Topical delivery of heparin from PLGA nanoparticles entrapped in nanofibers of sericin/gelatin scaffolds for wound healing

Date

2021-03-01

Author

Basaran, Duygu Deniz Akolpoglu
Gündüz, Ufuk
Tezcaner, Ayşen
Keskin, Dilek

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

130
views

0
downloads

Skin regeneration is one of the most important issues in tissue engineering. Research on more effective biomaterials that will enhance regeneration while enabling requirements of a healing skin site is an important challenge in skin tissue engineering. In this study, heparin was encapsulated in Poly(lactic-co-glycolic acid) (PLGA) nanoparticles (NPs) which were then incorporated into Sericin/Gelatin (Ser/Gel) nanofibers during the electrospinning process in order to develop a combined system that has controlled release approach, besides the ability to help the regeneration of skin tissue by the involvement of biopolymers; gelatin, and sericin. The loading capacity and heparin encapsulation efficiency in the nanoparticles were determined as 30.04 mg/g of polymer and 60%, respectively. Cumulative release of heparin from NPs for 1 week was faster than from NPs loaded gelatin scaffolds and from dual protein (Ser/Gel) scaffolds with ratios: 1/7 and 1/2 (approximately 85%, 65%, 55%, and 40%, respectively). Sericin addition slowed down the degradation properties of the scaffold. The scaffold having a Ser/Gel ratio (1/2) was found as the most promising candidate because of its proper fiber morphology, high water retention, and low degradation degree.

URI

https://hdl.handle.net/11511/89758

Journal

INTERNATIONAL JOURNAL OF PHARMACEUTICS

DOI

https://doi.org/10.1016/j.ijpharm.2021.120207

Collections

Graduate School of Natural and Applied Sciences, Article

Suggestions

OpenMETU
Core

Topical delivery of heparin with polymeric nanoparticles on electrospun nanofibers Akolpoğlu, Duygu Deniz; Keskin, Dilek; Department of Biotechnology (2019) Skin surrounds underlying organs and preserve the body against harmful microbial, thermal and mechanical effects and skin damages may give rise to crucial disorders or even death. Therefore, skin regeneration is one of the most important issues in tissue engineering. Search on more effective biomaterials that will enhance regeneration while enabling requirements of a healing skin site is an important issue. In this study, heparin was encapsulated in poly(lactic-co-glycolic acid) (PLGA) nanoparticles (NPs) a...
Native extracellular matrix/fibroin hydrogels for adipose tissue engineering with enhanced vascularization Kayabolen, Alisan; Keskin, Dilek; Aykan, Andac; Karslioglu, Yildirim; Zor, Fatih; Tezcaner, Ayşen (IOP Publishing, 2017-06-23) Adipose tissue engineering is a promising field for regeneration of soft tissue defects. However, vascularization is needed since nutrients and oxygen cannot reach cells in thick implants by diffusion. Obtaining a biocompatible scaffold with good mechanical properties is another problem. In this study, we aimed to develop thick and vascularized adipose tissue constructs supporting cell viability and adipose tissue regeneration. Hydrogels were prepared by mixing rat decellularized adipose tissue (DAT) and si...
Metabolomics analysis of iPSCs derived organoids Sarı , Vedat; Koçak , Gamze; Reçber, Tuba; Karakülah , Gökhan; Utine , Canan Aslı; Nemutlu , Emirhan; Güven, Sinan (Orta Doğu Teknik Üniversitesi Enformatik Enstitüsü; 2022-10) Regenerative medicine is focused on functional restoration of a particular tissue and organs. Stem cell based miniaturized heterogenous cellular clusters named organoids, exhibit features of organ functionality, self-organization, multicellularity, and utility. Induced pluripotent stem cells (iPSCs), have great potential to develop organoids of specific tissues and utilized in regenerative medicine. Organoid technologies have revolutionized the approach to human development and diseases, providing outstandi...
Wet spun PCL scaffolds for tissue engineering Malikmammadov, Elbay; Hasırcı, Nesrin; Endoğan Tanır, Tuğba; Department of Micro and Nanotechnology (2017) Scaffolds produced for tissue engineering applications are promising alternatives to be used in healing and regeneration of injured tissues and organs. In this study, fibrous poly(ε-caprolactone) (PCL) scaffolds were prepared by wet spinning technique and modified by addition of β-tricalcium phosphate (β-TCP) and by immobilizing gelatin onto fibers. Meanwhile, gelatin microspheres carrying Ceftriaxone sodium (CS), a model antibiotic, were added onto the scaffolds and antimicrobial activity of CS was investi...
Nanostructured anti-bacterial poly-lactic-co-glycolic acid films for skin tissue engineering applications Karahaliloglu, Zeynep; Ercan, Batur; Chung, Stanley; Taylor, Erik; DENKBAŞ, EMİR BAKİ; Webster, Thomas J. (Wiley, 2014-12-01) Major issues faced with the use of today's skin grafts are infection, scar tissue formation, insufficient keratinocyte (or skin producing cells) proliferation and high production costs. To overcome these limitations, we propose here for the first time, a nanofeatured poly(lactide-co-glycolide) (PLGA) membrane as a next generation antibacterial skin graft material. An alkaline surface treatment method was used to create random nanofeatures on PLGA membranes where sodium hydroxide (NaOH) concentration and exp...

Citation Formats

D. D. A. Basaran, U. Gündüz, A. Tezcaner, and D. Keskin, “Topical delivery of heparin from PLGA nanoparticles entrapped in nanofibers of sericin/gelatin scaffolds for wound healing,” INTERNATIONAL JOURNAL OF PHARMACEUTICS, pp. 0–0, 2021, Accessed: 00, 2021. [Online]. Available: https://hdl.handle.net/11511/89758.