Development of a new ethanol biosensor based on polyfluorene- g- poly(ethyleneglycol) and multiwalled carbon nanotubes

Download

12626274.pdf

Date

2021-2-5

Author

Deniz, Sıddıka Aybüke

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

313
views

302
downloads

In this thesis study, a novel amperometric biosensor containing a conjugated polymer and a multiwalled carbon nanotube was developed for ethanol detection. Conjugated polymers have gained interest in biosensing and biomedical applications recently. Their ability to increase the effective surface area and adjustable surface morphology make them convenient candidates for suitable enzyme immobilization. The conjugated polymer poly(ethylene glycol) is soluble in both water and organic solvents. Besides, it interacts with the enzymes readily due to its hydrophilic nature. In this thesis, poly(ethylene glycol) with fluorene functionality was synthesized via a one-step procedure. The construction of biosensor was achieved through the modification of graphite electrode with synthesized conjugated polymer in addition to carbon nanotubes. Carbon nanotubes were used as supporting materials due to their fast electron transfer and electrochemical stability. Surface characterization and electrochemical characterization of the biosensor was performed using scanning electron microscopy and cyclic voltammetry, respectively. Combination of conjugated polymer and multiwalled carbon nanotubes improved the performance of biosensor and showed high sensing ability compare to similar control biosensor (without PF-g-PEG or MWCNT). Alcohol oxidase enzyme (AOx) was immobilized on the modified electrode surface and several parameters effecting the performance of modified biosensor were optimized. The optimized biosensor exhibited good analytical parameterssuch as low limit of detection and high sensitivity. Linear range of proposed biosensor was between 0.0085-5.95 mM with detection limit of 0.11 mM and sensitivity value of 7.99 µAmM-1 cm-2 . Finally, to show the applicability of proposed biosensor, the biosensor was tested for different alcoholic beverages for ethanol analysis.

Subject Keywords

Amperometric ethanol biosensor, Conjugated polymers, Alcohol oxidase, Carbon nanotubes, Polyfluorene

URI

https://hdl.handle.net/11511/89715

Collections

Graduate School of Natural and Applied Sciences, Thesis

Suggestions

OpenMETU
Core

Fabrication of a promising immobilization platform based on electrochemical synthesis of a conjugated polymer Buber, Ece; SÖYLEMEZ, SANİYE; UDUM, YASEMİN; Toppare, Levent Kamil (2018-07-01) Since conjugated polymers are an important class of materials with remarkable properties in biosensor applications, in this study, a novel glucose biosensor based on a conjugated polymer was fabricated via the electropolymerization of the monomer 10,13-bis(4-hexylthiophen-2-yl)dipyridol[3,2-a:2',3'-c]phenazine onto a graphite electrode surface. Glucose oxidase (GOx) was used as the model biological recognition element. As a result of the enzymatic reaction between GOx and glucose, the glucose amount was det...
A novel conducting polymer based platform for ethanol sensing Kekec, Nur Cicek; Kanik, Fulya Ekiz; UDUM, YASEMİN; HIZLIATEŞ, CEVHER GÜNDOĞDU; ERGÜN, Mustafa Yavuz; Toppare, Levent Kamil (2014-03-01) Anew amperometric ethanol biosensor was fabricated for sensitive and rapid analysis of ethanol. For this purpose, a novel monomer, 9-methyl-9H-carbazole-3-carbohydrazine (MCCH), was designed and electrochemically polymerized on a graphite electrode to achieve a conducting and effective immobilization matrix for enzyme immobilization. Due to the presence of amino groups in the structure of conducting polymer, alcohol oxidase molecules were covalently immobilized onto the functional polymer which brought an e...
A conducting polymer and a calixarene derivative A novel surface design for glucose detection Gökoğlan, Ceren; Söylemez, Saniye; Kesik, Melis; Ünay, Hande; Sayın, Serkan; Çırpan, Ali; Yıldız, Hüseyin Bekir; Toppare, Levent Kamil (null; 2016-07-17) In this study, a novel amperometric glucose biosensor based on a conducting polymer and a calixarene was developed. Conducting polymer of (2‐(2‐oc‐tyldodecyl)‐4,7‐di(selenoph‐2‐yl)‐2H‐benzo[d][1,2,3]triazole)) (SBTz) was used as the immobilization matrix for biomolecule deposition to achieve an effective surface design to detect glucose. After successful deposition of SBTz on graphite electrode, a newly synthesized calixarene and gold nanoparticle (AuNP) mixture were used for improving biosensor character...
Development of an amperometric biosensor based on a novel conducting copolymer for detection of anti-dementia drugs Turan, Janset; Kesik, Melis; Soylemez, Saniye; GÖKER, SEZA; Kolb, Marit; Bahadir, Mufit; Toppare, Levent Kamil (2014-12-01) In this study, a new amperometric biosensor was developed for the detection of the anti-dementia drugs fortified with tap water. For this purpose, electrocopolymerization of 5,6-bis(octyloxy)-4,7-di(thiophen2-yl)benzo[c][1,2,5]oxadiazole(BODT) with (2-(((9H-fluoren-9-yl)methoxy)carbonylamino) acetic acid (FMOC) on graphite electrode was successfully achieved and used as an immobilization matrix. Acetylcholinesterase (AChE) and choline oxidase (ChO) enzyme couple was immobilized on copolymer coated graphite ...
A new ethanol biosensor based on polyfluorene-g-poly(ethylene glycol) and multiwalled carbon nanotubes Bekmezci, Siddika Aybuke; SÖYLEMEZ, SANİYE; Yilmaz, Gorkem; Udum, Yasemin A.; Yağcı, Yusuf; Toppare, Levent Kamil (Elsevier BV, 2020-01-05) Functionalization of the conjugated polymers has gained considerable interest in the biomedical engineering and biosensing applications as distinct properties can be imparted to the corresponding pristine analogues. In the present work, we report a novel sensing platform for the quantification of ethanol through a macromolecular design involving polyfluorene-g-poly(ethylene glycol) (PF-g-PEG) and multiwalled carbon nanotubes (MWCNTs). First, poly(ethylene glycol) with fluorene functionality (PEG-FL) was syn...

Citation Formats

S. A. Deniz, “Development of a new ethanol biosensor based on polyfluorene- g- poly(ethyleneglycol) and multiwalled carbon nanotubes,” M.S. - Master of Science, Middle East Technical University, 2021.