A novel conducting polymer based platform for ethanol sensing

Date

2014-03-01

Author

Kekec, Nur Cicek
Kanik, Fulya Ekiz
UDUM, YASEMİN
HIZLIATEŞ, CEVHER GÜNDOĞDU
ERGÜN, Mustafa Yavuz
Toppare, Levent Kamil

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Anew amperometric ethanol biosensor was fabricated for sensitive and rapid analysis of ethanol. For this purpose, a novel monomer, 9-methyl-9H-carbazole-3-carbohydrazine (MCCH), was designed and electrochemically polymerized on a graphite electrode to achieve a conducting and effective immobilization matrix for enzyme immobilization. Due to the presence of amino groups in the structure of conducting polymer, alcohol oxidase molecules were covalently immobilized onto the functional polymer which brought an effective and long-life analysis of the substrate, ethanol. N-(3-Dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride (EDC)/N-hydroxysuccinimide (NHS) chemistry was used for linking carboxylic acid groups of the enzyme molecules with amino groups of the polymer. After successful immobilization, amperometric biosensor responses were recorded at 0.7V vs. Ag/AgCl in phosphate buffer (pH 7.0). K-M(app) (8.74 mM), I-max (5.94 mu A), LOD (0.131 mM) and sensitivity (4.79 mu A mM(-1) cm(-2)) values were determined. Finally, the prepared biosensor was successfully applied for determination of ethanol content in alcoholic beverages.

Subject Keywords

Conducting polymer, Ethanol biosensor, Alcohol oxidase, Covalent immobilization

URI

https://hdl.handle.net/11511/48812

Journal

SENSORS AND ACTUATORS B-CHEMICAL

DOI

https://doi.org/10.1016/j.snb.2013.12.007

Collections

Department of Chemistry, Article

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Citation Formats

N. C. Kekec, F. E. Kanik, Y. UDUM, C. G. HIZLIATEŞ, M. Y. ERGÜN, and L. K. Toppare, “A novel conducting polymer based platform for ethanol sensing,” SENSORS AND ACTUATORS B-CHEMICAL, pp. 306–314, 2014, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/48812.