Low dose simvastatin induces compositional, structural and dynamic changes in rat skeletal extensor digitorum longus muscle tissue

Date

2009-10-6

Author

Ozek Simsek, Nihal
Sara, Yildirim
Onur, Rustu
Severcan, Feride

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Statins are commonly used drugs in the treatment of hypercholesterolaemia. There are many adverse effects of statins on skeletal muscle, but the underlying mechanisms remain unclear. In the present study, the effects of low dose (20 mg/kg) simvastatin, a lipophilic statin, on rat EDL muscle (extensor digitorum longus muscle) were investigated at the molecular level using FTIR (Fourier-transform infrared) spectroscopy. FTIR spectroscopy allows us rapid and sensitive determination of functional groups belonging to proteins, lipids, carbohydrates and nucleic acids simultaneously. The results revealed that simvastatin treatment induces a significant decrease in lipid, nucleic acid, protein and glycogen content. A significant increase in the lipid/protein and nucleic acid/protein ratios was also obtained with simvastatin treatment. Furthermore, an increase in lipid order and membrane fluidity was detected. A decrease in the bandwidth of the amide I band and shifting of the position of this band to higher frequency values in treated muscle indicates structural changes in proteins. Detailed secondary structure analysis of the amide I band revealed a significant increase in antiparallel and aggregated β-sheet, random coil structure and a significant decrease in β-sheet structure, which indicates protein denaturation.

Subject Keywords

Biophysics, Cell Biology, Biochemistry, Molecular Biology

URI

https://hdl.handle.net/11511/50944

Journal

Bioscience Reports

DOI

https://doi.org/10.1042/bsr20080150

Collections

Department of Biology, Article

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Citation Formats

N. Ozek Simsek, Y. Sara, R. Onur, and F. Severcan, “Low dose simvastatin induces compositional, structural and dynamic changes in rat skeletal extensor digitorum longus muscle tissue,” Bioscience Reports, pp. 41–50, 2009, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/50944.