Global Regulatory Systems Operating in Bacilysin Biosynthesis in Bacillus subtilis

Date

2011-01-01

Author

Koroglu, Turkan Ebru
Ogulur, Ismail
Mutlu, Seval
Karataş, Ayten
Özcengiz, Gülay

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In Bacillus subtilis, bacilysin is a nonribosomally synthesized dipeptide antibiotic composed of L-alanine and L-anticapsin. The biosynthesis of bacilysin depends on the bacABCDEywfG operon (bac operon) and the adjacent ywfH gene. To elucidate the effects of global regulatory genes on the expression of bac operon, we used the combination of lacZ fusion analysis and the gel mobility shift assays. The cell density-dependent transition state induction of the bac operon was clearly shown. The basal expression level of the bac operon as well as transition state induction of bac is directly ComA dependent. Three Phr peptides, PhrC, PhrF and PhrK, are required for full-level expression of ComA-dependent bac operon expression, but the most important role seemed to be played by PhrC in stimulating bac expression through a RapC-independent manner. Spo0A is another positive regulator which participates in the transition state induction of bac both directly by interacting with the bac promoter and indirectly by repressing abrB expression. AbrB and CodY proteins do not only directly repress the bac promoter, but they also mutually stimulate the transition state induction of bac indirectly, most likely by antagonizing their repressive effects without preventing each other's binding since both proteins can bind to the bac promoter simultaneously. Copyright (C) 2011 S. Karger AG, Basel

Subject Keywords

Biotechnology, Applied Microbiology and Biotechnology, Molecular Biology, Microbiology

URI

https://hdl.handle.net/11511/47580

Journal

JOURNAL OF MOLECULAR MICROBIOLOGY AND BIOTECHNOLOGY

DOI

https://doi.org/10.1159/000328639

Collections

Department of Biology, Article

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Citation Formats

T. E. Koroglu, I. Ogulur, S. Mutlu, A. Karataş, and G. Özcengiz, “Global Regulatory Systems Operating in Bacilysin Biosynthesis in Bacillus subtilis,” JOURNAL OF MOLECULAR MICROBIOLOGY AND BIOTECHNOLOGY, pp. 144–155, 2011, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/47580.