2-Amino-2-deoxy-glucose conjugated cobalt ferrite magnetic nanoparticle (2DG-MNP) as a targeting agent for breast cancer cells

Date

2016-01-01

Author

Asik, Elif
Aslan, Tugba Nur
Volkan, Mürvet
Güray, Nülüfer Tülün

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This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

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In this study, 2-amino-2-deoxy-glucose (2DG) was conjugated to COOH modified cobalt ferrite magnetic nanoparticles (COOH-MNPs), which were designed to target tumor cells as a potential targetable drug/gene delivery agent for cancer treatment. According to our results, it is apparent that, 2DG labeled MNPs were internalized more efficiently than COOH-MNPs under the same conditions in all cell types (MDA-MB-231 and MCF-7 cancer and MCF-10A normal breast cells) (p < 0.001). Moreover, the highest amount of uptake was observed in MDA-MB-231, followed by MCF-7 and normal MCF-10A cells for both MNPs. The apoptotic effects of 2DG-MNPs were further evaluated, and it was found that apoptosis was not induced at low concentrations of 2DG-MNPs in all cell types, whereas dramatic cell death was observed at higher concentrations. In addition, the gene expression levels of four drug-metabolizing enzymes, two Phase I (CYP1A1, CYP1B1) and two Phase II (GSTM3, GSTZ1) were also increased with the high concentrations of 2DG-MNPs.

Subject Keywords

2-Amino-2-deoxy-glucose, Cobalt ferrite nanoparticles, Breast cancer, Targeting therapy

URI

https://hdl.handle.net/11511/49106

Journal

ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY

DOI

https://doi.org/10.1016/j.etap.2015.12.004

Collections

Department of Chemistry, Article

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Citation Formats

E. Asik, T. N. Aslan, M. Volkan, and N. T. Güray, “2-Amino-2-deoxy-glucose conjugated cobalt ferrite magnetic nanoparticle (2DG-MNP) as a targeting agent for breast cancer cells,” ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY, pp. 272–278, 2016, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/49106.