Screening and In Situ Monitoring of Potential Petroleum Hydrocarbon Degraders in Contaminated Surface Water

Incomplete combustion of fossil fuels and other anthropogenic activities result in contamination of surface water by petroleum hydrocarbons. These pollutants can have severe effects on aquatic life and human health. In petroleum bioremediation, oil degrading microorganisms are utilized to remove petroleum hydrocarbons from polluted water. However, monitoring and identifying microorganisms capable of degrading petroleum hydrocarbons is very challenging. In the current study, bacteria isolated from a river alongside a petroleum refinery were assessed for their petroleum hydrocarbon degradation abilities. Primary selection was performed through traditional enrichment culture method, emulsifying ability measurement, and gravimetric analysis of hydrocarbon degradation. Screening of two novel hydrocarbon catabolism genes encoding key enzymes in hydrocarbon degradation, alkB and C23O, was performed by using polymerase chain reaction. The bacterial isolates, degrading hydrocarbons with high emulsification abilities and harboring the catabolic genes, were identified as species of Acinetobacter by 16S rRNA sequence analysis. Monitoring the temporal distribution of Acinetobacter in river water was also done by fluorescence in situ hybridization using DNA probe ACA652. The results revealed that species of Acinetobacter successfully populate the polluted surface water and have high potential for petroleum bioremediation.


Bioelectricity Generation From Wastewater Sludge Using Microbial Fuel Cells: A Critical Review
Ömeroğlu, Seçil; Sanin, Faika Dilek (Wiley, 2016-09-01)
Today, the majority of the world's energy is provided by fossil fuels. Natural energy resources soon will be consumed as a result of rising energy needs of the growing population, leading to a global energy crisis. The insecurity in the energy market also affects the global economy negatively and forces the governments to investigate renewable energy alternatives such as bioenergy. Bioenergy technologies can reduce greenhouse gas (GHG) emissions significantly and can serve to satisfy many forms of energy de...
Thiamine pyrophosphate dependent enzyme catalyzed reactions: Stereoselective C-Cbond formations in water
Demir, Ayhan Sıtkı; Ayhan, Peruze; Sopaci, S. Betuel (Wiley, 2007-11-01)
Enzymes are biodegradable and renewable resources that utilize water as the reaction solvent. Reactions catalyzed by enzymes are among the most effective, selective, and 'green' processes available today. The advantages of using biocatalysts include their high degree of regio- and stereo-specificity, versatility, and high reaction rates under mild reaction conditions. Enzyme catalyzed enantioselective C-C bond formation reactions are rather important aspects of synthetic organic chemistry. Thiamine pyrophos...
Anaerobic co-digestion of sewage sludge and primary clarifier skimmings for increased biogas production
Alanya, S.; Yılmazel Tokel, Yasemin Dilşad; Park, C.; Willis, J. L.; Keaney, J.; Kohl, P. M.; Hunt, J. A.; Duran, M. (IWA Publishing, 2013-01-01)
The objective of the study was to identify the impact of co-digesting clarifier skimmings on the overall methane generation from the treatment plant and additional energy value of the increased methane production. Biogas production from co-digesting clarifier skimmings and sewage sludge in pilot-scale fed-batch mesophilic anaerobic digesters has been evaluated. The digester was fed with increasing quantities of clarifier skimmings loads: 1.5, 2.6, 3.5 and 7.0 g COD equivalent/(L.d) (COD: chemical oxygen dem...
Performance of ozone and peroxone on the removal of endocrine disrupting chemicals (EDCs) coupled with cost analysis
Ölmez Hancı, Tuğba; Dogruel, S.; Emek, A. D. Allar; Yilmazer, C. Eropak; Cinar, S.; Kiraz, O.; Citil, E.; Orhon, A. Koc; Siltu, E.; Gucver, S. M.; Ozgun, O. Karahan; Tanık, Ayşe Gül; Yetiş, Ülkü (IWA Publishing, 2020-08-01)
Micropollutants such as endocrine disruptors are one of the most important groups of chemicals polluting water resources. Conventional treatment systems may not be effective for the removal of endocrine disrupting chemicals (EDCs), and the fate of these chemicals should be carefully monitored in the effluent of wastewater treatment plants (WWTPs). Additional treatment methods such as advanced oxidation processes can be used for the removal of endocrine disruptors. This study presents the existence of endocr...
Enhancing the performance of anaerobic digestion of dairy manure through phase-separation
Yilmaz, Vedat; Demirer, Göksel Niyazi (Wiley, 2008-09-01)
Anaerobic digestion (AD) is an effective way to convert animal manures into profitable by-products while simultaneously reducing, the pollution of water, air, and soil caused by these wastes. Conventional high-rate anaerobic reactors cannot effectively process animal manures with high solids-containing wastes. The two-phase configuration for AD has several advantages over conventional one-phase processes, e.g., increased stability of the process, smaller size and cost efficient process configurations. In th...
Citation Formats
B. İçgen, “Screening and In Situ Monitoring of Potential Petroleum Hydrocarbon Degraders in Contaminated Surface Water,” CLEAN-SOIL AIR WATER, pp. 0–0, 2017, Accessed: 00, 2020. [Online]. Available: