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BACILYSIN PRODUCTION BY BACILLUS-SUBTILIS - EFFECTS OF BACILYSIN, PH AND TEMPERATURE
Date
1991-01-01
Author
Özcengiz, Gülay
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The dipeptide antibiotic bacilysin, when added externally to the early exponential-phase cultures, markedly limited its own synthesis. It was shown in cell-free extracts that the feedback effect does not involve the inhibition of bacilysin synthetase, the enzyme catalyzing bacilysin formation. We also studied pH and temperature dependence of bacilysin production. Production was highest at about pH 6.8 and at 25-degrees-C.
Subject Keywords
Microbiology
,
General Medicine
URI
https://hdl.handle.net/11511/48704
Journal
FOLIA MICROBIOLOGICA
DOI
https://doi.org/10.1007/bf02884030
Collections
Department of Biology, Article
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Bacilysin, as the simplest peptide antibiotic made up of only L-alanine and L-anticapsin, is produced and excreted by Bacillus subtilis under the control of quorum sensing. We analyzed bacilysin-nonproducing strain OGU1 which was obtained by bacA-targeted pMutin T3 insertion into the parental strain genome resulting in a genomic organization (bacA '::lacZ::erm::bacABCDEF) to form an IPTG-inducible bac operon. Although IPTG induction provided 3- to 5-fold increment in the transcription of bac operon genes, n...
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Bacilysin being produced by Bacillus subtilis is the smallest peptide antibiotic ever known. It is composed of an N-terminal l-alanine and a modified amino acid at its C-terminal, namely anticapsin. bacABCDEF operon and a monocistronic gene bacG are functional for bacilysin production in the organism, bacABCDFG being needed for the flux from prephenate to anticapsin and then to mature bacilysin while bacE gene within the operon is involved in resistance of the producer by pumping bacilysin out of the cell. ...
Bioproduction, structure elucidation and in vitro antiproliferative effect of eumelanin pigment from Streptomyces parvus BSB49
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G. Özcengiz, “BACILYSIN PRODUCTION BY BACILLUS-SUBTILIS - EFFECTS OF BACILYSIN, PH AND TEMPERATURE,”
FOLIA MICROBIOLOGICA
, pp. 522–526, 1991, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/48704.