Development and characterization of (carbonated) hydroxyapatite, akermanite involving polycaprolactone scaffolds

Download

index.pdf

Date

2019

Author

Altunordu, Gerçem

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

267
views

123
downloads

Bone tissue loss or damage can occur due to accidents, injuries and diseases (osteomyelitis, osteosarcaoma, osteoporosis, etc.). Bone tissue engineering (BTE) aims to develop biomaterials that enhance the regeneration of bone tissue. Therefore, scaffolds for BTE should be biocompatible and bioactive; enabling strong communication with bone tissue. Bioceramics are a group of biomaterials that resemble to native bone mineral and improve bioactivity of BTE scaffolds. Considering these hydroxyapatite (HA), carbonated hydroxyapatite (CHA) and akermanite (AKR) bioceramics were synthesized and their optimum combination was determined to be used as BTE scaffolds. Bioactivities of all bioceramics were good. However, it was seen that AKR makes the ambient pH of the incubation medium basic. Therefore, 90% HA-10% AKR and 90% CHA-10% AKR bioceramic groups were prepared and compared with only HA or CHA containing groups. Bioceramic-polymer composite scaffolds were prepared by wet electrospinning of poly ԑ-caprolactone (PCL) solution loaded with these bioceramics (10% w/w) and then characterized for degradation, swelling and microstructural properties. Scaffolds containing CHA and AKR were shown to highly support apatite formation. Additionaly, cell proliferation and osteogenic activity were studied with bone cells (Saos-2). Among all, PCL scaffolds containing 90%HA-10%AKR and 90%CHA-10%AKR minerals supported better cell proliferation. It was observed that all groups induced cell differentiation with apatite layer formation. Therefore, all scaffolds groups are considered to be promising for use in bone tissue engineering applications.

Subject Keywords

Akermanite., Hydroxyapatite, Akermanite, Bone Tissue Engineering, Electrospinning, PCL.

URI

http://etd.lib.metu.edu.tr/upload/12624629/index.pdf
https://hdl.handle.net/11511/44542

Collections

Graduate School of Natural and Applied Sciences, Thesis

Suggestions

OpenMETU
Core

Improvement of bioactivity with dual bioceramic incorporation to nanofibrous PCL scaffolds Altunordu, Gercem; Tezcaner, Ayşen; Evis, Zafer; Keskin, Dilek (2023-03-01) Bone tissue injuries, diseases or related clinical interventions require bone tissue engineering (BTE) approaches for regeneration of large bone defects, especially for compromised situations. Most BTE applications in literature focused on composites of polymers with a single type of bioceramic. However, native bone matrix has various inorganic components. Accordingly, this study aimed to investigate the use of dual bioceramics in BTE scaffolds prepared by wet-electrospinning of Poly-caprolactone (PCL) and ...
Design and Development of a Low Cost Device for Bone Fracture Detection Using FFT Technique on MATLAB Qadir, Zakria; Ali, Muhammad; Nesimoglu, Tayfun (2018-11-02) Bone fracture is a common problem in daily life which occurs when high pressure is applied on bone or by simple accident and also due to osteoporosis and bone cancer. Different techniques are used today to detect bone fractures such as X-Ray, Computed Tomography (CT-scan), Magnetic Resonance Imaging (MRI) and Ultrasound. Among these four modalities, X-ray diagnosis is commonly used for fracture detection. However, if the fracture is complicated, a CT scan or MRI may be needed for further diagnosis and opera...
Development of a siRNA delivery system for the treatment of osteoporosis Sezlev Bilecen, Deniz; Hasırcı, Vasıf Nejat; Uludağ, Hasan; Department of Biotechnology (2018) Osteoporosis, the most common disease of bone, is a skeletal disorder associated with low bone mass, increase in bone fragility and in susceptibility to fractures. The high bone resorption rate is shown to be due to increased number and activity of the osteoclasts. Receptor Activator of Nuclear Factor kappa B (RANK)/ Receptor Activator of Nuclear Factor kappa B Ligand (RANKL) system plays a crucial role in osteoclast differentiation and bone remodeling. RANKL participates in differentiation and activation o...
Mesenchymal Stem Cells and Nano-Bioceramics for Bone Regeneration Kankilic, Berna; Kose, Sevil; KORKUSUZ, PETEK; Timucin, Muharrem; Korkusuz, Feza (2016-01-01) Orthopedic disorders and trauma usually result in bone loss. Bone grafts are widely used to replace this tissue. Bone grafts excluding autografts unfortunately have disadvantages like evoking immune response, contamination and rejection. Autografts are of limited sources and optimum biomaterials that can replace bone have been searched for several decades. Bioceramics, which have the similar inorganic structure of natural bone, are widely used to regenerate bone or coat metallic implants. As people continuo...
Local delivery of bioactive agents for bone tissue engineering Alissa Alam, Hani; Keskin, Dilek; Özen, Can; Department of Biotechnology (2017) Many cases of bone defects require bone grafts addition into the body. Bone tissue substitutes have more advantages over other bone grafts because of low infection risk and abundance of materials. Local delivery of drugs or bioactive agents can help in these diseases’ treatments and bone growth overall while avoiding side effects or drug interference. To accelerate local bone regrowth, a research about developing a controlled local release system of bioactive agents to bone cells was conducted in this study...

Citation Formats

G. Altunordu, “Development and characterization of (carbonated) hydroxyapatite, akermanite involving polycaprolactone scaffolds,” Thesis (M.S.) -- Graduate School of Natural and Applied Sciences. Biomedical Engineering., Middle East Technical University, 2019.