Addition of Selenium Nanoparticles to Electrospun Silk Scaffold Improves the Mammalian Cell Activity While Reducing Bacterial Growth

Chung, Stanley
Ercan, Batur
Roy, Amit K.
Webster, Thomas J.
Silk possesses many beneficial wound healing properties, and electrospun scaffolds are especially applicable for skin applications, due to their smaller interstices and higher surface areas. However, purified silk promotes microbial growth. Selenium nanoparticles have shown excellent antibacterial properties and are a novel antimicrobial chemistry. Here, electrospun silk scaffolds were doped with selenium nanoparticles to impart antibacterial properties to the silk scaffolds. Results showed significantly improved bacterial inhibition and mild improvement in human dermal fibroblast metabolic activity. These results suggest that the addition of selenium nanoparticles to electrospun silk is a promising approach to improve wound healing with reduced infection, without relying on antibiotics.


Electrospun Silk Doped with Selenium for Antibacterial Skin Applications
Chung, Stanley; Ercan, Batur; Webster, Thomas J.; Phong Tran, Phong Tran (2014-01-01)
Skin is the largest organ by surface area and provides the primary defense against microbes, viruses, and other harmful environmental interactions. Silk is widely used as a cosmetic additive and has been investigated for skin regeneration because of its beneficial properties for skin application : promotion of collagen synthesis, re-epithelialization, wound healing, atopic dermatitis alleviation, and scar reduction. However, pure silk shows negligible or even negative antibacterial properties. Here, we prop...
Sol-gel processing of silver-doped silica and assessment of its structural and antimicrobial properties
Akköprü Akgün, Betül; Durucan, Caner; Department of Metallurgical and Materials Engineering (2015)
Sol-gel processing routes for development of silver biocide-doped antibacterial silicate based coatings and powders were established and structural properties defining the antibacterial activity and long term durability were determined. During the first phase of this study, silver-doped silica based powders have been synthesized by hydrolysis and condensation of tetraethyl orthosilicate (TEOS, Si(OC2H5)4) in the presence of a silver nitrate (AgNO3) solution. The effects of modifications in sol-gel chemistry...
Collagen/gold nanoparticle nanocomposites: A potential skin wound healing biomaterial
Akturk, Omer; Kismet, Kemal; Yasti, Ahmet C.; Kuru, Serdar; Duymus, Mehmet E.; Kaya, Feridun; Caydere, Muzaffer; Hucumenoglu, Sema; Keskin, Dilek (2016-08-01)
In this study, nanocomposite collagen scaffolds incorporating gold nanoparticles (AuNPs) were prepared for wound healing applications. Initially, dose (<20ppm) and size (>20nm) of AuNPs that were not cytotoxic on HaCat keratinocytes and 3T3 fibroblasts were determined. Both collagen sponges and AuNP-incorporated nanocomposites (CS-Au) were cross-linked with glutaraldehyde (CS-X and CS-AuX). Incorporation of AuNPs into cross-linked scaffolds enhanced their stability against enzymatic degradation and increase...
Bostancı, Nazlı Seray; Tezcaner, Ayşen; Hasırcı, Nesrin; Department of Biotechnology (2021-8-10)
The aim of this study was to develop an easily applicable and bioactive skin graft composed of methacrylated pectin (PeMA) and methacrylated gelatin (GelMA), containing curcumin and/or Vitamin-C as antimicrobial and healing accelerating agents, respectively. Grafts were prepared in slab, 3D printed and 3D bioprinted forms and different properties were evaluated. Preparation parameters were optimized using slabs having different compositions (PeMA:GelMA as P:G 1:1, 1:2 and 1:3, v/v). P1:G1 solution did not h...
Mechanism of Photolytic Decomposition of N-Halamine Antimicrobial Siloxane Coatings
Kocer, Hasan B.; Akdağ, Akın; Worley, S. D.; Acevedo, Orlando; Broughton, R. M.; Wu, Yonnie (2010-08-01)
Generally, antimicrobial N halamine siloxane coatings can be rehalogenated repetitively upon loss of their biocidal efficacies, a marked advantage over coatings containing other antimicrobial materials. However, the N halamine materials tend to slowly decompose upon exposure to ultraviolet irradiation as in direct sunlight. In this work the mechanism of photolytic decomposition for the N halamine siloxanes has been studied using spectroscopic and theoretical methods. It was found that the N chlorinated coat...
Citation Formats
S. Chung, B. Ercan, A. K. Roy, and T. J. Webster, “Addition of Selenium Nanoparticles to Electrospun Silk Scaffold Improves the Mammalian Cell Activity While Reducing Bacterial Growth,” FRONTIERS IN PHYSIOLOGY, pp. 0–0, 2016, Accessed: 00, 2020. [Online]. Available: