Reduced adhesion of macrophages on anodized titanium with select nanotube surface features

Rajyalakshmi, Amancherla
Ercan, Batur
Balasubramanian, K.
Webster, Thomas J.
One of the important prerequisites for a successful orthopedic implant apart from being osteoconductive is the elicitation of a favorable immune response that does not lead to the rejection of the implant by the host tissue. Anodization is one of the simplest surface modification processes used to create nanotextured and nanotubular features on metal oxides which has been shown to improve bone formation. Anodization of titanium (Ti) leads to the formation of TiO(2) nanotubes on the surface, and the presence of these nanotubes mimics the natural nanoscale features of bone, which in turn contributes to improved bone cell attachment, migration, and proliferation. However, inflammatory cell responses on anodized Ti remains to be tested. It is hypothesized that surface roughness and surface feature size on anodized Ti can be carefully manipulated to control immune cell (specifically, macrophages) responses. Here, when Ti samples were anodized at 10 V in the presence of 1% hydrofluoric acid (HF) for 1 minute, nanotextured (nonnanotube) surfaces were created. When anodization of Ti samples was carried out with 1% HF for 10 minutes at 15 V, nanotubes with 40-50 nm diameters were formed, whereas at 20 V with 1% HF for 10 minutes, nanotubes with 60-70 nm diameters were formed. In this study, a reduced density of macrophages was observed after 24 hours of culture on nanotextured and nanotubular Ti samples which were anodized at 10, 15, and 20 V, compared with conventional unmodified Ti samples. This in vitro study thus demonstrated a reduced density of macrophages on anodized Ti, thereby providing further evidence of the greater efficacy of anodized Ti for orthopedic applications.


Decreased Staphylococcus aureus biofilm growth on anodized nanotubular titanium and the effect of electrical stimulation
Ercan, Batur; Tarquinio, Keiko M.; Webster, Thomas J. (2011-07-01)
Infection is a major problem in orthopedics leading to implant failure. It is a challenging task to treat orthopedic implant infection, which may lead to implant replacement and, in severe cases, may result in amputation and mortality. Infection poses an even further risk as bacteria are beginning to develop resistance against commonly used antibiotics. Therefore, in this research a combination of various approaches was used to fight implant infection without resorting to the use of antibiotics. Specificall...
Chemical and topographical modification of PHBV surface to promote osteoblast alignment and confinement
Kenar, H.; Kocabas, A.; AYDINLI, ATİLLA; Hasırcı, Vasıf Nejat (2008-06-15)
Proper cell attachment and distribution, and thus stronger association in vivo between a bone implant and native tissue will improve the success of the implant. In this study, the aim was to achieve promotion of attachment and uniform distribution of rat mesenchymal stem cell-derived osteoblasts by introducing chemical and topographical cues on poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) film surfaces. As the chemical cues, either alkaline phosphatase was covalently immobilized on the film surface t...
Enhanced immunostimulatory activity of cyclic dinucleotides on mouse cells when complexed with a cell-penetrating peptide or combined with CpG
Yildiz, Soner; Alpdundar, Esin; Gungor, Bilgi; Kahraman, Tamer; Bayyurt, Banu; GÜRSEL, İHSAN; Gürsel, Mayda (2015-04-01)
Recognition of pathogen-derived nucleic acids by immune cells is critical for the activation of protective innate immune responses. Bacterial cyclic dinucleotides (CDNs) are small nucleic acids that are directly recognized by the cytosolic DNA sensor STING (stimulator of IFN genes), initiating a response characterized by proinflammatory cytokine and type I IFN production. Strategies to improve the immune stimulatory activities of CDNs can further their potential for clinical development. Here, we demonstrat...
Anterior mitral leaflet curvature in the beating ovine heart: a case study using videofluoroscopic markers and subdivision surfaces
Göktepe, Serdar; Kvitting, JPE; Swanson, Julia; Ingels, Neil; Miller, Craig; Kuhl, Ellen (Springer Science and Business Media LLC, 2010-06-01)
The implantation of annuloplasty rings is a common surgical treatment targeted to re-establish mitral valve competence in patients with mitral regurgitation. It is hypothesized that annuloplasty ring implantation influences leaflet curvature, which in turn may considerably impair repair durability. This research is driven by the vision to design repair devices that optimize leaflet curvature to reduce valvular stress. In pursuit of this goal, the objective of this manuscript is to quantify leaflet curvature...
Effects of human granulocyte-colony stimulating factor on fracture healing in rats
Bozlar, M; Aslan, B; Kalaci, A; Baktiroglu, L; Yanat, AN; Tasci, A (2005-08-01)
Objective: Granulocyte colony stimulation factor (G-CSF) is generally used to prevent and cure the neutropenia associated with chemotherapy and bone marrow transplantation. In addition to its effects on neutrophil function, G-CSF was found to have the characteristic of modulating the cytokines in the inflammatory response. Then, the question to answer is whether it has any effect on fracture healing and to what extent? In this study, we test the effects of G-CSF on the healing of tibia fracture in a rat model.
Citation Formats
A. Rajyalakshmi, B. Ercan, K. Balasubramanian, and T. J. Webster, “Reduced adhesion of macrophages on anodized titanium with select nanotube surface features,” INTERNATIONAL JOURNAL OF NANOMEDICINE, pp. 1765–1771, 2011, Accessed: 00, 2020. [Online]. Available: