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A novel conducting polymer based platform for ethanol sensing
Date
2014-03-01
Author
Kekec, Nur Cicek
Kanik, Fulya Ekiz
UDUM, YASEMİN
HIZLIATEŞ, CEVHER GÜNDOĞDU
ERGÜN, Mustafa Yavuz
Toppare, Levent Kamil
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Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License
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Anew amperometric ethanol biosensor was fabricated for sensitive and rapid analysis of ethanol. For this purpose, a novel monomer, 9-methyl-9H-carbazole-3-carbohydrazine (MCCH), was designed and electrochemically polymerized on a graphite electrode to achieve a conducting and effective immobilization matrix for enzyme immobilization. Due to the presence of amino groups in the structure of conducting polymer, alcohol oxidase molecules were covalently immobilized onto the functional polymer which brought an effective and long-life analysis of the substrate, ethanol. N-(3-Dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride (EDC)/N-hydroxysuccinimide (NHS) chemistry was used for linking carboxylic acid groups of the enzyme molecules with amino groups of the polymer. After successful immobilization, amperometric biosensor responses were recorded at 0.7V vs. Ag/AgCl in phosphate buffer (pH 7.0). K-M(app) (8.74 mM), I-max (5.94 mu A), LOD (0.131 mM) and sensitivity (4.79 mu A mM(-1) cm(-2)) values were determined. Finally, the prepared biosensor was successfully applied for determination of ethanol content in alcoholic beverages.
Subject Keywords
Conducting polymer
,
Ethanol biosensor
,
Alcohol oxidase
,
Covalent immobilization
URI
https://hdl.handle.net/11511/48812
Journal
SENSORS AND ACTUATORS B-CHEMICAL
DOI
https://doi.org/10.1016/j.snb.2013.12.007
Collections
Department of Chemistry, Article
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N. C. Kekec, F. E. Kanik, Y. UDUM, C. G. HIZLIATEŞ, M. Y. ERGÜN, and L. K. Toppare, “A novel conducting polymer based platform for ethanol sensing,”
SENSORS AND ACTUATORS B-CHEMICAL
, pp. 306–314, 2014, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/48812.