2-Amino-2-deoxy-glucose conjugated cobalt ferrite magnetic nanoparticle (2DG-MNP) as a targeting agent for breast cancer cells

Date

2016-01-01

Author

Asik, Elif
Aslan, Tugba Nur
Volkan, Mürvet
Güray, Nülüfer Tülün

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

70
views

0
downloads

In this study, 2-amino-2-deoxy-glucose (2DG) was conjugated to COOH modified cobalt ferrite magnetic nanoparticles (COOH-MNPs), which were designed to target tumor cells as a potential targetable drug/gene delivery agent for cancer treatment. According to our results, it is apparent that, 2DG labeled MNPs were internalized more efficiently than COOH-MNPs under the same conditions in all cell types (MDA-MB-231 and MCF-7 cancer and MCF-10A normal breast cells) (p < 0.001). Moreover, the highest amount of uptake was observed in MDA-MB-231, followed by MCF-7 and normal MCF-10A cells for both MNPs. The apoptotic effects of 2DG-MNPs were further evaluated, and it was found that apoptosis was not induced at low concentrations of 2DG-MNPs in all cell types, whereas dramatic cell death was observed at higher concentrations. In addition, the gene expression levels of four drug-metabolizing enzymes, two Phase I (CYP1A1, CYP1B1) and two Phase II (GSTM3, GSTZ1) were also increased with the high concentrations of 2DG-MNPs.

Subject Keywords

2-Amino-2-deoxy-glucose, Cobalt ferrite nanoparticles, Breast cancer, Targeting therapy

URI

https://hdl.handle.net/11511/49106

Journal

ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY

DOI

https://doi.org/10.1016/j.etap.2015.12.004

Collections

Department of Chemistry, Article

Suggestions

OpenMETU
Core

Superior Photodynamic Therapy of Colon Cancer Cells by Selenophene-BODIPY-Loaded Superparamagnetic Iron Oxide Nanoparticles Ozvural Sertcelik, Kubra Nur; Karaman, Osman; Almammadov, Toghrul; Günbaş, Emrullah Görkem; Kolemen, Safacan; Yagci Acar, Havva; Onbasli, Kubra (2022-01-01) © 2022 Wiley-VCH GmbH.Development of targeted nanoparticles as carriers to deliver photosensitizers to cancer cells is highly beneficial for ensuring the expected therapeutic outcome of photodynamic therapy. Herein, polyacrylic acid (PAA) coated superparamagnetic iron oxide nanoparticles (SPIONs), conjugated with endothelial growth factor receptor (EGFR) targeting Cetuximab (Cet) were loaded with a BODIPY-based (BOD-Se-I) photosensitizer (Cet-PAA@SPION/BOD-Se-I) to achieve enhanced and selective photodynami...
Targeted delivery of CPG-oligodeoxynucleotide to breast cancer cells by poly-amidoamine dendrimer-modified magnetic nanoparticles Taghavi Pourianazar, Negar; Gündüz, Ufuk; Gündüz, Güngör; Department of Biotechnology (2016) One major application of nanotechnology in cancer treatment involves designing nanoparticles to deliver drugs, oligonucleotides, and genes to cancer cells. Nanoparticles should be engineered so that they could target and destroy tumor cells with minimal damage to healthy tissues. This research aims to develop an appropriate and efficient nanocarrier, having the ability of interacting with and delivering CpG-oligodeoxynucleotides (CpG-ODNs) to tumor cells. CpG-ODNs activate Toll-like receptor 9 (TLR9), which...
Kanser hedefleme ve terapi için 2-deoksi-D-gilikoz (2-DG)-etiketli kobalt ferrit (CoFe2O4)manyetik nanoparçacıklar Işık, Elif; Güray, Nülüfer Tülün; Volkan, Mürvet(2014) In this study, strong magnetic featured silica coated Cobalt ferrite (CoFe204) nanoparticles were labeled with 2-deoxy glucose (2-DG) for better targetting to cancer cells and the molecular effects of these prepared magnetic nanoparticles (MNPs) were investigated in cancer cell lines. In this study, in vitro characterization of 2-DG labelled MNPs were carried out in breast cancer cells (MDA-MB-231 breast cancer and normal cell MCF10A), and cellular uptake mechanisms, genotoxicity, intracellular levels of ox...
Development of poly (I:C) modified doxorubicin loaded magnetic dendrimer nanoparticles for targeted combination therapy Khodadust, Rouhollah; Unsoy, Gozde; Gündüz, Ufuk (2014-10-01) The objective of this study was to develop and evaluate the anticancer activity and the safety of a combinational drug delivery system using polyamidoamine (PAMAM) dendrimer-coated iron oxide nanoparticles for doxorubicin and poly I:C delivery in vitro. Dendrimer-coated magnetic nanoparticles (DcMNPs) are suitable for drug delivery system as nanocarriers with their following properties, such as surface functional groups, symmetry perfection, internal cavities, nano-size and magnetization. These nanoparticle...
Synthesis and characterization of polymeric magnetic nanoparticles loaded by gemcitabine / Parsian, Maryam; Gündüz, Ufuk; Tezcaner, Ayşen; Department of Biotechnology (2014) In this study, different types of magnetic nanoparticles were synthesized for treatment of breast cancer by targeted drug delivery. Polyamidoamine (PAMAM) dendrimer, Chitosan (CS) and Polyhydroxybutyrate (PHB) coated magnetic nanoparticles were prepared and loaded with Gemcitabine. The loading efficiency of drug for various half generations of dendrimer coated magnetic nanoparticles (DcMNPs), Chitosan coated magnetic nanoparticles (CSMNPs) and Polyhydroxybutyrate magnetic nanoparticles (PHB-MNPs) were inves...

Citation Formats

E. Asik, T. N. Aslan, M. Volkan, and N. T. Güray, “2-Amino-2-deoxy-glucose conjugated cobalt ferrite magnetic nanoparticle (2DG-MNP) as a targeting agent for breast cancer cells,” ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY, pp. 272–278, 2016, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/49106.