FTIR Spectroscopic Investigation of Mineral Structure of Streptozotocin Induced Diabetic Rat Femur and Tibia

Boyar, Handan
Turan, Belma
Severcan, Feride
Diabetes mellitus (DM) can be accepted as a heterogenous multi organ disorder that can affect various systems of the human body. Disorders include retinopathy, neuropathy, cardiomyopathy, musculoskeletal abnormalities such as diminished bone formation and bone healing retardation. Low bone mineral density is often mentioned as a complication for patients with insulin dependent diabetes mellitus (type I DM). Streptozotocin (STZ) induced diabetic rats are good models for investigation of the complications of insulin dependent diabetes. In the present study, the effects of STZ induced diabetes on the mineral environment of rat bones namely femur and tibia were studied by Fourier transform infrared (FTIR) spectroscopic technique. The results revealed that mineral crystal sizes increased and carbonate content decreased for diabetic femur and tibia. These changes can be due to the formation of osteoporosis which is widely seen in diabetic patients.


FTIR imaging of structural changes in visceral and subcutaneous adiposity and brown to white adipocyte transdifferentiation
Baloglu, Fatma Kucuk; Garip, Sebnem; Heise, Sebastian; Brockmann, Gudrun; Severcan, Feride (Royal Society of Chemistry (RSC), 2015-01-01)
Obesity is a heterogeneous disorder which increases risks for multiple metabolic diseases, such as type 2 diabetes. The current study aims to characterize and compare visceral and subcutaneous adipose tissues in terms of macromolecular content and investigate transdifferentiation between white and brown adipocytes. Regarding this aim, Fourier transform infrared (FTIR) microspectroscopy and uncoupling protein 1 (UCP1) immunohistological staining were used to investigate gonadal (visceral) and inguinal (subcu...
Electrophysiologic vestibular evaluation in type 2 diabetic and prediabetic patients: Air conduction ocular and cervical vestibular evoked myogenic potentials
Konukseven, Ozlem; Polat, Sefi Ka Burcak; KARAHAN, SEVİLAY; Konukseven, Erhan İlhan; Ersoy, Reyhan; Cakir, Bekir; Kutluhan, Ahmet; AKSOY, SONGÜL (2015-01-01)
Objective: Chronically increased blood glucose levels may affect the vestibular system by damaging cells and neural structures in diabetes mellitus (DM). We aimed to search the effects of neurovascular degeneration on the vestibular system in type 2 DM and prediabetic patients by using air-conducted ocular (oVEMP) and cervical (cVEMP) vestibular evoked myogenic potentials. Design: Prospective study. Study sample: Thirty diabetic, 30 prediabetic patients, and 31 age-and sex-matched controls having no periphe...
Fourier transform infrared study of the effect of diabetes on rat liver and heart tissues in the C-H region
Severcan, Feride; Toyran, N; Kaptan, N; Turan, B (2000-10-02)
Diabetes mellitus is characterized by hyperglycemia, a relative lack of insulin. The metabolic disturbances in diabetic patients are often associated with cardiac and liver dysfunctions. Generally, experimental diabetic models in animals have been used to study diabetes-related changes in organ function, but the complexity of intact tissues can cause contradictory results. For this reason, different techniques have been used to understand the mechanisms of these dysfunctions in diabetic organs. The purpose ...
Investigation of diabetes-induced effect on apex of rat heart myocardium by using cluster analysis and neural network approach: An FTIR study
Toyran, Neslihan; Severcan, Feride; Severcan, Mete; Turan, Belma (Hindawi Limited, 2007)
Diabetes mellitus (DM) is a progressive chronic disorder, which affects people belonging to all age groups of the population. This disease is accompanied by a greatly increased risk of cardiovascular death. In the present study, the effects of streptozotocin (STZ)-induced type 1 diabetes on apex myocardium of the rat heart have been investigated using Fourier Transform Infrared (FTIR) spectra. The cluster analysis has been applied to FTIR spectra to differentiate the diabetic samples from the normal control...
FTIR Microspectroscopy: A Multiple-screening Platform for Investigating Single-cell Biochemical Perturbations upon Prion Infection
Didonna, Alessandro; Vaccari, Lisa; Bek, Alpan; Legname, Giuseppe (2010-07-01)
Prion diseases are a group of fatal neurodegenerative disorders characterized by the accumulation of prions in the central nervous system. The pathogenic prion (PrPSc) possesses the capability to replicate into nascent PrPSc from the host-encoded cellular isoform of the prion protein. The present work aims at providing novel insight into cellular response upon prion infection, as evidenced by Synchrotron Radiation InfraRed MicroSpectroscopy (SR-IRMS). This non-invasive, label-free analytical technique was e...
Citation Formats
H. Boyar, B. Turan, and F. Severcan, “FTIR Spectroscopic Investigation of Mineral Structure of Streptozotocin Induced Diabetic Rat Femur and Tibia,” Spectroscopy, pp. 627–633, 2003, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/51746.