like endoplasmic reticulum kinase (PERK) arm of the Endoplasmic Reticulum (ER) ...
The effect of diabetes mellitus on rat skeletal extensor digitorum longus muscle tissue: An FTIR study
Bozkurt, Ozlem; Bilgin, Mehmet Dincer; Severcan, Feride (Hindawi Limited, 2007)
Diabetes mellitus (DM) is a chronic disorder of carbohydrate, fat and protein metabolism, which is characterized by a defective insulin secretory response. Skeletal muscle takes role in determination of carbohydrate and lipid metabolism, therefore; it is one of the target tissues of diabetes. Herein this study, application of Fourier Transform Infrared (FTIR) spectroscopy in diabetic skeletal Extensor Digitorum Longus (EDL) muscle tissues will be presented which highlight the promise of this technique in me...
The serum angiotensin converting enzyme and lysozyme levels in patients with ocular involvement of autoimmune and infectious diseases
Sahin, Ozlem; Ziaei, Alireza; Karaismailoğlu, Eda; Taheri, Nusret (Springer Science and Business Media LLC, 2016-2-16)
Background: Increased serum levels of angiotensin converting enzyme and lysozyme are considered as inflammatory markers for diagnosis of sarcoidosis which is an autoimmune inflammatory disease. The purpose of this study is to evaluate the significance of differences in serum angiotensin converting enzyme and lysozyme levels of patients with ocular involvement of other autoimmune inflammatory and infectious diseases. Methods: This is a prospective study involving patients with ankylosing spondylitis, behc...
The association between metabolic syndrome and its components with systemic lupus erythematosus: a comprehensive systematic review and meta-analysis of observational studies
Hallajzadeh, J.; Khoramdad, M.; Izadi, N.; Karamzad, N.; Almasi-Hashiani, A.; Ayubi, E.; Qorbani, M.; Pakzad, R.; Sullman, M. J. M.; Safiri, S. (2018-05-01)
Objectives: Based upon inflammatory-related factors in chronic systemic lupus erythematosus (SLE), as well as the long-term prescription of corticosteroids, metabolic syndrome (MetS) prevalence is expected to be higher in SLE patients than among those without SLE. The aim of this study was to systematically analyze: (1) the worldwide prevalence of MetS in patients with SLE using different criteria, (2) the risk of MetS in patients with SLE compared with those without SLE, and (3) the risk of MetS component ...
Evaluation and discrimination of simvastatin-induced structural alterations in proteins of different rat tissues by FTIR spectroscopy and neural network analysis
Garip, Sebnem; Yapici, Engin; Ozek, Nihal Simsek; Severcan, Mete; Severcan, Feride (Royal Society of Chemistry (RSC), 2010-01-01)
Statins are commonly used to control hypercholesterolemia and to prevent cardiovascular diseases. Among the statins, Simvastatin is one of the most frequently prescribed statins because of its efficacy in reducing LDL lipoprotein cholesterol levels, its tolerability, and its reduction of cardiovascular risk and mortality. Conflicting results have been reported with regard to benefits (pleiotropic effects) as well as risks (adverse effects) of simvastatin on different soft and hard tissues. In the current st...
Citation Formats
N. Mengi, “The effects of high cholesterol/high fat diet on endoplasmic reticulum stress and neuronal dysfunction in the hippocampus and cerebral cortex of APOE-/- MICE,” Thesis (M.S.) -- Graduate School of Natural and Applied Sciences. Molecular Biology and Genetics, Middle East Technical University, 2019.