Development of an amperometric biosensor based on a novel conducting copolymer for detection of anti-dementia drugs

Turan, Janset
Kesik, Melis
Soylemez, Saniye
Kolb, Marit
Bahadir, Mufit
Toppare, Levent Kamil
In this study, a new amperometric biosensor was developed for the detection of the anti-dementia drugs fortified with tap water. For this purpose, electrocopolymerization of 5,6-bis(octyloxy)-4,7-di(thiophen2-yl)benzo[c][1,2,5]oxadiazole(BODT) with (2-(((9H-fluoren-9-yl)methoxy)carbonylamino) acetic acid (FMOC) on graphite electrode was successfully achieved and used as an immobilization matrix. Acetylcholinesterase (AChE) and choline oxidase (ChO) enzyme couple was immobilized on copolymer coated graphite electrode via covalent binding with the help of carbodiimide chemistry. Changes in the responses of the proposed biosensor based on AChE inhibition were recorded using acetylcholine as the substrate. The bi-enzymatic biosensor based on conducting copolymer showed good linear detection range between 0.01 and 12.0 mM and a detection limit (LOD) of 0.014 mM to acetylcholine. Surface and electrochemical characterization were performed via Scanning Electron Microscopy (SEM) and cyclic voltammetry (CV) techniques. Moreover, the design biosensor system was tested for the detection of neostigmine and donepezil as pharmaceuticals in fortified tap water samples. Very low detection limits of 0.027 mu g/L donepezil and 0.559 mu g/L neostigmine were achieved. The analysis of spiked tap water proved the biosensor capability to be used. The results were found to be in good agreement with the ones determined by HPLC/DAD technique.


Development of a new ethanol biosensor based on polyfluorene- g- poly(ethyleneglycol) and multiwalled carbon nanotubes
Deniz, Sıddıka Aybüke; Toppare, Levent Kamil; Söylemez, Saniye; Department of Chemistry (2021-2-5)
In this thesis study, a novel amperometric biosensor containing a conjugated polymer and a multiwalled carbon nanotube was developed for ethanol detection. Conjugated polymers have gained interest in biosensing and biomedical applications recently. Their ability to increase the effective surface area and adjustable surface morphology make them convenient candidates for suitable enzyme immobilization. The conjugated polymer poly(ethylene glycol) is soluble in both water and organic solvents. Besides, it inte...
Employing Imino-Bis-Propane Diol Functional Polymers for Boron Removal from Geothermal Waters via Polymer Enhanced Ultrafiltration
Zerze, Hasan; Ozbelge, H. Onder; Bicak, Niyazi; Yılmaz, Levent (2015-09-02)
In this study, polymer enhanced ultrafiltration (PEUF) was used to remove boron from a geothermal water sample. The effect of co-ions such as chloride and sulfate on boron removal was investigated using previously synthesized poly (vinyl amino-N, N'-bis-propane diol) (GPVA) and its copolymers with DADMAC. The presence of co-anions and use of real waste water did not significantly reduce boron retentions and permeate fluxes. At optimum operating conditions 98% boron removal was achieved. It was demonstrated ...
Aerobic biological treatment of opium alkaloid wastewater-effect of gamma radiation and fenton's oxidation as pretreatment
Bural, Cavit Burak; Dilek, Filiz Bengü; Department of Environmental Engineering (2008)
In this study, aerobic biological treatment of opium alkaloid wastewater and the effect of gamma preirradiation and fenton’s oxidation were investigated. First, the biodegradability of alkaloid wastewater was investigated by batch reactors and wastewater was found to be highly biodegradable providing 83 90 % COD degradation. In order to evaluate the effect of irradiation, original wastewater and irradiated wastewaters (40 & 140 kGy) were compared by means of BOD5/COD values and through aerobic batch experi...
Development of a synthetic strategy for Water soluble tripodal receptors: Two novel fluorescent receptors for highly selective and sensitive detections of Fe3+ and Cu2+ ions and biological evaluation
USLU, AYLİN; Ozcan, Elif; TÜMAY, SÜREYYA OĞUZ; Kazan, Hasan Huseyin; YEŞİLOT, SERKAN (Elsevier BV, 2020-04-01)
A general synthetic strategy is developed to synthesize water soluble receptors by employing tripodal system based on a cyclotriphosphaze platform. The developed model is successfully synthesized and characterized by using elemental analysis, FT-IR, MALDI-TOF, H-1 NMR, C-13 NMR and P-31 NMR techniques. The fluorescence sensing performance of prepared water soluble tripodal systems were evaluated by UV/Vis and fluorescence spectroscopies. According to obtained results, two novel water-soluble sensing platfor...
An effective surface design based on a conjugated polymer and silver nanowires for the detection of paraoxon in tap water and milk
Turan, Janset; Kesik, Melis; SÖYLEMEZ, SANİYE; Goker, Seza; Coskun, Sahin; Ünalan, Hüsnü Emrah; Toppare, Levent Kamil (2016-06-02)
In this study, a novel approach for the fabrication of a biosensor utilizing a conducting polymer and silver nanowires is reported. To obtain immobilization platform for butyrylcholinesterase (BChE), a graphite electrode was modified with the poly(5,6-bis(octyloxy)-4,7-di(thieno[3][3,2-b]thiophen-2yl)benzo[c][1,2,5]oxoadiazole) (PTTBO) which has a hydrophobic alkyl chain as the pendant group providing hydrophobic nature to the matrix. Since biomolecules contain both hydrophobic and hydrophilic parts in thei...
Citation Formats
J. Turan et al., “Development of an amperometric biosensor based on a novel conducting copolymer for detection of anti-dementia drugs,” JOURNAL OF ELECTROANALYTICAL CHEMISTRY, pp. 43–50, 2014, Accessed: 00, 2020. [Online]. Available: