DIFFERENTIAL GENE EXPRESSION OF PNCES A4 IN POPULUS NIGRA

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Raitha Akkad -MSc thesis.pdf

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2022-5-27

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Akkad, Raitha

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Populus nigra L. owes to be the most critical pioneer tree species in riparian ecosystems and is considered an ecologically and economically-wise imperative fast-growing species. Moreover, it is a great tree species for biomass and bioenergy utilization and cellulose content manipulation. The limited energy resources from fossil fuels demand an alternative efficient biomass crop, where Populus nigra shows to be a great candidate. In this study, the CesA (cellulose synthase) secondary cell wall-related CesA4 subunit gene expression (PnCESA4) and its relation to cellulose were investigated in Populus nigra L. The cellulose synthase complex (CSC) involving the CESA subunits, PnCesA4/ 7/ and 8, for secondary cell wall regulation and involvement, was not yet fully discovered. Thus, from the CesA secondary cell wall-related subunit genes the expression of the PnCesA4 subunit gene, which is the only single-copy cellulose synthase gene, was investigated in Populus nigra trees. In addition to PnCesA4 gene expression, its relation to UGPase and SuSy activity, and growth data which were provided by Taşkıran (2020) were explored to understand the relationship among cellulose-related traits. The results of the study surprisingly are in contrary to what was expected that is, there were negative correlations between PnCesA4 gene expression levels and growth traits (Height and Diameter). Moreover, interestingly PnCesA4 gene expression was not correlated with cellulose content or cellulose synthesizing enzymes. These outcomes taken altogether, indicate in owing to a more complicated regulation in the pathway that is taking place, and a more unique functional role for the PnCesA4 subunit that is yet to be known.

Subject Keywords

Cellulose synthase, PnCesA4, Populus nigra, Cellulose, and Gene Expression

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https://hdl.handle.net/11511/97938

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Graduate School of Natural and Applied Sciences, Thesis

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Citation Formats

R. Akkad, “DIFFERENTIAL GENE EXPRESSION OF PNCES A4 IN POPULUS NIGRA,” M.S. - Master of Science, Middle East Technical University, 2022.