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Topical delivery of heparin with polymeric nanoparticles on electrospun nanofibers

Akolpoğlu, Duygu Deniz
Skin surrounds underlying organs and preserve the body against harmful microbial, thermal and mechanical effects and skin damages may give rise to crucial disorders or even death. Therefore, skin regeneration is one of the most important issues in tissue engineering. Search on more effective biomaterials that will enhance regeneration while enabling requirements of a healing skin site is an important issue. In this study, heparin was encapsulated in poly(lactic-co-glycolic acid) (PLGA) nanoparticles (NPs) and these synthesized NPs were incorporated to Sericin/Gelatin (Ser/Gel) nanofibers which are obtained with the electrospinning method and evaluated for their potency for Skin Tissue Engineering (STE). Sericin and gelatin were chosen as scaffold material to promote the regeneration of skin with these biopolymers and maximize the water retention capacity of the scaffolds which is important for STE. The aim of the use of HP-loaded NP is to provide rapid and effective healing via prolonged and local release of HP in the wound area. The loading capacity and entrapment efficiency of the nanoparticles were determined as nearly 3% and 60% respectively. Cumulative release of HP from NPs and NP loaded 0/1 and 1/2 Ser/Gel ratio having scaffolds in PBS (pH 7.4, 37°C) was determined as approximately 85%, 60% and 40% over 1-week period, respectively. Cell viability analysis for scaffolds and heparin loaded PLGA nanoparticles on L929 mouse fibroblast cell line was determined as nearly 80% and higher than 100%, respectively. In the scope of these results, the produced NPs and NP loaded scaffolds were ideal for STE applications.