Addition of Selenium Nanoparticles to Electrospun Silk Scaffold Improves the Mammalian Cell Activity While Reducing Bacterial Growth

Date

2016-07-14

Author

Chung, Stanley
Ercan, Batur
Roy, Amit K.
Webster, Thomas J.

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

156
views

0
downloads

Silk possesses many beneficial wound healing properties, and electrospun scaffolds are especially applicable for skin applications, due to their smaller interstices and higher surface areas. However, purified silk promotes microbial growth. Selenium nanoparticles have shown excellent antibacterial properties and are a novel antimicrobial chemistry. Here, electrospun silk scaffolds were doped with selenium nanoparticles to impart antibacterial properties to the silk scaffolds. Results showed significantly improved bacterial inhibition and mild improvement in human dermal fibroblast metabolic activity. These results suggest that the addition of selenium nanoparticles to electrospun silk is a promising approach to improve wound healing with reduced infection, without relying on antibiotics.

Subject Keywords

Silk, Electrospinning, Antibacterial nanoparticles

URI

https://hdl.handle.net/11511/44332

Journal

FRONTIERS IN PHYSIOLOGY

DOI

https://doi.org/10.3389/fphys.2016.00297

Collections

Department of Metallurgical and Materials Engineering, Article

Suggestions

OpenMETU
Core

Electrospun Silk Doped with Selenium for Antibacterial Skin Applications Chung, Stanley; Ercan, Batur; Webster, Thomas J.; Phong Tran, Phong Tran (2014-01-01) Skin is the largest organ by surface area and provides the primary defense against microbes, viruses, and other harmful environmental interactions. Silk is widely used as a cosmetic additive and has been investigated for skin regeneration because of its beneficial properties for skin application : promotion of collagen synthesis, re-epithelialization, wound healing, atopic dermatitis alleviation, and scar reduction. However, pure silk shows negligible or even negative antibacterial properties. Here, we prop...
Collagen/gold nanoparticle nanocomposites: A potential skin wound healing biomaterial Akturk, Omer; Kismet, Kemal; Yasti, Ahmet C.; Kuru, Serdar; Duymus, Mehmet E.; Kaya, Feridun; Caydere, Muzaffer; Hucumenoglu, Sema; Keskin, Dilek (2016-08-01) In this study, nanocomposite collagen scaffolds incorporating gold nanoparticles (AuNPs) were prepared for wound healing applications. Initially, dose (<20ppm) and size (>20nm) of AuNPs that were not cytotoxic on HaCat keratinocytes and 3T3 fibroblasts were determined. Both collagen sponges and AuNP-incorporated nanocomposites (CS-Au) were cross-linked with glutaraldehyde (CS-X and CS-AuX). Incorporation of AuNPs into cross-linked scaffolds enhanced their stability against enzymatic degradation and increase...
DEVELOPMENT OF 3D PRINTED PECTIN AND GELATIN BASED BIOACTIVE SKIN GRAFTS Bostancı, Nazlı Seray; Tezcaner, Ayşen; Hasırcı, Nesrin; Department of Biotechnology (2021-8-10) The aim of this study was to develop an easily applicable and bioactive skin graft composed of methacrylated pectin (PeMA) and methacrylated gelatin (GelMA), containing curcumin and/or Vitamin-C as antimicrobial and healing accelerating agents, respectively. Grafts were prepared in slab, 3D printed and 3D bioprinted forms and different properties were evaluated. Preparation parameters were optimized using slabs having different compositions (PeMA:GelMA as P:G 1:1, 1:2 and 1:3, v/v). P1:G1 solution did not h...
Mechanism of Photolytic Decomposition of N-Halamine Antimicrobial Siloxane Coatings Kocer, Hasan B.; Akdağ, Akın; Worley, S. D.; Acevedo, Orlando; Broughton, R. M.; Wu, Yonnie (2010-08-01) Generally, antimicrobial N halamine siloxane coatings can be rehalogenated repetitively upon loss of their biocidal efficacies, a marked advantage over coatings containing other antimicrobial materials. However, the N halamine materials tend to slowly decompose upon exposure to ultraviolet irradiation as in direct sunlight. In this work the mechanism of photolytic decomposition for the N halamine siloxanes has been studied using spectroscopic and theoretical methods. It was found that the N chlorinated coat...
Topical delivery of heparin with polymeric nanoparticles on electrospun nanofibers Akolpoğlu, Duygu Deniz; Keskin, Dilek; Department of Biotechnology (2019) Skin surrounds underlying organs and preserve the body against harmful microbial, thermal and mechanical effects and skin damages may give rise to crucial disorders or even death. Therefore, skin regeneration is one of the most important issues in tissue engineering. Search on more effective biomaterials that will enhance regeneration while enabling requirements of a healing skin site is an important issue. In this study, heparin was encapsulated in poly(lactic-co-glycolic acid) (PLGA) nanoparticles (NPs) a...

Citation Formats

S. Chung, B. Ercan, A. K. Roy, and T. J. Webster, “Addition of Selenium Nanoparticles to Electrospun Silk Scaffold Improves the Mammalian Cell Activity While Reducing Bacterial Growth,” FRONTIERS IN PHYSIOLOGY, pp. 0–0, 2016, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/44332.