Akkad, Raitha
Populus nigra L. owes to be the most critical pioneer tree species in riparian ecosystems and is considered an ecologically and economically-wise imperative fast-growing species. Moreover, it is a great tree species for biomass and bioenergy utilization and cellulose content manipulation. The limited energy resources from fossil fuels demand an alternative efficient biomass crop, where Populus nigra shows to be a great candidate. In this study, the CesA (cellulose synthase) secondary cell wall-related CesA4 subunit gene expression (PnCESA4) and its relation to cellulose were investigated in Populus nigra L. The cellulose synthase complex (CSC) involving the CESA subunits, PnCesA4/ 7/ and 8, for secondary cell wall regulation and involvement, was not yet fully discovered. Thus, from the CesA secondary cell wall-related subunit genes the expression of the PnCesA4 subunit gene, which is the only single-copy cellulose synthase gene, was investigated in Populus nigra trees. In addition to PnCesA4 gene expression, its relation to UGPase and SuSy activity, and growth data which were provided by Taşkıran (2020) were explored to understand the relationship among cellulose-related traits. The results of the study surprisingly are in contrary to what was expected that is, there were negative correlations between PnCesA4 gene expression levels and growth traits (Height and Diameter). Moreover, interestingly PnCesA4 gene expression was not correlated with cellulose content or cellulose synthesizing enzymes. These outcomes taken altogether, indicate in owing to a more complicated regulation in the pathway that is taking place, and a more unique functional role for the PnCesA4 subunit that is yet to be known.


Genetic differentiation of European black poplar (Populus Nigra L.) clones and populations with respect to some enzymes involved in biosynthesis of cellulose and lignin
Taşkıran, Bircan; Kaya, Zeki; Department of Biology (2020)
The European black poplar, (Populus nigra L.) is ecologically and economically important fast-growing trees. Its wide distribution range in northern hemisphere, easy vegetative propagation, rapid growth rate and hybridization ability, and also its biomass source potential make European black poplar a perfect candidate for lignocellulosic biomass production. In this study, specific activities of two important cellulose related (SuSy and UGPase) and three lignin pathway related (PAL, 4CL, and CAD) enzymes, we...
Differential expression of cold resistant related genes in populus nigra l. clones
Zeybek, Evrim; Kaya, Zeki; Department of Biology (2014)
Poplar has been adopted as a model perennial woody species for forest tree genetics to understand molecular processes of growth, development and responses to environmental stresses. In this study, seasonal changes in antioxidant enzymes activities including glutathione reductase (GR), ascorbate peroxidase (APX), dehydroascorbate reductase (DHAR) and catalase (CAT) were examined in black poplar (Populus nigra L.) clones. It was found that GR, APX and DHAR activities increased with low air temperatures in win...
Durgut, Bilge; Kaya, Zeki; Department of Molecular Biology and Genetics (2022-2-23)
Salix alba and Populus nigra are two important tree species of riparian ecosystems. With a great ecologic and economic significance, they are naturally distributed in almost all river basins of Turkey. The genetic structures of these species in two river basins in Turkey were studied using eight common microsatellite markers to both species to reveal the impacts of habitat fragmentation. Therefore, the studied rivers were selected to represent a highly fragmented river, and a protected river from habitat fr...
Genetic differentiation between clone collections and natural populations of European black poplar (Populus nigra L.) in turkey
Çiftçi, Asiye; Kucukosmanoglu, Filiz; Karahan, Alptekin; Kaya, Zeki (2017-06-01)
The European black poplar (Populus nigra L.) is an ecologically and economically important tree species for Turkey. The important and major genetic resources of species for future breeding and ex situ conservation purposes have been archived in a clone bank in Ankara by selecting clones from natural populations and old plantations throughout Turkey. There is no study to date assessing genetic composition these materials. Two-hundred-thirty-three P. nigra clones from six geographic region of Turkey (clone co...
Genetic control of cellulose, lignin and glucose contents in european black poplar (populus nigra l.) populations from Turkey
Taşkıran, Bircan; Kaya, Zeki; Severcan, Feride; Department of Biology (2014)
Populus nigra L. is considered as one of the most economically significant forest tree species with respect to production of wood, biomass, timber, pulp, paper and other wood-based products, besides its ecological and evolutionary importance. Because of the increased wood needs of the world and demands of renewable energy sources, fast-growing poplar has gained importance. While wood quality, pulp mechanical strength, and biomass are directly associated with high cellulose content, lignin emerges as an unde...
Citation Formats
R. Akkad, “DIFFERENTIAL GENE EXPRESSION OF PNCES A4 IN POPULUS NIGRA,” M.S. - Master of Science, Middle East Technical University, 2022.