Topical delivery of heparin with polymeric nanoparticles on electrospun nanofibers

Akolpoğlu, Duygu Deniz
Skin surrounds underlying organs and preserve the body against harmful microbial, thermal and mechanical effects and skin damages may give rise to crucial disorders or even death. Therefore, skin regeneration is one of the most important issues in tissue engineering. Search on more effective biomaterials that will enhance regeneration while enabling requirements of a healing skin site is an important issue. In this study, heparin was encapsulated in poly(lactic-co-glycolic acid) (PLGA) nanoparticles (NPs) and these synthesized NPs were incorporated to Sericin/Gelatin (Ser/Gel) nanofibers which are obtained with the electrospinning method and evaluated for their potency for Skin Tissue Engineering (STE). Sericin and gelatin were chosen as scaffold material to promote the regeneration of skin with these biopolymers and maximize the water retention capacity of the scaffolds which is important for STE. The aim of the use of HP-loaded NP is to provide rapid and effective healing via prolonged and local release of HP in the wound area. The loading capacity and entrapment efficiency of the nanoparticles were determined as nearly 3% and 60% respectively. Cumulative release of HP from NPs and NP loaded 0/1 and 1/2 Ser/Gel ratio having scaffolds in PBS (pH 7.4, 37°C) was determined as approximately 85%, 60% and 40% over 1-week period, respectively. Cell viability analysis for scaffolds and heparin loaded PLGA nanoparticles on L929 mouse fibroblast cell line was determined as nearly 80% and higher than 100%, respectively. In the scope of these results, the produced NPs and NP loaded scaffolds were ideal for STE applications.


Nanostructured anti-bacterial poly-lactic-co-glycolic acid films for skin tissue engineering applications
Karahaliloglu, Zeynep; Ercan, Batur; Chung, Stanley; Taylor, Erik; DENKBAŞ, EMİR BAKİ; Webster, Thomas J. (Wiley, 2014-12-01)
Major issues faced with the use of today's skin grafts are infection, scar tissue formation, insufficient keratinocyte (or skin producing cells) proliferation and high production costs. To overcome these limitations, we propose here for the first time, a nanofeatured poly(lactide-co-glycolide) (PLGA) membrane as a next generation antibacterial skin graft material. An alkaline surface treatment method was used to create random nanofeatures on PLGA membranes where sodium hydroxide (NaOH) concentration and exp...
Development and characterization of a tissue engineered multicomponent skin substitute and a skin model
Eke, Gözde; Hasırcı, Vasıf Nejat; Hasırcı, Nesrin; Department of Biotechnology (2018)
The tissue engineered human skin substitute has the potential to fill large areas of skin loss caused by severe burns or chronic wounds. It can also serve as an alternative skin model to in vivo testing of drugs or cosmetic products. The aim was to construct a tissue engineered full-thickness human skin model mimicking the native tissue. To this end, this model was developed as epidermis, dermis and subcutaneous tissue of the skin. The feasibility of the dermis layer was tested by co-culturing fibroblasts a...
Topical delivery of heparin from PLGA nanoparticles entrapped in nanofibers of sericin/gelatin scaffolds for wound healing
Basaran, Duygu Deniz Akolpoglu; Gündüz, Ufuk; Tezcaner, Ayşen; Keskin, Dilek (2021-03-01)
Skin regeneration is one of the most important issues in tissue engineering. Research on more effective biomaterials that will enhance regeneration while enabling requirements of a healing skin site is an important challenge in skin tissue engineering. In this study, heparin was encapsulated in Poly(lactic-co-glycolic acid) (PLGA) nanoparticles (NPs) which were then incorporated into Sericin/Gelatin (Ser/Gel) nanofibers during the electrospinning process in order to develop a combined system that has contro...
Biodegradable poly (ester-urethane) scaffolds for bone tissue engineering
Kızıltay, Aysel; Hasırcı, Nesrin; Department of Biotechnology (2011)
During last decade, polyurethanes (PUs) which are able to degrade into harmless molecules upon implantation have received a significant level of attention as a biomaterial in tissue engineering applications. Many studies are focused especially on development of PUs based on amino acid derivatives; however, there are only few applications of amino acid based PUs in tissue engineering. In this study, a biocompatible and biodegradable thermoplastic poly(ester-urethane) (PEU) based on L-lysine diisocyanate (LDI...
Addition of Selenium Nanoparticles to Electrospun Silk Scaffold Improves the Mammalian Cell Activity While Reducing Bacterial Growth
Chung, Stanley; Ercan, Batur; Roy, Amit K.; Webster, Thomas J. (2016-07-14)
Silk possesses many beneficial wound healing properties, and electrospun scaffolds are especially applicable for skin applications, due to their smaller interstices and higher surface areas. However, purified silk promotes microbial growth. Selenium nanoparticles have shown excellent antibacterial properties and are a novel antimicrobial chemistry. Here, electrospun silk scaffolds were doped with selenium nanoparticles to impart antibacterial properties to the silk scaffolds. Results showed significantly im...
Citation Formats
D. D. Akolpoğlu, “Topical delivery of heparin with polymeric nanoparticles on electrospun nanofibers,” Thesis (M.S.) -- Graduate School of Natural and Applied Sciences. Biotechnology., Middle East Technical University, 2019.