Isolation and molecular characterization of Salmonella enterica and Escherichia coli from poultry samples

Cesur, Aylin
Ulutas, Sacide Ozlem
Soyer, Yeşim
Innumerable foodborne pathogens including Salmonella and Escherichia coli pose a serious threat to human health and food safety. As perishable foods, poultry products are considered one of the most common sources of foodborne pathogens including Salmonella and E. coli due to transmission of drug resistance, dissemination of organisms, and cross-contamination. In our study, phenotypic and genotypic characterizations of Salmonella enterica and E. coli isolated from packaged raw chicken products were carried out. Samples belonging to different commercial brands were collected in Ankara in 2015. Among 15 out of 19 Salmonella enterica subsp. enterica strains isolated from different and/or same poultry samples were found as Infantis serotype, while 4 of them were identified as Enteritidis serotype by pulsed-field gel electrophoresis (PFGE) footprints. In addition, 19 out of 40 samples gave positive results for E. coli. In addition, PFGE types of Salmonella Infantis isolates were detected as PT 08, 45, and 50. Furthermore, multilocus sequence typing types of the samples were identified as ST 32. Results of the phenotypic and genotypic antimicrobial resistance profiles of Salmonella Infantis and E. coli isolates revealed considerable resistance to nalidixic acid, tetracycline, streptomycin, sulfisoxazole and trimethoprim/sulfamethoxazole. On the other hand, 3 E. coli isolates showed antibiotic susceptibility. All in all, this study might enlighten some molecular features of Salmonella and E. coli isolated from chicken products in Turkey.


Fecal shedding of, antimicrobial resistance in, and serologic response to SalmonellaTyphimurium in dairy calves
Alexander, Kimberly A.; Warnick, Lorin D.; Cripps, Chris J.; McDonough, Patrick L.; Grohn, Yrjo T.; Wiedmann, Martin; Reed, Kelly E.; James, Karen L.; Soyer, Yeşim; Ivanek, Renata (American Veterinary Medical Association (AVMA), 2009-09-15)
Objective-To determine the duration of fecal shedding of and serologic response to Salmonella spp after natural infection in dairy calves and characterize Salmonella organisms recovered from these herds.
Development of immunoaffinity based detection platforms for food pathogens
Çam, Dilek; Öktem, Hüseyin Avni; Department of Biology (2016)
Salmonella and E.coli, food pathogens, are among the very important pathogens threating the health. Rapid and easy detection of these pathogens is crucial. In this context, lateral flow assay (LFA) platform prepared by gold nanoparticles (GNPs) based on antibody for Salmonella and E.coli O157:H7 was developed in the first part of this study. In second part, single chain variable fragments (scFv) and SNAP-Tagged full IgG (fusion protein) of se155-4 antibody specific to Salmonella were genetically produced. T...
Effect of FMD vaccination schedule of dams on the level and duration of maternally derived antibodies
Sareyyupoglu, B.; Gulyaz, V.; Cokcaliskan, C.; Unal, Y.; Cokulgen, T.; Uzunlu, E.; Gurcan, S.; İlk Dağ, Özlem (Elsevier BV, 2019-11-01)
Vaccination against Foot and Mouth Disease (FMD) in pregnant cows is crucial to produce greater immunity in new born calves, especially in late gestation, as this directly affects neonatal immunity. Therefore, we aimed to investigate how late gestation FMD vaccination of pregnant cows affects the maternally derived antibodies in their offspring. Pregnant cows were vaccinated with and without booster vaccination during the 3rd months (early gestation vaccination, EGV) or the 6.5th months (late gestation vacc...
Subtyping of salmonella isolated from human clinical and animal non-clinical cases, as well as different food samples using pulsed field gel electrophoresis (PFGE) /
Bulut, Ece; Soyer, Yeşim; Özcengiz, Gülay; Department of Biotechnology (2014)
Salmonella is one of the most reported pathogens in foodborne outbreaks worldwide. In order to ensure safety of foods, farm-to-fork surveillance and control systems must be utilized. Development of numerous typing methods have improved the ability to detect salmonellosis outbreaks, enabling to trace the contaminated source from farm to fork. Facilitating prevention and regulation of techniques, knowledge of the epidemiology, genetics and ecology of Salmonella infections depend on the information provided by...
Microbial growth and attachment of Salmonella and enterohemorrhagic and enteroaggregative Escherichia coli strains on cress microgreens grown in peat soil system
Namlı, Şahin; Samut, Hilal; Soyer, Yeşim (2021-12-01)
Purpose This study aimed to investigate how enteric pathogens and their biofilm populations on fresh produce survive according to time that contamination has occurred on leaves and contamination route: seed irrigation water. Design/methodology/approach Cress was contaminated in two different ways: contamination of seeds and irrigation water with 8-log MPN/mL bacterial load, Salmonella Newport, Escherichia coli O157:H7, O104:H4 or O78:H2. While contaminated seeds were cultivated for seed contamination, conta...
Citation Formats
A. Cesur, S. O. Ulutas, and Y. Soyer, “Isolation and molecular characterization of Salmonella enterica and Escherichia coli from poultry samples,” TURKISH JOURNAL OF VETERINARY & ANIMAL SCIENCES, pp. 408–422, 2019, Accessed: 00, 2020. [Online]. Available: